cDNA Clone IKK beta

• Plasmid: pFB IKK beta
• Parent Plasmid: pFastbac1 mod
• Expressed: 6His-IKK beta
• Genbank: AF080158 (gi 14738764)
• Tag: 6His
• Species: Human

Sequence of Insert

CATATGAGCTGGTCACCTTCCCTGACAACGCAGACATGTGGGGCCTGGGA
AATGAAAGAGCGCCTTGGGACAGGGGGATTTGGAAATGTCATCCGATGGC
ACAATCAGGAAACAGGTGAGCAGATTGCCATCAAGCAGTGCCGGCAGGAG
CTCAGCCCCCGGAACCGAGAGCGGTGGTGCCTGGAGATCCAGATCATGAG
AAGGCTGACCCACCCCAATGTGGTGGCTGCCCGAGATGTCCCTGAGGGGA
TGCAGAACTTGGCGCCCAATGACCTGCCCCTGCTGGCCATGGAGTACTGC
CAAGGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGAACTGCTGTGG
TCTGCGGGAAGGTGCCATCCTCACCTTGCTGAGTGACATTGCCTCTGCGC
TTAGATACCTTCATGAAAACAGAATCATCCATCGGGATCTAAAGCCAGAA
AACATCGTCCTGCAGCAAGGAGAACAGAGGTTAATACACAAAATTATTGA
CCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTTTGCACATCATTCG
TGGGGACCCTGCAGTACCTGGCCCCAGAGCTACTGGAGCAGCAGAAGTAC
ACAGTGACCGTCGACTACTGGAGCTTCGGCACCCTGGCCTTTGAGTGCAT
CACGGGCTTCCGGCCCTTCCTCCCCAACTGGCAGCCCGTGCAGTGGCATT
CAAAAGTGCGGCAGAAGAGTGAGGTGGACATTGTTGTTAGCGAAGACTTG
AATGGAACGGTGAAGTTTTCAAGCTCTTTACCCTACCCCAATAATCTTAA
CAGTGTCCTGTCTGAGCGACTGGAGAAGTGGCTGCAACTGATGCTGATGT
GGCACCCCCGACAGAGGGGCACGGATCCCACGTATGGGCCCAATGGCTGC
TTCAAGGCCCTGGATGACATCTTAAACTTAAAGCTGGTTCATATCTTGAA
CATGGTCACGGGCACCATCCACACCTACCCTGTGACAGAGGATGAGAGTC
TGCAGAGCTTGAAGGCCAGAATCCAACAGGACACGGGCATCCCAGAGGAG
GACCAGGAGCTGCTGCAGGAAGCGGGCCTGGCGTTGATCCCCGATAAGCC
TGCCACTCAGTGTATTTCAGACGGCAAGTTAAATGAGGGCCACACATTGG
ACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCACCTATGAGACT
CAGATCTCCCCACGGCCCCAACCTGAAAGTGTCAGCTGTATCCTTCAAGA
GCCCAAGAGGAATCTCGCCTTCTTCCAGCTGAGGAAGGTGTGGGGCCAGG
TCTGGCACAGCATCCAGACCCTGAAGGAAGATTGCAACCGGCTGCAGCAG
GGACAGCGAGCCGCCATGATGAATCTCCTCCGAAACAACAGCTGCCTCTC
CAAAATGAAGAATTCCATGGCTTCCATGTCTCAGCAGCTCAAGGCCAAGT
TGGATTTCTTCAAAACCAGCATCCAGATTGACCTGGAGAAGTACAGCGAG
CAAACCGAGTTTGGGATCACATCAGATAAACTGCTGCTGGCCTGGAGGGA
AATGGAGCAGGCTGTGGAGCTCTGTGGGCGGGAGAACGAAGTGAAACTCC
TGGTAGAACGGATGATGGCTCTGCAGACCGACATTGTGGACTTACAGAGG
AGCCCCATGGGCCGGAAGCAGGGGGGAACGCTGGACGACCTAGAGGAGCA
AGCAAGGGAGCTGTACAGGAGACTAAGGGAAAAACCTCGAGACCAGCGAA
CTGAGGGTGACAGTCAGGAAATGGTACGGCTGCTGCTTCAGGCAATTCAG
AGCTTCGAGAAGAAAGTGCGAGTGATCTATACGCAGCTCAGTAAAACTGT
GGTTTGCAAGCAGAAGGCGCTGGAACTGTTGCCCAAGGTGGAAGAGGTGG
TGAGCTTAATGAATGAGGATGAGAAGACTGTTGTCCGGCTGCAGGAGAAG
CGGCAGAAGGAGCTCTGGAATCTCCTGAAGATTGCTTGTAGCAAGGTCCG
TGGTCCTGTCAGTGGAAGCCCGGATAGCATGAATGCCTCTCGACTTAGCC
AGCCTGGGCAGCTGATGTCTCAGCCCTCCACGGCCTCCAACAGCTTACCT
GAGCCAGCCAAGAAGAGTGAAGAACTGGTGGCTGAAGCACATAACCTCTG
CACCCTGCTAGAAAATGCCATACAGGACACTGTGAGGGAACAAGACCAGA
GTTTCACGCCCTAGGCGGCCGC

Amino Acid Sequence

MHHHHHHMSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAI
KQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPL
LAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIH
RDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPEL
LEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDI
VVSEDLNGTVKFSSSLPYPNNLNSVLSERLEKWLQLMLMWHPRQRGTDPT
YGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQD
TGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNS
KITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKED
CNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQID
LEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTD
IVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRL
LLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTV
VRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPST
ASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3167
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression. Protein is truncated at residue 742 in comparison to genbank and has an extra P at C terminus. Also differs at A260S. (gi 14738764 revised)