cDNA Clone FOXO1A

• Plasmid: pGEX6P-1-FKHR 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-FKHR 6His
• Genbank: NM_002015
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCAGAGGCACCGGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGT
GGAGCTGGACCCGGAGTTCGAGCCCCAGAGCCGTCCGCGATCCTGTACGT
GGCCCCTGCAAAGGCCGGAGCTCCAAGCGAGCCCTGCCAAGCCCTCGGGG
GAGACGGCCGCTGACTCCATGATCCCCGAGGAGGAGGACGATGAAGACGA
CGAGGACGGCGGGGGACGGGCCGGCTCGGCCATGGCGATCGGCGGCGGCG
GCGGGAGCGGCACGCTGGGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGG
GTGCTGGCACCCGGAGGGCAAGACCCCGGGTCTGGGCCAGCCACCGCGGC
GGGCGGGCTGAGCGGGGGTACACAGGCGCTGCTGCAGCCTCAGCAACCGC
TGCCACCGCCGCAGCCGGGGGCGGCTGGGGGCTCCGGGCAGCCGAGGAAA
TGTTCGTCGCGGCGGAACGCCTGGGGAAACCTGTCCTACGCGGACCTGAT
CACCCGCGCCATCGAGAGCTCCCCGGACAAACGGCTCACTCTGTCCCAGA
TCTACGAGTGGATGGTGCGTTGCGTGCCCTACTTCAAGGATAAGGGCGAC
AGCAACAGCTCTGCCGGCTGGAAGAACTCCATCCGGCACAACCTGTCACT
GCATAGTCGATTCATGCGGGTCCAGAATGAGGGAACTGGCAAGAGCTCTT
GGTGGATCATCAACCCTGATGGGGGGAAGAGCGGAAAAGCCCCCCGGCGG
CGGGCTGTCTCCATGGACAATAGCAACAAGTATACCAAGAGCCGTGGCCG
CGCAGCCAAGAAGAAGGCAGCCCTGCAGACAGCCCCCGAATCAGCTGACG
ACAGTCCCTCCCAGCTCTCCAAGTGGCCTGGCAGCCCCACGTCACGCAGC
AGTGATGAGCTGGATGCGTGGACGGACTTCCGTTCACGCACCAATTCTAA
CGCCAGCACAGTCAGTGGCCGCCTGTCGCCCATCATGGCAAGCACAGAGT
TGGATGAAGTCCAGGACGATGATGCGCCTCTCTCGCCCATGCTCTACAGC
AGCTCAGCCAGCCTGTCACCTTCAGTAAGCAAGCCGTGCACGGTGGAACT
GCCACGGCTGACTGATATGGCAGGCACCATGAATCTGAATGATGGGCTGA
CTGAAAACCTCATGGACGACCTGCTGGATAACATCACGCTCCCGCCATCC
CAGCCATCGCCCACTGGGGGACTCATGCAGCGGAGCTCTAGCTTCCCGTA
TACCACCAAGGGCTCGGGCCTGGGCTCCCCAACCAGCTCCTTTAACAGCA
CGGTGTTCGGACCTTCATCTCTGAACTCCCTACGCCAGTCTCCCATGCAG
ACCATCCAAGAGAACAAGCCAGCTACCTTCTCTTCCATGTCACACTATGG
TAACCAGACACTCCAGGACCTGCTCACTTCGGACTCACTTAGCCACAGCG
ATGTCATGATGACACAGTCGGACCCCTTGATGTCTCAGGCCAGCACCGCT
GTGTCTGCCCAGAATTCCCGCCGGAACGTGATGCTTCGCAATGATCCGAT
GATGTCCTTTGCTGCCCAGCCTAACCAGGGAAGTTTGGTCAATCAGAACT
TGCTCCACCACCAGCACCAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCC
TTGTCGAATTCTGTCAGCAACATGGGCTTGAGTGAGTCCAGCAGCCTTGG
GTCAGCCAAACACCAGCAGCAGTCTCCTGTCAGCCAGTCTATGCAAACCC
TCTCGGACTCTCTCTCAGGCTCCTCCTTGTACTCAACTAGTGCAAACCTG
CCCGTCATGGGCCATGAGAAGTTCCCCAGCGACTTGGACCTGGACATGTT
CAATGGGAGCTTGGAATGTGACATGGAGTCCATTATCCGTAGTGAACTCA
TGGATGCTGATGGGTTGGATTTTAACTTTGATTCCCTCATCTCCACACAG
AATGTTGTTGGTTTGAACGTGGGGAACTTCACTGGTGCTAAGCAGGCCTC
ATCTCAGAGCTGGGTGCCAGGCCATCATCACCATCACCATTAAGTCGACG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAEAPQVVEIDPDFEPLPRP
RSCTWPLPRPEFSQSNSATSSPAPSGSAAANPDAAAGLPSASAAAVSADF
MSNLSLLEESEDFPQAPGSVAAAVAAAAAAAATGGLCGDFQGPEAGCLHP
APPQPPPPGPLSQHPPVPPAAAGPLAGQPRKSSSSRRNAWGNLSYADLIT
KAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLH
SKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRSRA
AKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSN
ASTISGRLSPIMTEQDDLGEGDVHSMVYPPSAAKMASTLPSLSEISNPEN
MENLLDNLNLLSSPTSLTVSTQSSPGTMMQQTPCYSFAPPNTSLNSPSPN
YQKYTYGQSSMSPLPQMPIQTLQDNKSSYGGMSQYNCAPGLLKELLTSDS
PPHNDIMTPVDPGVAQPNSRVLGQNVMMGPNSVMSTYGSQASHNKMMNPS
SHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKTPVQVPLPHPM
QMSALGGYSSVSSCNGYGRMGLLHQEKLPSDLDGMFIERLDCDMESIIRN
DLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1725
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST and C terminal 6His for bacterial expression