cDNA Clone CCS

• Plasmid: pEBG-2T-CCS
• Parent Plasmid: pEBG2T
• Expressed: GST-CCS
• Genbank: NP_005116
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCTTCGGATTCGGGGAACCAGGGGACCCTCTGCACGTTGGAGTT
CGCGGTGCAGATGACCTGTCAGAGCTGTGTGGACGCGGTGCGCAAATCCC
TGCAAGGGGTGGCAGGTGTCCAGGATGTGGAGGTGCACTTGGAGGACCAG
ATGGTCTTGGTACACACCACTCTACCCAGCCAGGAGGTGCAGGCTCTCCT
GGAAGGCACGGGGCGGCAGGCGGTACTCAAGGGCATGGGCAGCGGCCAGT
TGCAGAATCTGGGGGCAGCAGTGGCCATCCTGGGGGGGCCTGGCACCGTG
CAGGGGGTGGTGCGCTTCCTACAGCTGACCCCTGAGCGCTGCCTCATCGA
GGGAACTATTGACGGCCTGGAGCCTGGGCTGCATGGACTCCACGTCCATC
AGTACGGGGACCTTACAAACAACTGCAACAGCTGTGGGAATCACTTTAAC
CCTGATGGAGCATCTCATGGGGGCCCCCAGGACTCTGACCGGCACCGCGG
AGACCTGGGCAATGTCCGTGCTGATGCTGACGGCCGCGCCATCTTCAGAA
TGGAGGATGAGCAGCTGAAGGTGTGGGATGTGATTGGCCGCAGCCTGATT
ATTGATGAGGGAGAAGATGACCTGGGCCGGGGAGGCCATCCCTTATCCAA
GATCACAGGGAACTCCGGGGAGAGGTTGGCCTGTGGCATCATTGCACGCT
CCGCTGGCCTTTTCCAGAACCCCAAGCAGATCTGCTCTTGCGATGGCCTC
ACCATCTGGGAGGAGCGAGGCCGGCCCATCGCTGGCAAGGGCCGAAAGGA
GTCAGCGCAGCCCCCTGCCCACCTTTGAGGATCAAGGGCGAATTCTGCAG
ATATCCATCACACTGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSASDSGNQGTLCTLEFAVQMTCQSC
VDAVRKSLQGVAGVQDVEVHLEDQMVLVHTTLPSQEVQALLEGTGRQAVL
KGMGSGQLQNLGAAVAILGGPGTVQGVVRFLQLTPERCLIEGTIDGLEPG
LHGLHVHQYGDLTNNCNSCGNHFNPDGASHGGPQDSDRHRGDLGNVRADA
DGRAIFRMEDEQLKVWDVIGRSLIIDEGEDDLGRGGHPLSKITGNSGERL
ACGIIARSAGLFQNPKQICSCDGLTIWEERGRPIAGKGRKESAQPPAHL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3146
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalain expression.