cDNA Clone Hip-55

• Plasmid: pGEX6P-1-HIP-55
• Parent Plasmid: pGEX6P1
• Expressed: GST-HIP-55
• Genbank: NM_014063
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGAACCTGAGCCGGAACGGGCCAGCGCTGCAAGAGGC
CTACGTGCGGGTGGTCACCGAGAAGTCCCCGACCGACTGGGCTCTCTTTA
CCTATGAAGGCAACAGCAATGACATCCGCGTGGCTGGCACAGGGGAGGGT
GGCCTGGAGGAGATGGTGGAGGAGCTCAACAGCGGGAAGGTGATGTACGC
CTTCTGCAGAGTGAAGGACCCCAACTCTGGACTGCCCAAATTTGTCCTCA
TCAACTGGACAGGCGAGGGCGTGAACGATGTGCGGAAGGGAGCCTGTGCC
AGCCACGTCAGCACCATGGCCAGCTTCCTGAAGGGGGCCCATGTGACCAT
CAACGCACGGGCCGAGGAGGATGTGGAGCCTGAGTGCATCATGGAGAAGG
TGGCCAAGGCTTCAGGTGCCAACTACAGCTTTCACAAGGAGAGTGGCCGC
TTCCAGGACGTGGGACCCCAGGCCCCAGTGGGCTCTGTGTACCAGAAGAC
CAATGCCGTGTCTGAGATTAAAAGGGTTGGTAAAGACAGCTTCTGGGCCA
AAGCAGAGAAGGAGGAGGAGAACCGTCGGCTGGAGGAAAAGCGGCGGGCC
GAGGAGGCACAGCGGCAGCTGGAGCAGGAGCGCCGGGAGCGTGAGCTGCG
TGAGGCTGCACGCCGGGAGCAGCGCTATCAGGAGCAGGGTGGCGAGGCCA
GCCCCCAGAGCAGGACGTGGGAGCAGCAGCAAGAAGTGGTTTCAAGGAAC
CGAAATGAGCAGGAGTCTGCCGTGCACCCGAGGGAGATTTTCAAGCAGAA
GGAGAGGGCCATGTCCACCACCTCCATCTCCAGTCCTCAGCCTGGCAAGC
TGAGGAGCCCCTTCCTGCAGAAGCAGCTCACCCAACCAGAGACCCACTTT
GGCAGAGAGCCAGCTGCTGCCATCTCAAGGCCCAGGGCAGATCTCCCTGC
TGAGGAGCCGGCGCCCAGCACTCCTCCATGTCTGGTGCAGGCAGAAGAGG
AGGCTGTGTATGAGGAACCTCCAGAGCAGGAGACCTTCTACGAGCAGCCC
CCACTGGTGCAGCAGCAAGGTGCTGGCTCTGAGCACATTGACCACCACAT
TCAGGGCCAGGGGCTCAGTGGGCAAGGGCTCTGTGCCCGTGCCCTGTACG
ACTACCAGGCAGCCGACGACACAGAGATCTCCTTTGACCCCGAGAACCTC
ATCACGGGCATCGAGGTGATCGACGAAGGCTGGTGGCGTGGCTATGGGCC
GGATGGCCATTTTGGCATGTTCCCTGCCAACTACGTGGAGCTCATTGAGT
GAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAANLSRNGPALQEAYVRV
VTEKSPTDWALFTYEGNSNDIRVAGTGEGGLEEMVEELNSGKVMYAFCRV
KDPNSGLPKFVLINWTGEGVNDVRKGACASHVSTMASFLKGAHVTINARA
EEDVEPECIMEKVAKASGANYSFHKESGRFQDVGPQAPVGSVYQKTNAVS
EIKRVGKDSFWAKAEKEEENRRLEEKRRAEEAQRQLEQERRERELREAAR
REQRYQEQGGEASPQSRTWEQQQEVVSRNRNEQESAVHPREIFKQKERAM
STTSISSPQPGKLRSPFLQKQLTQPETHFGREPAAAISRPRADLPAEEPA
PSTPPCLVQAEEEAVYEEPPEQETFYEQPPLVQQQGAGSEHIDHHIQGQG
LSGQGLCARALYDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHF
GMFPANYVELIE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3439
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST fusion for bacterial expression.