cDNA Clone MITF

• Plasmid: pGEX6P-1-MITF 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-MITF 6His
• Genbank: I38024
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCCTGGAAATGCTAGAATATAATCACTATCAGGTGCAGACCCACCT
CGAAAACCCCACCAAGTACCACATACAGCAAGCCCAACGGCAGCAGGTAA
AGCAGTACCTTTCTACCACTTTAGCAAATAAACATGCCAACCAAGTCCTG
AGCTTGCCATGTCCAAACCAGCCTGGCGATCATGTCATGCCACCGGTGCC
GGGGAGCAGCGCACCCAACAGCCCCATGGCTATGCTTACGCTTAACTCCA
ACTGTGAAAAAGAGGGATTTTATAAGTTTGAAGAGCAAAACAGGGCAGAG
AGCGAGTGCCCAGGCATGAACACACATTCACGAGCGTCCTGTATGCAGAT
GGATGATGTAATCGATGACATCATTAGCCTAGAATCAAGTTATAATGAGG
AAATCTTGGGCTTGATGGATCCTGCTTTGCAAATGGCAAATACGTTGCCT
GTCTCGGGAAACTTGATTGATCTTTATGGAAACCAAGGTCTGCCCCCACC
AGGCCTCACCATCAGCAACTCCTGTCCAGCCAACCTTCCCAACATAAAAA
GGGAGCTCACAGAGTCTGAAGCAAGAGCACTGGCCAAAGAGAGGCAGAAA
AAGGACAATCACAACCTGATTGAACGAAGAAGAAGATTTAACATAAATGA
CCGCATTAAAGAACTAGGTACTTTGATTCCCAAGTCAAATGATCCAGACA
TGCGCTGGAACAAGGGAACCATCTTAAAAGCATCCGTGGACTATATCCGA
AAGTTGCAACGAGAACAGCAACGCGCAAAAGAACTTGAAAACCGACAGAA
GAAACTGGAGCACGCCAACCGGCATTTGTTGCTCAGAATACAGGAACTTG
AAATGCAGGCTCGAGCTCATGGACTTTCCCTTATTCCATCCACGGGTCTC
TGCTCTCCAGATTTGGTGAATCGGATCATCAAGCAAGAACCCGTTCTTGA
GAACTGCAGCCAAGACCTCCTTCAGCATCATGCAGACCTAACCTGTACAA
CAACTCTCGATCTCACGGATGGCACCATCACCTTCAACAACAACCTCGGA
ACTGGGACTGAGGCCAACCAAGCCTATAGTGTCCCCACAAAAATGGGATC
CAAACTGGAAGACATCCTGATGGACGACACCCTTTCTCCCGTCGGTGTCA
CTGATCCACTCCTTTCCTCAGTGTCCCCCGGAGCTTCCAAAACAAGCAGC
CGGAGGAGCAGTATGAGCATGGAAGAGACGGAGCACACTTGTCATCATCA
CCATCACCATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFLEMLEYNHYQVQTHLE
NPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPG
SSAPNSPMAMLTLNSNCEKEGFYKFEEQNRAESECPGMNTHSRASCMQMD
DVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGLPPPG
LTISNSCPANLPNIKRELTESEARALAKERQKKDNHNLIERRRRFNINDR
IKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKELENRQKK
LEHANRHLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLEN
CSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSK
LEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTCHHHH
HH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3131
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST and C terminal 6His for bacterial expression.