cDNA Clone Nogo b

• Plasmid: pEGFP-C1-Nogob S107E
• Parent Plasmid: pEGFPC1
• Expressed: GFP-Nogob S107E
• Genbank: CAB99249
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAAGACCT
GGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGC
CCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAGGAGGAAGAA
GAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGT
GCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAGTGCCCACCG
CCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCG
CCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGCCCCGGAGCG
GCAGCCGGAATGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCAT
CCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCTGAGGACGAC
GAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCA
GGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCGCGCCCCCCT
CCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGTTGTT
GACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGC
CAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAA
CAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGCTTTAGGATA
TACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATT
CAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGGTTCAGA
AGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTC
AGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGAAGTTTGCAGT
GTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACAC
TACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAA
CGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGT
TAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCA
AAGCTGAATGA

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSATMYPYDVPDYAEDLDQSPLVSSSDSPPRPQPAFKYQFV
REPEDEEEEEEEEEEDEDEDLEELEVLERKPAAGLSAAPVPTAPAAGAPL
MDFGNDFVPPAPRGPLPAAPPVAPERQPEWDPSPVSSTVPAPSPLSAAAV
SPSKLPEDDEPPARPPPPPPASVSPQAEPVWTPPAPAPAAPPSTPAAPKR
RGSSGSVVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALAL
LSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALG
HVNCTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALIS
LFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGLKRKAE*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU3124
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA tagged construct for mammalian expression.