cDNA Clone HSP90

• Plasmid: pEBG6P-HSP90
• Parent Plasmid: pEBG6P
• Expressed: GST-HSP90
• Genbank: AAQ63401
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGCCTGAGGAAGTGCACCATGGAGAGGAGGAGGTGGAGAC
TTTTGCCTTTCAGGCAGAAATTGCCCAACTCATGTCCCTCATCATCAATA
CCTTCTATTCCAACAAGGAGATTTTCCTTCGGGAGTTGATCTCTAATGCT
TCTGATGCCTTGGACAAGATTCGCTATGAGAGCCTGACAGACCCTTCGAA
GTTGGACAGTGGTAAAGAGCTGAAAATTGACATCATCCCCAACCCTCAGG
AACGTACCCTGACTTTGGTAGACACAGGCATTGGCATGACCAAAGCTGAT
CTCATAAATAATTTGGGAACCATTGCCAAGTCTGGTACTAAAGCATTCAT
GGAGGCTCTTCAGGCTGGTGCAGACATCTCCATGATTGGGCAGTTTGGTG
TTGGCTTTTATTCTGCCTACTTGGTGGCAGAGAAAGTGGTTGTGATCACA
AAGCACAACGATGATGAACAGTATGCTTGGGAGTCTTCTGCTGGAGGTTC
CTTCACTGTGCGTGCTGACCATGGTGAGCCCATTGGCAGGGGTACCAAAG
TGATCCTCCATCTTAAAGAAGATCAGACAGAGTACCTAGAAGAGAGGCGG
GTCAAAGAAGTAGTGAAGAAGCATTCTCAGTTCATAGGCTATCCCATCAC
CCTTTATTTGGAGAAGGAACGAGAGAAGGAAATTAGTGATGATGAGGCAG
AGGAAGAGAAAGGTGAGAAAGAAGAGGAAGATAAAGATGATGAAGAAAAG
CCCAAGATCGAAGATGTGGGTTCAGATGAGGAGGATGACAGCGGTAAGGA
TAAGAAGAAGAAAACTAAGAAGATCAAAGAGAAATACATTGATCAGGAAG
AACTAAACAAGACCAAGCCTATTTGGACCAGAAACCCTGATGACATCACC
CAAGAGGAGTATGGAGAATTCTACAAGAGCCTCACTAATGACTGGGAAGA
CCACTTGGCAGTCAAGCACTTTTCTGTAGAAGGTCAGTTGGAATTCAGGG
CATTGCTATTTATTCCTCGTCGGGCTCCCTTTGACCTTTTTGAGAACAAG
AAGAAAAAGAACAACATCAAACTCTATGTCCGCCGTGTGTTCATCATGGA
CAGCTGTGATGAGTTGATACCAGAGTATCTCAATTTTATCCGTGGTGTGG
TTGACTCTGAGGATCTGCCCCTGAACATCTCCCGAGAAATGCTCCAGCAG
AGCAAAATCTTGAAAGTCATTCGCAAAAACATTGTTAAGAAGTGCCTTGA
GCTCTTCTCTGAGCTGGCAGAAGACAAGGAGAATTACAAGAAATCCTATG
AGGCATTCTCTAAAAATCTCAAGCTTGGAATCCACGAAGACTCCACTAAC
CGCCGCCGCCTGTCTGAGCTGCTGCGCTATCATACCTCCCAGTCTGGAGA
TGAGATGACATCTCTGTCAGAGTATGTTTCTCGCATGAAGGAGACACAGA
AGTCCATCTATTACATCACTGGTGAGAGCAAAGAGCAGGTGGCCAACTCA
GCTTTTGTGGAGCGAGTGCGGAAACGGGGCCTCGAGGTGGTATATATGAC
CGAGCCCATTGACGAGTACTGTGTGCAGCAGCTCAAGGAATTTGATGGGA
AGAGCCTGGTCTCAGTTACCAAGGAGGGTCTGGAGCTGCCTGAGGATGAG
GAGGAGAAGAAGAAGATGGAAGAGAGCAAGGCAAAGTTTGAGAACCTCTG
CAAGCTCATGAAAGAAATCTTAGATAAGAAGGTTGAGAAGGTGACAATCT
CCAATAGACTTGTGTCTTCACCTTGCTGCATTGTGACCAGCACCTACGGC
TGGACAGCCAATATGGAGCGGATCATGAAAGCCCAGGCACTTCGGGACAA
CTCCACCATGGGCTATATGATGGCCAAAAAGCACCTGGAGATCAACCCTG
ACCACCCCATTGTGGAGACGCTGCGGCAGAAGGCTGAGGCCGACAAGAAT
GATAAGGCAGTTAAGGACCTGGTGGTGCTGCTGTTTGAAACCGCCCTGCT
ATCTTCTGGCTTTTCCCTTGAGGATCCCCAGACCCACTCCAACCGCATCT
ATCGCATGATCAAGCTAGGTCTAGGTATTGATGAAGATGAAGTGGCAGCA
GAGGAACCCAATGCTGCAGTTCCTGATGAGATCCCCCCTCTCGAGGGCGA
TGAGGATGCGTCTCGCATGGAAGAAGTCGATTAGGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMPEE
VHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKI
RYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGT
IAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQ
YAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKK
HSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVG
SDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEF
YKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIK
LYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVI
RKNIVKKCLELFSELAEDKENYKKSYEAFSKNLKLGIHEDSTNRRRLSEL
LRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVR
KRGLEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKME
ESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMER
IMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDL
VVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAV
PDEIPPLEGDEDASRMEEVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3615
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Calc Mol WEight : 111372 Daltons