cDNA Clone GCK

• Synonyms: MAP4K2
• Plasmid: pEBG6P-GCK corrected
• Parent Plasmid: pEBG6P
• Expressed: GST-GCK
• Genbank: BC047865
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGCGTCGA
CGCGCTGCTGCGGGATGTGTCGCTGCAGGACCCGCGGGACCGCTTCGAGC
TGCTGCAGCGCGTGGGGGCCGGGACCTATGGCGACGTCTACAAGGCCCGC
GACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGTCAAGCTAGACCC
AGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCGTGAGT
GCCGCCACCCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGAATGAC
CGCTTGTGGATCTGCATGGAGTTCTGCGGAGGGGGCTCCCTGCAGGAGAT
TTACCATGCCACTGGGCCCCTGGAGGAGCGGCAGATTGCCTACGTCTGCC
GAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAGATCCAC
AGAGACATCAAGGGAGCCAACCTTCTCCTCACTCTCCAGGGAGATGTCAA
ACTGGCTGACTTTGGGGTGTCAGGCGAGCTGACAGCGTCTGTGGCCAAGA
GGAGGTCTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGGCTGCT
GTGGAGCGCAAAGGTGGCTACAATGAGCTATGTGACGTCTGGGCCCTGGG
CATCACTGCCATTGAGCTGGGCGAGCTGCAGCCCCCTCTGTTCCACCTGC
ACCCCATGAGGGCCCTGATGCTCATGTCGAAGAGCAGCTTCCAGCCGCCC
AAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTTCTCAA
ACTGGCCCTGACCAAGAATCCTAAGAAGAGGCCGACAGCAGAGAAGCTCC
TGCAGCACCCGTTCACGACTCAGCAGCTCCCTCGGGCCCTCCTCACACAG
CTGCTGGACAAAGCCAGTGACCCTCATCTGGGGACCCCCTCCCCTGAGGA
CTGTGAGCTGGAGACCTATGACATGTTTCCAGACACCATTCACTCCCGGG
GGCAGCACGGCCCAGCCGAGAGGACCCCCTCGGAGATCCAGTTTCACCAG
GTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAATGAGCC
GTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGGGAGCC
TGCTGCAGTCGGTCCAGGAGGCCCTGGAGGAAAGGAGTCTGACTATTCGG
TCAGCCTCAGAATTCCAGGAGCTGGACTCCCCAGACGATACCATGGGAAC
CATCAAGCGGGCCCCGTTCCTAGGGCCACTCCCCACTGACCCTCCAGCAG
AGGAGCCTCTGTCCAGTCCCCCAGGCCCCAACAGCTCCCCACTGCTGCCC
ACGGCCTGGGCCACCATGAAGCAGCGGGAGGATCCTGAGAGGTCATCCTG
CCACGGGCTCCCCCCAACTCCCAAGGTGCATATGGGCGCCTGCTTCTCCA
AGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGCTGTCACCTGGATT
CACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAAGGCAT
CTACACACTCAACCTGCATGAACTGCATGAGGATACGCTGGAGAAGCTGA
TTTCACATCGCTGCTCCTGGCTCTACTGCGTGAACAACGTGCTGCTGTCA
CTCTCAGGGAAATCCACGCACATCTGGGCCCATGACCTCCCAGGCCTGTT
TGAGCAGCGGAGGCTACAGCAACAGGTTCCCCTCTCCATCCCCACCAACC
GCCTCACCCAGCGCATCATCCCCAGGCGCTTTGCTCTGTCCACCAAGATT
CCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCCCTACAC
GGGTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGCCTGCTCCTGCTGC
AGTGGTATGAGCCGCTGCAGAAGTTTCTGCTGCTGAAGAACTTCTCCAGC
CCTCTGCCCAGCCCAGCTGGGATGCTGGAGCCGCTGGTGCTGGATGGGAA
GGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGGGGCCTGAGGGGCCCGGCT
GCCGCGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGGCCTGACGCCCGAC
ATCCTCATCCCACCTGAGGGGATCCCAGGCTCGGCCCAGCAGGTGATCCA
GGTGGACAGGGACACAATCCTAGTCAGCTTTGAACGCTGTGTGAGGATTG
TCAACATGCAGGGCGAGCCCACGGCCACACTGGCACCTGAGCTGACCTTT
GATTTCCCCATCGAGACTGTGGTGTGCCTGCAGGACAGTGTGCTGGCCTT
CTGGAGCCATGGGATGCAAGGCCGAAGCCTGGATACCAATGAGGTGACCC
AGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCTTGGGGCCCACAGA
GACATCATCCTGGAGAGCATTCCCACTGACAACCCAGAGGCGCACAGCAA
CCTCTACATCCTCACGGGCCACCAGAGCACCTACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSNGRQCAGIRPC
VDALLRDVSLQDPRDRFELLQRVGAGTYGDVYKARDTVTSELAAVKIVKL
DPGDDISSLQQEITILRECRHPNVVAYIGSYLRNDRLWICMEFCGGGSLQ
EIYHATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGD
VKLADFGVSGELTASVAKRRSFIGTPYWMAPEVAAVERKGGYNELCDVWA
LGITAIELGELQPPLFHLHPMRALMLMSKSSFQPPKLRDKTRWTQNFHHF
LKLALTKNPKKRPTAEKLLQHPFTTQQLPRALLTQLLDKASDPHLGTPSP
EDCELETYDMFPDTIHSRGQHGPAERTPSEIQFHQVKFGAPRRKETDPLN
EPWEEEWTLLGKEELSGSLLQSVQEALEERSLTIRSASEFQELDSPDDTM
GTIKRAPFLGPLPTDPPAEEPLSSPPGPNSSPLLPTAWATMKQREDPERS
SCHGLPPTPKVHMGACFSKVFNGCPLRIHAAVTWIHPVTRDQFLVVGAEE
GIYTLNLHELHEDTLEKLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPG
LFEQRRLQQQVPLSIPTNRLTQRIIPRRFALSTKIPDTKGCLQCRVVRNP
YTGATFLLAALPTSLLLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLD
GKELPQVCVGAEGPEGPGCRVLFHVLPLEAGLTPDILIPPEGIPGSAQQV
IQVDRDTILVSFERCVRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVL
AFWSHGMQGRSLDTNEVTQEITDETRIFRVLGAHRDIILESIPTDNPEAH
SNLYILTGHQSTY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3621
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.