cDNA Clone OSM

• Plasmid: pGEX6P-1-OSM
• Parent Plasmid: pGEX6P1
• Expressed: GST-OSM
• Genbank: NP_113631
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGAGGAGGGCAAGAAGGGCAAGAAGCCTGGAATTGTCTC
GCCATTTAAACGAGTATTCCTAAAAGGTGAAAAGAGTAGAGATAAGAAAG
CCCATGAGAAGGTGACAGAGAGGCGCCCTCTGCACACTGTGGTGTTGTCA
TTGCCTGAGCGCGTCGAGCCAGACAGACTGCTGAGCGACTATATTGAGAA
GGAGGTAAAGTATTTAGGTCAGTTAACGTCCATACCAGGATACCTGAATC
CCTCCAGTAGGACTGAAATCCTGCATTTCATAGACAATGCAAAGAGAGCC
CACCAGCTTCCGGGACACTTGACTCAGGAGCACGATGCTGTGCTCAGCCT
GTCTGCGTACAACGTCAAGCTGGCCTGGAGGGACGGGGAGGATATCATCC
TCAGGGTGCCCATCCATGACATCGCCGCCGTCTCCTATGTTCGGGATGAC
GCTGCACACCTGGTGGTCCTGAAGACAGCCCAGGACCCAGGGATCTCCCC
CAGCCAGAGTCTGTGTGCGGAAAGTTCCAGAGGCCTCAGTGCAGGCTCCC
TGTCGGAGAGTGCAGTTGGGCCCGTGGAGGCATGCTGCCTGGTCATCCTG
GCTGCAGAGAGCAAGGTCGCTGCGGAGGAGCTTTGCTGTCTGCTAGGCCA
GGTCTTCCAGGTTGTTTACACGGAGTCCACCATCGACTTTCTGGACAGAG
CGATATTTGATGGGGCCTCTACCCCGACCCACCACCTGTCCCTGCACAGC
GATGACTCTTCTACAAAAGTGGACATTAAGGAGACCTACGAGGTGGAAGC
CAGCACTTTCTGCTTCCCTGAATCTGTGGATGTGGGTGGTGCATCACCCC
ACAGCAAGACCATCAGTGAGAGCGAGCTGAGCGCCAGCGCCACTGAGCTG
CTGCAGGACTACATGCTGACGCTGCGCACCAAGCTGTCATCACAGGAGAT
CCAGCAGTTTGCAGCACTGCTGCACGAGTACCGCAATGGGGCCTCTATCC
ACGAGTTCTGCATCAACCTGCGGCAGCTCTACGGGGACAGCCGCAAGTTC
CTGCTGCTTGGTCTGAGGCCCTTCATCCCTGAGAAGGACAGCCAGCACTT
CGAGAACTTCCTGGAGACCATTGGCGTGAAGGATGGCCGCGGCATCATCA
CTGACAGCTTTGGCAGGCACCGGCGGGCCCTGAGCACCACATCCAGTTCC
ACCACCAATGGGAACAGGGCCACGGGCAGCTCTGATGACCGGTCGGCACC
CTCAGAGGGGGATGAGTGGGACCGCATGATCTCGGACATCAGCAGCGACA
TTGAGGCGCTGGGCTGCAGCATGGACCAGGACTCAGCATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEEEGKKGKKPGIVSPFKR
VFLKGEKSRDKKAHEKVTERRPLHTVVLSLPERVEPDRLLSDYIEKEVKY
LGQLTSIPGYLNPSSRTEILHFIDNAKRAHQLPGHLTQEHDAVLSLSAYN
VKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDPGISPSQSL
CAESSRGLSAGSLSESAVGPVEACCLVILAAESKVAAEELCCLLGQVFQV
VYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKETYEVEASTFC
FPESVDVGGASPHSKTISESELSASATELLQDYMLTLRTKLSSQEIQQFA
ALLHEYRNGASIHEFCINLRQLYGDSRKFLLLGLRPFIPEKDSQHFENFL
ETIGVKDGRGIITDSFGRHRRALSTTSSSTTNGNRATGSSDDRSAPSEGD
EWDRMISDISSDIEALGCSMDQDSA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3694
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.