cDNA Clone HGS

• Plasmid: pFBHTb HGS
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-HGS
• Genbank: AAP88756
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGGGCGAGGCAGCGGCACCTTCGAGCGTCTCCTAGACAAGGCGAC
CAGCCAGCTCCTGTTGGAGACAGATTGGGAGTCCATTTTGCAGATCTGCG
ACCTGATCCGCCAAGGGGACACACAAGCAAAATATGCTGTGAATTCCATC
AAGAAGAAAGTCAACGACAAGAACCCACACGTCGCCTTGTATGCCCTGGA
GGTCATGGAATCTGTGGTAAAGAACTGTGGCCAGACAGTTCATGATGAGG
TGGCCAACAAGCAGACCATGGAGGAGCTGAAGGACCTGCTGAAGAGACAA
GTGGAGGTAAACGTCCGTAACAAGATCCTGTACCTGATCCAGGCCTGGGC
GCATGCCTTCCGGAACGAGCCCAAGTACAAGGTGGTCCAGGACACCTACC
AGATCATGAAGGTGGAGGGGCACGTCTTTCCAGAATTCAAAGAGAGCGAT
GCCATGTTTGCTGCCGAGAGAGCCCCAGACTGGGTGGACGCTGAGGAATG
CCACCGCTGCAGGGTGCAGTTCGGGGTGATGACCCGTAAGCACCACTGCC
GGGCGTGTGGGCAGATATTCTGTGGAAAGTGTTCTTCCAAGTACTCCACC
ATCCCCAAGTTTGGCATCGAGAAGGAGGTGCGCGTGTGTGAGCCCTGCTA
CGAGCAGCTGAACAGGAAAGCGGAGGGAAAGGCCACTTCCACCACTGAGC
TGCCCCCCGAGTACCTGACCAGCCCCCTGTCTCAGCAGTCCCAGCTGCCC
CCCAAGAGGGACGAGACGGCCCTGCAGGAGGAGGAGGAGCTGCAGCTGGC
CCTGGCGCTGTCACAGTCAGAGGCGGAGGAGAAGGAGAGGCTGAGACAGA
AGTCCACGTACACTTCGTACCCCAAGGCGGAGCCCATGCCCTCGGCCTCC
TCAGCGCCCCCCGCCAGCAGCCTGTACTCTTCACCTGTGAACTCGTCGGC
GCCTCTGGCTGAGGACATCGACCCTGAGCTCGCACGGTATCTCAACCGGA
ACTACTGGGAGAAGAAGCAGGAGGAGGCTCGCAAGAGCCCCACGCCATCT
GCGCCCGTGCCCCTGACGGAGCCGGCTGCACAGCCTGGGGAAGGGCACGC
AGCCCCCACCAACGTGGTGGAGAACCCCCTCCCGGAGACAGACTCTCAGC
CCATTCCTCCCTCTGGTGGCCCCTTTAGTGAGCCACAGTTCCACAATGGC
GAGTCTGAGGAGAGCCACGAGCAGTTCCTGAAGGCGCTGCAGAACGCCGT
CACCACCTTCGTGAACCGCATGAAGAGTAACCACATGCGGGGCCGCAGCA
TCACCAATGACTCGGCCGTGCTCTCACTCTTCCAGTCCATCAACGGCATG
CACCCGCAGCTGCTGGAGCTGCTCAACCAGCTGGACGAGCGCAGGCTGTA
CTATGAGGGGCTGCAGGACAAGCTGGCACAGATCCGCGATGCCCGGGGGG
CGCTGAGTGCCCTGCGCGAAGAGCACCGGGAGAAGCTTCGCCGGGCAGCC
GAGGAGGCAGAGCGCCAGCGCCAGATCCAGCTGGCCCAGAAGCTGGAGAT
AATGCGGCAGAAGAAGCAGGAGTACCTGGAGGTGCAGAGGCAGCTGGCCA
TCCAGCGCCTGCAGGAGCAGGAGAAGGAGCGGCAGATGCGGCTGGAGCAG
CAGAAGCAGACGGTCCAGATGCGCGCGCAGATGCCCGCCTTCCCCCTGCC
CTACGCCCAGCTCCAGGCCATGCCCGCAGCCGGAGGTGTGCTCTACCAGC
CCTCGGGACCAGCCAGCTTCCCCAGCACCTTCAGCCCTGCCGGCTCGGTG
GAGGGCTCCCCAATGCACGGCGTGTACATGAGCCAGCCGGCCCCTGCCGC
TGGCCCCTACCCCAGCATGCCCAGCACTGCGGCTGATCCCAGCATGGTGA
GTGCCTACATGTACCCAGCAGGGGCCACTGGGGCGCAGGCGGCCCCCCAG
GCCCAGGCCGGACCCACCGCCAGCCCCGCTTACTCATCCTACCAGCCTAC
TCCCACAGCGGGCTACCAGAACGTGGCCTCCCAGGCCCCACAGAGCCTCC
CGGCCATCTCTCAGCCTCCGCAGTCCAGCACCATGGGCTACATGGGGAGC
CAGTCAGTCTCCATGGGCTACCAGCCTTACAACATGCAGAATCTCATGAC
CACCCTCCCAAGCCAGGATGCGTCTCTGCCACCCCAGCAGCCCTACATCG
CGGGGCAGCAGCCCATGTACCAGCAGATGGCACCCTCTGGCGGTCCCCCC
CAGCAGCAGCCCCCCGTGGCCCAGCAACCGCAGGCACAGGGGCCGCCGGC
ACAGGGCAGCGAGGCCCAGCTCATTTCATTCGACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGRGSGTFERLLDKATSQLLLET
DWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVK
NCGQTVHDEVANKQTMEELKDLLKRQVEVNVRNKILYLIQAWAHAFRNEP
KYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQF
GVMTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNRKA
EGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSE
AEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDID
PELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVE
NPLPETDSQPIPPSGGPFSEPQFHNGESEESHEQFLKALQNAVTTFVNRM
KSNHMRGRSITNDSAVLSLFQSINGMHPQLLELLNQLDERRLYYEGLQDK
LAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQE
YLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAM
PAAGGVLYQPSGPASFPSTFSPAGSVEGSPMHGVYMSQPAPAAGPYPSMP
STAADPSMVSAYMYPAGATGAQAAPQAQAGPTASPAYSSYQPTPTAGYQN
VASQAPQSLPAISQPPQSSTMGYMGSQSVSMGYQPYNMQNLMTTLPSQDA
SLPPQQPYIAGQQPMYQQMAPSGGPPQQQPPVAQQPQAQGPPAQGSEAQL
ISFD

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3282
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for baculovirus expression.