cDNA Clone CHREBP

• Plasmid: pCMV3a CHREBP
• Parent Plasmid: pCMV tag3a
• Expressed: Myc-CHREBP
• Genbank: AF245470
• Tag: Myc
• Species: Human

Sequence of Insert

GGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGCCGGCG
CGCTGGCAGGTCTGGCCGCGGGCTTGCAGGTCCCGCGGGTCGCGCCCAGC
CCAGACTCGGACTCGGACACAGACTCGGAGGACCCGAGTCTCCGGCGCAG
CGCGGGCGGCTTGCTCCGCTCGCAGGTCATCCACAGCGGTCACTTCATGG
TGTCGTCGCCGCACAGCGACTCGCTGCCCCGGCGGCGCGACCAGGAGGGG
TCCGTGGGGCCCTCCGACTTCGGGCCGCGCAGTATCGACCCCACACTCAC
ACGCCTCTTCGAGTGCTTGAGCCTGGCCTACAGTGGCAAGCTGGTGTCTC
CCAAGTGGAAGAATTTCAAAGGCCTCAAGCTGCTCTGCAGAGACAAGATC
CGCCTGAACAACGCCATCTGGAGGGCCTGGTATATCCAGTATGTGAAGCG
GAGGAAGAGCCCCGTGTGTGGCTTCGTGACCCCCCTGCAGGGGCCTGAGG
CTGATGCGCACCGGAAGCCGGAGGCCGTGGTCCTGGAGGGGAACTACTGG
AAGCGGCGCATCGAGGTGGTGATGCGGGAATACCACAAGTGGCGCATCTA
CTACAAGAAGCGGCTCCGTAAGCCCAGCAGGGAAGATGACCTCCTGGCCC
CTAAGCAGGCGGAAGGCAGGTGGCCGCCGCCGGAGCAATGGTGCAAACAG
CTCTTCTCCAGTGTGGTCCCCGTGCTGCTGGGGGACCCAGAGGAGGAGCC
GGGTGGGCGGCAGCCCCTGGACCTCAATTGCTTTTTGTCCGACATCTCGG
ACACTCTCTTCACCATGACTCAGTCCGGCCCTTCGCCCCTGCAGCTGCCG
CCTGAGGATGCCTACGTCGGCAATGCTGACATGATCCAGCCGGACCTGAC
GCCACTGCAGCCAAGCCTGGATGACTTCATGGACATCTCAGATTTCTTTA
CCAACTCCCGCCTCCCACAGCCGCCCATGCCTTCAAACTTCCCAGAGCCC
CCCAGCTTCAGCCCCGTGGTTGACTCCCTCTTCAGCAGTGGGACCCTGGG
CCCAGAGGTGCCCCCGGCTTCCTCGGCCATGACCCACCTCTCTGGACACA
GCCGTCTGCAGGCTCGGAACAGCTGCCCTGGCCCCTTGGACTCCAGCGCC
TTCCTGAGTTCTGATTTCCTCCTTCCTGAAGACCCCAAGCCCCGGCTCCC
ACCCCCTCCTGTACCCCCACCTCTGCTGCATTACCCTCCCCCTGCCAAGG
TGCCAGGCCTGGAGCCCTGCCCCCCACCTCCCTTCCCTCCCATGGCACCA
CCCACTGCTTTGCTGCAGGAAGAGCCTCTCTTCTCTCCCAGGTTTCCCTT
CCCCACCGTCCCTCCTGCCCCAGGAGTGTCTCCGCTGCCTGCTCCTGCAG
CCTTCCCACCCACCCCACAGTCTGTCCCCAGCCCAGCCCCCACCCCCTTC
CCCATAGAGCTTCTACCCTTGGGGTATTCGGAGCCTGCCTTTGGGCCTTG
CTTCTCCATGCCCAGAGGCAAGCCCCCCGCCCCATCCCCTAGGGGACAGA
AAGCCAGCCCCCCTACCTTAGCCCCTGCCACTGCCAGTCCCCCCACCACT
GCGGGGAGCAACAACCCCTGCCTCACACAGCTGCTCACAGCAGCTAAGCC
GGAGCAAGCCCTGGAGCCACCACTTGTATCCAGCACCCTCCTCCGGTCCC
CAGGGTCCCCGCAGGAGACAGTCCCTGAATTCCCCTGCACATTCCTTCCC
CCGACCCCGGCCCCTACACCGCCCCGGCCACCTCCAGGCCCGGCCACATT
GGCCCCTTCCAGGCCCCTGCTTGTCCCCAAAGCGGAGCGGCTCTCACCCC
CAGCGCCCAGCGGCAGTGAACGGCGGCTGTCAGGGGACCTCAGCTCCATG
CCAGGCCCTGGGACTCTGAGCGTCCGTGTCTCTCCCCCGCAACCCATCCT
CAGCCGGGGCCGTCCAGACAGCAACAAGACCGAGAACCGGCGTATCACAC
ACATCTCCGCGGAGCAGAAGCGGCGCTTCAACATCAAGCTGGGGTTTGAC
ACCCTTCATGGGCTCGTGAGCACACTCAGTGCCCAGCCCAGCCTCAAGGT
GAGCAAAGCTACCACGCTGCAGAAGACAGCTGAGTACATCCTTATGCTAC
AGCAGGAGCGTGCGGGCTTGCAGGAGGAGGCCCAGCAGCTGCGGGATGAG
ATTGAGGAGCTCAATGCCGCCATTAACCTGTGCCAGCAGCAGCTGCCCGC
CACAGGGGTACCCATCACACACCAGCGTTTTGACCAGATGCGAGACATGT
TTGATGACTACGTCCGAACCCGTACGCTGCACAACTGGAAGTTCTGGGTG
TTCAGCATCCTCATCCGGCCTCTGTTTGAGTCCTTCAACGGGATGGTGTC
CACGGCAAGTGTGCACACCCTCCGCCAGACCTCACTGGCCTGGCTGGACC
AGTACTGCTCTCTGCCCGCTCTCCGGCCAACTGTCCTGAACTCCCTACGC
CAGCTGGGCACATCTACCAGTATCCTGACCGACCCGGGCCGCATCCCTGA
GCAAGCCACACGGGCAGTCACAGAGGGCACCCTTGGCAAACCTTTATAGA
AGGGCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAG

Amino Acid Sequence

MEQKLISEARADPLVTAASVLEFALAGALAGLAAGLQVPRVAPSPDSDSD
TDSEDPSLRRSAGGLLRSQVIHSGHFMVSSPHSDSLPRRRDQEGSVGPSD
FGPRSIDPTLTRLFECLSLAYSGKLVSPKWKNFKGLKLLCRDKIRLNNAI
WRAWYIQYVKRRKSPVCGFVTPLQGPEADAHRKPEAVVLEGNYWKRRIEV
VMREYHKWRIYYKKRLRKPSREDDLLAPKQAEGRWPPPEQWCKQLFSSVV
PVLLGDPEEEPGGRQPLDLNCFLSDISDTLFTMTQSGPSPLQLPPEDAYV
GNADMIQPDLTPLQPSLDDFMDISDFFTNSRLPQPPMPSNFPEPPSFSPV
VDSLFSSGTLGPEVPPASSAMTHLSGHSRLQARNSCPGPLDSSAFLSSDF
LLPEDPKPRLPPPPVPPPLLHYPPPAKVPGLEPCPPPPFPPMAPPTALLQ
EEPLFSPRFPFPTVPPAPGVSPLPAPAAFPPTPQSVPSPAPTPFPIELLP
LGYSEPAFGPCFSMPRGKPPAPSPRGQKASPPTLAPATASPPTTAGSNNP
CLTQLLTAAKPEQALEPPLVSSTLLRSPGSPQETVPEFPCTFLPPTPAPT
PPRPPPGPATLAPSRPLLVPKAERLSPPAPSGSERRLSGDLSSMPGPGTL
SVRVSPPQPILSRGRPDSNKTENRRITHISAEQKRRFNIKLGFDTLHGLV
STLSAQPSLKVSKATTLQKTAEYILMLQQERAGLQEEAQQLRDEIEELNA
AINLCQQQLPATGVPITHQRFDQMRDMFDDYVRTRTLHNWKFWVFSILIR
PLFESFNGMVSTASVHTLRQTSLAWLDQYCSLPALRPTVLNSLRQLGTST
SILTDPGRIPEQATRAVTEGTLGKPL*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU3368
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal Myc tag for mammalian expression