cDNA Clone HGS

• Plasmid: pCMV6P-1 HGS
• Parent Plasmid: pCMV5 6P1
• Expressed: pCMV6P-1 HGS
• Genbank: AAP88756
• Species: Human

Sequence of Insert

GGATCCGGGCGAGGCAGCGGCACCTTCGAGCGTCTCCTAGACAAGGCGAC
CAGCCAGCTCCTGTTGGAGACAGATTGGGAGTCCATTTTGCAGATCTGCG
ACCTGATCCGCCAAGGGGACACACAAGCAAAATATGCTGTGAATTCCATC
AAGAAGAAAGTCAACGACAAGAACCCACACGTCGCCTTGTATGCCCTGGA
GGTCATGGAATCTGTGGTAAAGAACTGTGGCCAGACAGTTCATGATGAGG
TGGCCAACAAGCAGACCATGGAGGAGCTGAAGGACCTGCTGAAGAGACAA
GTGGAGGTAAACGTCCGTAACAAGATCCTGTACCTGATCCAGGCCTGGGC
GCATGCCTTCCGGAACGAGCCCAAGTACAAGGTGGTCCAGGACACCTACC
AGATCATGAAGGTGGAGGGGCACGTCTTTCCAGAATTCAAAGAGAGCGAT
GCCATGTTTGCTGCCGAGAGAGCCCCAGACTGGGTGGACGCTGAGGAATG
CCACCGCTGCAGGGTGCAGTTCGGGGTGATGACCCGTAAGCACCACTGCC
GGGCGTGTGGGCAGATATTCTGTGGAAAGTGTTCTTCCAAGTACTCCACC
ATCCCCAAGTTTGGCATCGAGAAGGAGGTGCGCGTGTGTGAGCCCTGCTA
CGAGCAGCTGAACAGGAAAGCGGAGGGAAAGGCCACTTCCACCACTGAGC
TGCCCCCCGAGTACCTGACCAGCCCCCTGTCTCAGCAGTCCCAGCTGCCC
CCCAAGAGGGACGAGACGGCCCTGCAGGAGGAGGAGGAGCTGCAGCTGGC
CCTGGCGCTGTCACAGTCAGAGGCGGAGGAGAAGGAGAGGCTGAGACAGA
AGTCCACGTACACTTCGTACCCCAAGGCGGAGCCCATGCCCTCGGCCTCC
TCAGCGCCCCCCGCCAGCAGCCTGTACTCTTCACCTGTGAACTCGTCGGC
GCCTCTGGCTGAGGACATCGACCCTGAGCTCGCACGGTATCTCAACCGGA
ACTACTGGGAGAAGAAGCAGGAGGAGGCTCGCAAGAGCCCCACGCCATCT
GCGCCCGTGCCCCTGACGGAGCCGGCTGCACAGCCTGGGGAAGGGCACGC
AGCCCCCACCAACGTGGTGGAGAACCCCCTCCCGGAGACAGACTCTCAGC
CCATTCCTCCCTCTGGTGGCCCCTTTAGTGAGCCACAGTTCCACAATGGC
GAGTCTGAGGAGAGCCACGAGCAGTTCCTGAAGGCGCTGCAGAACGCCGT
CACCACCTTCGTGAACCGCATGAAGAGTAACCACATGCGGGGCCGCAGCA
TCACCAATGACTCGGCCGTGCTCTCACTCTTCCAGTCCATCAACGGCATG
CACCCGCAGCTGCTGGAGCTGCTCAACCAGCTGGACGAGCGCAGGCTGTA
CTATGAGGGGCTGCAGGACAAGCTGGCACAGATCCGCGATGCCCGGGGGG
CGCTGAGTGCCCTGCGCGAAGAGCACCGGGAGAAGCTTCGCCGGGCAGCC
GAGGAGGCAGAGCGCCAGCGCCAGATCCAGCTGGCCCAGAAGCTGGAGAT
AATGCGGCAGAAGAAGCAGGAGTACCTGGAGGTGCAGAGGCAGCTGGCCA
TCCAGCGCCTGCAGGAGCAGGAGAAGGAGCGGCAGATGCGGCTGGAGCAG
CAGAAGCAGACGGTCCAGATGCGCGCGCAGATGCCCGCCTTCCCCCTGCC
CTACGCCCAGCTCCAGGCCATGCCCGCAGCCGGAGGTGTGCTCTACCAGC
CCTCGGGACCAGCCAGCTTCCCCAGCACCTTCAGCCCTGCCGGCTCGGTG
GAGGGCTCCCCAATGCACGGCGTGTACATGAGCCAGCCGGCCCCTGCCGC
TGGCCCCTACCCCAGCATGCCCAGCACTGCGGCTGATCCCAGCATGGTGA
GTGCCTACATGTACCCAGCAGGGGCCACTGGGGCGCAGGCGGCCCCCCAG
GCCCAGGCCGGACCCACCGCCAGCCCCGCTTACTCATCCTACCAGCCTAC
TCCCACAGCGGGCTACCAGAACGTGGCCTCCCAGGCCCCACAGAGCCTCC
CGGCCATCTCTCAGCCTCCGCAGTCCAGCACCATGGGCTACATGGGGAGC
CAGTCAGTCTCCATGGGCTACCAGCCTTACAACATGCAGAATCTCATGAC
CACCCTCCCAAGCCAGGATGCGTCTCTGCCACCCCAGCAGCCCTACATCG
CGGGGCAGCAGCCCATGTACCAGCAGATGGCACCCTCTGGCGGTCCCCCC
CAGCAGCAGCCCCCCGTGGCCCAGCAACCGCAGGCACAGGGGCCGCCGGC
ACAGGGCAGCGAGGCCCAGCTCATTTCATTCGACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGRGSGTFERLLDKATSQLL
LETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMES
VVKNCGQTVHDEVANKQTMEELKDLLKRQVEVNVRNKILYLIQAWAHAFR
NEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCR
VQFGVMTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLN
RKAEGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALS
QSEAEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAE
DIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTN
VVENPLPETDSQPIPPSGGPFSEPQFHNGESEESHEQFLKALQNAVTTFV
NRMKSNHMRGRSITNDSAVLSLFQSINGMHPQLLELLNQLDERRLYYEGL
QDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQK
KQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQL
QAMPAAGGVLYQPSGPASFPSTFSPAGSVEGSPMHGVYMSQPAPAAGPYP
SMPSTAADPSMVSAYMYPAGATGAQAAPQAQAGPTASPAYSSYQPTPTAG
YQNVASQAPQSLPAISQPPQSSTMGYMGSQSVSMGYQPYNMQNLMTTLPS
QDASLPPQQPYIAGQQPMYQQMAPSGGPPQQQPPVAQQPQAQGPPAQGSE
AQLISFD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3275
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for mammalian expression.