cDNA Clone SEF

• Plasmid: pEBG6P-SEF 268-706
• Parent Plasmid: pEBG6P
• Expressed: GST-SEF 268-706
• Genbank: BC038369
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCATCACAGTGCCACTGGTAGTCATATCGGCATTCGC
GACGCTCTTCACTGTGATGTGCCGCAAGAAGCAACAAGAAAATATATATT
CACATTTAGATGAAGAGAGCTCTGAGTCTTCCACATACACTGCAGCACTC
CCAAGAGAGAGGCTCCGGCCGCGGCCGAAGGTCTTTCTCTGCTATTCCAG
TAAAGATGGCCAGAATCACATGAATGTCGTCCAGTGTTTCGCCTACTTCC
TCCAGGACTTCTGTGGCTGTGAGGTGGCTCTGGACCTGTGGGAAGACTTC
AGCCTCTGTAGAGAAGGGCAGAGAGAATGGGTCATCCAGAAGATCCACGA
GTCCCAGTTCATCATTGTGGTTTGTTCCAAAGGTATGAAGTACTTTGTGG
ACAAGAAGAACTACAAACACAAAGGAGGTGGCCGAGGCTCGGGGAAAGGA
GAGCTCTTCCTGGTGGCAGTGTCAGCCATTGCCGAAAAGCTCCGCCAGGC
CAAGCAGAGTTCGTCCGCGGCGCTCAGCAAGTTTATCGCCGTCTACTTTG
ATTATTCCTGCGAGGGAGACGTCCCCGGTATCCTAGACCTGAGTACCAAG
TACAGACTCATGGACAATCTTCCTCAGCTCTGTTCCCACCTGCACTCCCG
AGACCACGGCCTCCAGGAGCCGGGGCAGCACACGCGACAGGGCAGCAGAA
GGAACTACTTCCGGAGCAAGTCAGGCCGGTCCCTATACGTCGCCATTTGC
AACATGCACCAGTTTATTGACGAGGAGCCCGACTGGTTCGAAAAGCAGTT
CGTTCCCTTCCATCCTCCTCCACTGCGCTACCGGGAGCCAGTCTTGGAGA
AATTTGATTCGGGCTTGGTTTTAAATGATGTCATGTGCAAACCAGGGCCT
GAGAGTGACTTCTGCCTAAAGGTAGAGGCGGCTGTTCTTGGGGCAACCGG
ACCAGCCGACTCCCAGCACGAGAGTCAGCATGGGGGCCTGGACCAAGACG
GGGAGGCCCGGCCTGCCCTTGACGGTAGCGCCGCCCTGCAACCCCTGCTG
CACACGGTGAAAGCCGGCAGCCCCTCGGACATGCCGCGGGACTCAGGCAT
CTATGACTCGTCTGTGCCCTCATCCGAGCTGTCTCTGCCACTGATGGAAG
GACTCTCGACGGACCAGACAGAAACGTCTTCCCTGACGGAGAGCGTGTCC
TCCTCTTCAGGCCTGGGTGAGGAGGAACCTCCTGCCCTTCCTTCCAAGCT
CCTCTCTTCTGGGTCATGCAAAGCAGATCTTGGTTGCCGCAGCTACACTG
ATGAACTCCACGCGGTCGCCCCTTTGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAIT
VPLVVISAFATLFTVMCRKKQQENIYSHLDEESSESSTYTAALPRERLRP
RPKVFLCYSSKDGQNHMNVVQCFAYFLQDFCGCEVALDLWEDFSLCREGQ
REWVIQKIHESQFIIVVCSKGMKYFVDKKNYKHKGGGRGSGKGELFLVAV
SAIAEKLRQAKQSSSAALSKFIAVYFDYSCEGDVPGILDLSTKYRLMDNL
PQLCSHLHSRDHGLQEPGQHTRQGSRRNYFRSKSGRSLYVAICNMHQFID
EEPDWFEKQFVPFHPPPLRYREPVLEKFDSGLVLNDVMCKPGPESDFCLK
VEAAVLGATGPADSQHESQHGGLDQDGEARPALDGSAALQPLLHTVKAGS
PSDMPRDSGIYDSSVPSSELSLPLMEGLSTDQTETSSLTESVSSSSGLGE
EEPPALPSKLLSSGSCKADLGCRSYTDELHAVAPL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3518
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.