cDNA Clone CBS

• Plasmid: pEGFP-C2-HA-CBS
• Parent Plasmid: pEGFPC2
• Expressed: GFP-HA CBS
• Genbank: P35520
• Tag: GFP
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCCTTCTGA
GACCCCCCAGGCAGAAGTGGGGCCCACAGGCTGCCCCCACCGCTCAGGGC
CACACTCGGCGAAGGGGAGCCTGGAGAAGGGGTCCCCAGAGGATAAGGAA
GCCAAGGAGCCCCTGTGGATCCGGCCCGATGCTCCGAGCAGGTGCACCTG
GCAGCTGGGCCGGCCTGCCTCCGAGTCCCCACATCACCACACTGCCCCGG
CAAAATCTCCAAAAATCTTGCCAGATATTCTGAAGAAAATCGGGGACACC
CCTATGGTCAGAATCAACAAGATTGGGAAGAAGTTCGGCCTGAAGTGTGA
GCTCTTGGCCAAGTGTGAGTTCTTCAACGCGGGCGGGAGCGTGAAGGACC
GCATCAGCCTGCGGATGATTGAGGATGCTGAGCGCGACGGGACGCTGAAG
CCCGGGGACACGATTATCGAGCCGACATCCGGGAACACCGGGATCGGGCT
GGCCCTGGCTGCGGCAGTGAGGGGCTATCGCTGCATCATCGTGATGCCAG
AGAAGATGAGCTCCGAGAAGGTGGACGTGCTGCGGGCACTGGGGGCTGAG
ATTGTGAGGACGCCCACCAATGCCAGGTTCGACTCCCCGGAGTCACACGT
GGGGGTGGCCTGGCGGCTGAAGAACGAAATCCCCAATTCTCACATCCTAG
ACCAGTACCGCAACGCCAGCAACCCCCTGGCTCACTATGACACCACCGCT
GATGAGATCCTGCAGCAGTGTGATGGGAAGCTGGACATGCTGGTGGCTTC
AGTGGGCACGGGCGGCACCATCACGGGCATTGCCAGGAAGCTGAAGGAGA
AGTGTCCTGGATGCAGGATCATTGGGGTGGATCCCGAAGGGTCCATCCTC
GCAGAGCCGGAGGAGCTGAACCAGACGGAGCAGACAACCTACGAGGTGGA
AGGGATCGGCTACGACTTCATCCCCACGGTGCTGGACAGGACGGTGGTGG
ACAAGTGGTTCAAGAGCAACGATGAGGAGGCGTTCACCTTTGCCCGCATG
CTGATCGCGCAAGAGGGGCTGCTGTGCGGTGGCAGTGCTGGCAGCACGGT
GGCGGTGGCCGTGAAGGCCGCGCAGGAGCTGCAGGAGGGCCAGCGCTGCG
TGGTCATTCTGCCCGACTCAGTGCGGAACTACATGACCAAGTTCCTGAGC
GACAGGTGGATGCTGCAGAAGGGCTTTCTGAAGGAGGAGGACCTCACGGA
GAAGAAGCCCTGGTGGTGGCACCTCCGTGTTCAGGAGCTGGGCCTGTCAG
CCCCGCTGACCGTGCTCCCGACCATCACCTGTGGGCACACCATCGAGATC
CTCCGGGAGAAGGGCTTCGACCAGGCGCCCGTGGTGGATGAGGCGGGGGT
AATCCTGGGAATGGTGACGCTTGGGAACATGCTCTCGTCCCTGCTTGCCG
GGAAGGTGCAGCCGTCAGACCAAGTTGGCAAAGTCATCTACAAGCAGTTC
AAACAGATCCGCCTCACGGACACGCTGGGCAGGCTCTCGCACATCCTGGA
GATGGACCACTTCGCCCTGGTGGTGCACGAGCAGATCCAGTACCACAGCA
CCGGGAAGTCCAGTCAGCGGCAGATGGTGTTCGGGGTGGTCACCGCCATT
GACTTGCTGAACTTCGTGGCCGCCCAGGAGCGGGACCAGAAGTGAGAATT
C

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFATMYPYDVPDYAPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKE
AKEPLWIRPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDT
PMVRINKIGKKFGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLK
PGDTIIEPTSGNTGIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAE
IVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTA
DEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSIL
AEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARM
LIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLS
DRWMLQKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEI
LREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQF
KQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAI
DLLNFVAAQERDQK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1682
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA tag for mammalian expression