cDNA Clone ALPK1

• Plasmid: pcDNA5-FRT-TO-MBP-3FLAG-ALPK1 K1067M
• Parent Plasmid: pcDNA5-FRT-TO-MBP
• Expressed: MBP-3FLAG-ALPK1 K1067M
• Genbank: 80216
• Tag: FLAG
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCcATGAATAATCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAAGCAAGTGCTGGATCAGCTCTTGTTGGAAGCGCCAGATGTGTCGGAAGAGGACAAGAGCGAGGACCAGCGCTGCAGAGCTTTACTCCCCAGCGAGTTAAGGACCCTGATCCAGGAGGCAAAGGAAATGAAGTGGCCCTTCGTGCCTGAAAAGTGGCAGTACAAACAAGCCGTGGGCCCAGAGGACAAAACAAACCTGAAGGATGTGATTGGCGCCGGGTTGCAGCAGTTACTGGCGTCCCTGAGGGCCTCCATCCTCGCTCGGGACTGTGCGGCTGCGGCGGCTATTGTGTTCTTGGTGGACCGGTTCCTGTATGGGCTCGACGTCTCTGGAAAACTTCTGCAGGTCGCCAAAGGTCTCCACAAGTTGCAGCCAGCCACGCCAATTGCCCCGCAGGTGGTTATTCGCCAAGCCCGAATCTCCGTGAACTCAGGAAAACTTTTAAAAGCAGAGTATATTCTGAGCAGTCTAATAAGCAACAATGGAGCAACGGGTACCTGGCTGTACAGAAATGAAAGTGACAAGGTCCTGGTGCAGTCGGTCTGTATACAGATCAGAGGGCAGATTCTGCAAAAGCTGGGGATGTGGTACGAAGCAGCAGAGTTAATATGGGCCTCCATTGTAGGATATTTGGCACTTCCTCAGCCGGATAAAAAGGGCCTCTCCACGTCGCTAGGTATACTGGCAGACATCTTTGTTTCCATGAGCAAGAACGATTATGAAAAGTTTAAAAACAATCCACAAATTAATTTGAGCCTGCTGAAGGAGTTTGACCACCATTTGCTGTCCGCTGCAGAAGCCTGCAAGCTGGCAGCTGCCTTCAGTGCCTATACGCCGCTCTTCGTGCTCACAGCTGTGAATATCCGTGGCACGTGTTTATTGTCCTACAGTAGTTCAAATGACTGTCCTCCAGAATTGAAAAACTTACATCTGTGTGAAGCCAAAGAGGCCTTTGAGATTGGCCTCCTCACCAAGAGAGATGATGAGCCTGTTACTGGAAAACAGGAGCTTCACAGCTTTGTCAAAGCTGCTTTCGGTCTCACCACAGTGCACAGAAGGCTCCATGGGGAGACAGGGACGGTCCATGCAGCAAGTCAGCTCTGTAAGGAAGCAATGGGGAAGCTGTACAATTTCAGCACTTCCTCCAGAAGTCAGGACAGAGAAGCTCTGTCTCAAGAAGTTATGTCTGTGATTGCCCAGGTGAAGGAACATTTACAAGTTCAAAGCTTCTCAAATGTAGATGACAGATCTTATGTTCCCGAGAGTTTCGAGTGCAGGTTGGATAAACTTATCTTGCATGGGCAAGGGGATTTCCAAAAAATCCTTGACACCTATTCACAGCACCATACTTCGGTGTGTGAAGTATTTGAAAGTGATTGTGGAAACAACAAAAATGAACAGAAAGATGCAAAAACAGGAGTCTGCATCACTGCTCTAAAAACAGAAATAAAAAACATAGATACTGTGAGTACTACTCAAGAAAAGCCACATTGTCAAAGAGACACAGGAATATCTTCCTCCCTAATGGGTAAGAATGTTCAGAGGGAACTCAGAAGGGGAGGAAGGAGAAACTGGACCCATTCTGATGCATTTCGAGTCTCCTTGGATCAAGATGTGGAGACTGAGACTGAGCCATCGGACTACAGCAATGGTGAGGGAGCTGTTTTCAACAAGTCTCTGAGTGGCAGCCAGACTTCCAGTGCTTGGAGCAACTTATCAGGGTTTAGTTCCTCTGCAAGCTGGGAGGAAGTGAATTATCACGTTGACGACAGGTCAGCCAGAAAAGAGCCTGGCAAAGAACATCTGGTGGACACTCAGTGTTCCACTGCCTTGTCTGAGGAGCTAGAGAATGACAGGGAAGGCAGAGCTATGCATTCATTGCATTCACAGCTTCATGATCTCTCTCTTCAGGAACCCAACAATGACAATTTGGAGCCTTCTCAAAATCAGCCACAGCAACAGATGCCCTTGACACCCTTCTCGCCTCATAATACCCCAGGCATTTTCTTGGCCCCTGGTGCAGGGCTTCTAGAAGGAGCTCCAGAAGGTATCCAGGAAGTCAGAAATATGGGACCCAGAAATACTTCTGCTCACTCCAGACCCTCATATCGTTCTGCTTCTTGGTCTTCTGATTCTGGTAGGCCCAAGAATATGGGCACACATCCTTCAGTCCAAAAAGAAGAAGCCTTTGAAATAATTGTTGAGTTTCCAGAAACCAACTGCGATGTCAAAGACAGGCAGGGGAAAGAGCAGGGAGAAGAAATTAGTGAAAGAGGCGCAGGCCCTACATTTAAAGCTAGTCCCTCCTGGGTTGACCCAGAAGGAGAAACAGCAGAAAGCACTGAAGATGCACCCTTAGACTTTCACAGGGTCCTGCACAATTCTCTGGGAAACATTTCCATGCTGCCATGTAGCTCCTTCACCCCTAATTGGCCTGTTCAAAATCCTGACTCCAGAAAAAGTGGTGGCCCAGTCGCAGAGCAGGGCATCGACCCTGATGCCTCCACAGTGGATGAGGAGGGGCAACTGCTCGACAGCATGGATGTTCCCTGCACAAATGGGCACGGCTCTCATAGACTGTGCATTCTGAGACAGCCGCCTGGTCAGAGGGCGGAGACCCCCAATTCCTCTGTAAGCGGTAACATCCTCTTCCCTGTCCTCAGCGAGGACTGCACTACCACAGAGGAAGGAAATCAGCCTGGAAACATGCTAAACTGCAGCCAGAACTCCAGCTCATCCTCAGTGTGGTGGCTGAAATCACCTGCATTTTCCAGTGGTTCTTCTGAGGGGGACAGCCCTTGGTCCTATCTGAATTCCAGTGGGAGTTCTTGGGTTTCATTGCCGGGAAAGATGAGGAAAGAGATCCTTGAGGCTCGCACCTTGCAACCTGATGACTTTGAAAAGCTGTTGGCAGGAGTGAGGCATGATTGGCTGTTTCAGAGACTAGAGAATACGGGGGTTTTTAAGCCCAGTCAACTCCACCGAGCACATAGTGCTCTTTTGTTAAAATATTCAAAAAAATCTGAACTGTGGACGGCCCAGGAAACTATTGTCTATTTGGGGGACTACTTGACTGTGAAGAAAAAAGGCAGACAAAGAAATGCTTTTTGGGTTCATCATCTTCATCAAGAAGAAATTCTGGGGAGGTATGTTGGGAtgGACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACTGATGTGGAGCGACAGATGACCGCACAGCACTATGTGACAGAATTTAACAAGAGACTCTATGAACAAAACATTCCCACCCAGATATTCTACATCCCATCCACAATACTACTGATTTTAGAGGACAAGACAATAAAGGGATGTATCAGTGTGGAGCCTTACATACTGGGAGAATTTGTAAAATTGTCAAATAACACGAAAGTGGTGAAAACAGAATACAAAGCCACAGAATATGGCTTGGCCTATGGCCATTTTTCTTATGAGTTTTCTAATCATAGAGATGTTGTGGTCGATTTACAAGGTTGGGTAACCGGTAATGGAAAAGGACTCATCTACCTCACAGATCCCCAGATTCACTCCGTTGATCAGAAAGTTTTCACTACCAATTTTGGAAAGAGAGGAATTTTTTACTTCTTTAATAACCAGCATGTGGAATGTAATGAAATCTGCCATCGTCTTTCTTTGACTAGACCTTCAATGGAGAAACCATGCACATAGgcggccgc

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGGMDYKDHDGDYKDHDIDYKDDDDKGSMNNQKVVAVLLQECKQVLDQLLLEAPDVSEEDKSEDQRCRALLPSELRTLIQEAKEMKWPFVPEKWQYKQAVGPEDKTNLKDVIGAGLQQLLASLRASILARDCAAAAAIVFLVDRFLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARISVNSGKLLKAEYILSSLISNNGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASIVGYLALPQPDKKGLSTSLGILADIFVSMSKNDYEKFKNNPQINLSLLKEFDHHLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSSSNDCPPELKNLHLCEAKEAFEIGLLTKRDDEPVTGKQELHSFVKAAFGLTTVHRRLHGETGTVHAASQLCKEAMGKLYNFSTSSRSQDREALSQEVMSVIAQVKEHLQVQSFSNVDDRSYVPESFECRLDKLILHGQGDFQKILDTYSQHHTSVCEVFESDCGNNKNEQKDAKTGVCITALKTEIKNIDTVSTTQEKPHCQRDTGISSSLMGKNVQRELRRGGRRNWTHSDAFRVSLDQDVETETEPSDYSNGEGAVFNKSLSGSQTSSAWSNLSGFSSSASWEEVNYHVDDRSARKEPGKEHLVDTQCSTALSEELENDREGRAMHSLHSQLHDLSLQEPNNDNLEPSQNQPQQQMPLTPFSPHNTPGIFLAPGAGLLEGAPEGIQEVRNMGPRNTSAHSRPSYRSASWSSDSGRPKNMGTHPSVQKEEAFEIIVEFPETNCDVKDRQGKEQGEEISERGAGPTFKASPSWVDPEGETAESTEDAPLDFHRVLHNSLGNISMLPCSSFTPNWPVQNPDSRKSGGPVAEQGIDPDASTVDEEGQLLDSMDVPCTNGHGSHRLCILRQPPGQRAETPNSSVSGNILFPVLSEDCTTTEEGNQPGNMLNCSQNSSSSSVWWLKSPAFSSGSSEGDSPWSYLNSSGSSWVSLPGKMRKEILEARTLQPDDFEKLLAGVRHDWLFQRLENTGVFKPSQLHRAHSALLLKYSKKSELWTAQETIVYLGDYLTVKKKGRQRNAFWVHHLHQEEILGRYVGMDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNIPTQIFYIPSTILLILEDKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKVFTTNFGKRGIFYFFNNQHVECNEICHRLSLTRPSMEKPCT*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT-TO-MBP-3FLAG-ALPK1 K1067M928.11 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65928
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 40934
• Low Temp: No