cDNA Clone NOD1

• Plasmid: pEBG6P-NOD1
• Parent Plasmid: pEBG6P
• Expressed: GST-NOD1
• Genbank: AAH40339
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGAGCAGGGCCACAGTGAGATGGAAATAATCCCATCAGA
GTCTCACCCCCACATTCAATTACTGAAAAGCAATCGGGAACTTCTGGTCA
CTCACATCCGCAATACTCAGTGTCTGGTGGACAACTTGCTGAAGAATGAC
TACTTCTCGGCCGAAGATGCGGAGATTGTGTGTGCCTGCCCCACCCAGCC
TGACAAGGTCCGCAAAATTCTGGACCTGGTACAGAGCAAGGGCGAGGAGG
TGTCCGAGTTCTTCCTCTACTTGCTCCAGCAACTCGCAGATGCCTACGTG
GACCTCAGGCCTTGGCTGCTGGAGATCGGCTTCTCCCCTTCCCTGCTCAC
TCAGAGCAAAGTCGTGGTCAACACTGACCCAGTGAGCAGGTATACCCAGC
AGCTGCGACACCATCTGGGCCGTGACTCCAAGTTCGTGCTGTGCTATGCC
CAGAAGGAGGAGCTGCTGCTGGAGGAGATCTACATGGACACCATCATGGA
GCTGGTTGGCTTCAGCAATGAGAGCCTGGGCAGCCTGAACAGCCTGGCCT
GCCTCCTGGACCACACCACCGGCATCCTCAATGAGCAGGGTGAGACCATC
TTCATCCTGGGTGATGCTGGGGTGGGCAAGTCCATGCTGCTACAGCGGCT
GCAGAGCCTCTGGGCCACGGGCCGGCTAGACGCAGGGGTCAAATTCTTCT
TCCACTTTCGCTGCCGCATGTTCAGCTGCTTCAAGGAAAGTGACAGGCTG
TGTCTGCAGGACCTGCTCTTCAAGCACTACTGCTACCCAGAGCGGGACCC
CGAGGAGGTGTTTGCCTTCCTGCTGCGCTTCCCCCACGTGGCCCTCTTCA
CCTTCGATGGCCTGGACGAGCTGCACTCGGACTTGGACCTGAGCCGCGTG
CCTGACAGCTCCTGCCCCTGGGAGCCTGCCCACCCCCTGGTCTTGCTGGC
CAACCTGCTCAGTGGGAAGCTGCTCAAGGGGGCTAGCAAGCTGCTCACAG
CCCGCACAGGCATCGAGGTCCCGCGCCAGTTCCTGCGGAAGAAGGTGCTT
CTCCGGGGCTTCTCCCCCAGCCACCTGCGCGCCTATGCCAGGAGGATGTT
CCCCGAGCGGGCCCTGCAGGACCGCCTGCTGAGCCAGCTGGAGGCCAACC
CCAACCTCTGCAGCCTGTGCTCTGTGCCCCTCTTCTGCTGGATCATCTTC
CGGTGCTTCCAGCACTTCCGTGCTGCCTTTGAAGGCTCACCACAGCTGCC
CGACTGCACGATGACCCTGACAGATGTCTTCCTCCTGGTCACTGAGGTCC
ATCTGAACAGGATGCAGCCCAGCAGCCTGGTGCAGCGGAACACACGCAGC
CCAGTGGAGACCCTCCACGCCGGCCGGGACACTCTGTGCTCGCTAGGGCA
GGTGGCCCACCGGGGCATGGAGAAGAGCCTCTTTGTCTTCACCCAGGAGG
AGGTGCAGGCCTCCGGGCTGCAGGAGAGAGACATGCAGCTGGGCTTCCTG
CGGGCTTTGCCGGAGCTGGGCCCCGGGGGTGACCAGCAGTCCTATGAGTT
TTTCCACCTCACCCTCCAGGCCTTCTTTACAGCCTTCTTCCTCGTGCTGG
ACGACAGGGTGGGTACTCAGGAGCTGCTCAGGTTCTTCCAGGAGTGGATG
CCCCCTGCGGGGGCAGCGACCACGTCCTGCTATCCTCCCTTCCTCCCGTT
CCAGTGCCTGCAGGGCAGTGGTCCGGCGCGGGAAGACCTCTTCAAGAACA
AGGATCACTTCCAGTTCACCAACCTCTTCCTGTGCGGGCTGTTGTCCAAA
GCCAAACAGAAACTCCTGCGGCATCTGGTGCCCGCGGCAGCCCTGAGGAG
AAAGCGCAAGGCCCTGTGGGCACACCTGTTTTCCAGCCTGCGGGGCTACC
TGAAGAGCCTGCCCCGCGTTCAGGTCGAAAGCTTCAACCAGGTGCAGGCC
ATGCCCACGTTCATCTGGATGCTGCGCTGCATCTACGAGACACAGAGCCA
GAAGGTGGGGCAGCTGGCGGCCAGGGGCATCTGCGCCAACTACCTCAAGC
TGACCTACTGCAACGCCTGCTCGGCCGACTGCAGCGCCCTCTCCTTCGTC
CTGCATCACTTCCCCAAGCGGCTGGCCCTAGACCTAGACAACAACAATCT
CAACGACTACGGCGTGCGGGAGCTGCAGCCCTGCTTCAGCCGCCTCACTG
TTCTCAGACTCAGCGTAAACCAGATCACTGACGGTGGGGTAAAGGTGCTA
AGCGAAGAGCTGACCAAATACAAAATTGTGACCTATTTGGGTTTATACAA
CAACCAGATCACCGATGTCGGAGCCAGGTACGTCACCAAAATCCTGGATG
AATGCAAAGGCCTCACGCATCTTAAACTGGGAAAAAACAAAATAACAAGT
GAAGGAGGGAAGTATCTCGCCCTGGCTGTGAAGAACAGCAAATCAATCTC
TGAGGTTGGGATGTGGGGCAATCAAGTTGGGGATGAAGGAGCAAAAGCCT
TCGCAGAGGCTCTGCGGAACCACCCCAGCTTGACCACCCTGAGTCTTGCG
TCCAACGGCATCTCCACAGAAGGAGGAAAGAGCCTTGCGAGGGCCCTGCA
GCAGAACACGTCTCTAGAAATACTGTGGCTGACCCAAAATGAACTCAACG
ATGAAGTGGCAGAGAGTTTGGCAGAAATGTTGAAAGTCAACCAGACGTTA
AAGCATTTATGGCTTATCCAGAATCAGATCACAGCTAAGGGGACTGCCCA
GCTGGCAGATGCGTTACAGAGCAACACTGGCATAACAGAGATTTGCCTAA
ATGGAAACCTGATAAAACCAGAGGAGGCCAAAGTCTATGAAGATGAGAAG
CGGATTATCTGTTTCTGAGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEEQGHSEMEIIP
SESHPHIQLLKSNRELLVTHIRNTQCLVDNLLKNDYFSAEDAEIVCACPT
QPDKVRKILDLVQSKGEEVSEFFLYLLQQLADAYVDLRPWLLEIGFSPSL
LTQSKVVVNTDPVSRYTQQLRHHLGRDSKFVLCYAQKEELLLEEIYMDTI
MELVGFSNESLGSLNSLACLLDHTTGILNEQGETIFILGDAGVGKSMLLQ
RLQSLWATGRLDAGVKFFFHFRCRMFSCFKESDRLCLQDLLFKHYCYPER
DPEEVFAFLLRFPHVALFTFDGLDELHSDLDLSRVPDSSCPWEPAHPLVL
LANLLSGKLLKGASKLLTARTGIEVPRQFLRKKVLLRGFSPSHLRAYARR
MFPERALQDRLLSQLEANPNLCSLCSVPLFCWIIFRCFQHFRAAFEGSPQ
LPDCTMTLTDVFLLVTEVHLNRMQPSSLVQRNTRSPVETLHAGRDTLCSL
GQVAHRGMEKSLFVFTQEEVQASGLQERDMQLGFLRALPELGPGGDQQSY
EFFHLTLQAFFTAFFLVLDDRVGTQELLRFFQEWMPPAGAATTSCYPPFL
PFQCLQGSGPAREDLFKNKDHFQFTNLFLCGLLSKAKQKLLRHLVPAAAL
RRKRKALWAHLFSSLRGYLKSLPRVQVESFNQVQAMPTFIWMLRCIYETQ
SQKVGQLAARGICANYLKLTYCNACSADCSALSFVLHHFPKRLALDLDNN
NLNDYGVRELQPCFSRLTVLRLSVNQITDGGVKVLSEELTKYKIVTYLGL
YNNQITDVGARYVTKILDECKGLTHLKLGKNKITSEGGKYLALAVKNSKS
ISEVGMWGNQVGDEGAKAFAEALRNHPSLTTLSLASNGISTEGGKSLARA
LQQNTSLEILWLTQNELNDEVAESLAEMLKVNQTLKHLWLIQNQITAKGT
AQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRIICF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3943
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression. Differs to genbank at H447R. H447R is a standard SNP found in all Europeans ; see ; http://www.ensembl.org/Homo_sapiens/snpview?snp=rs2975634&source=dbSNP; Calc Mol Weight : 135022 Daltons