cDNA Clone CBS

• Plasmid: pCMV5-HA CBS
• Parent Plasmid: pCMV5
• Expressed: HA-CBS
• Genbank: P35520
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCCTTCTGA
GACCCCCCAGGCAGAAGTGGGGCCCACAGGCTGCCCCCACCGCTCAGGGC
CACACTCGGCGAAGGGGAGCCTGGAGAAGGGGTCCCCAGAGGATAAGGAA
GCCAAGGAGCCCCTGTGGATCCGGCCCGATGCTCCGAGCAGGTGCACCTG
GCAGCTGGGCCGGCCTGCCTCCGAGTCCCCACATCACCACACTGCCCCGG
CAAAATCTCCAAAAATCTTGCCAGATATTCTGAAGAAAATCGGGGACACC
CCTATGGTCAGAATCAACAAGATTGGGAAGAAGTTCGGCCTGAAGTGTGA
GCTCTTGGCCAAGTGTGAGTTCTTCAACGCGGGCGGGAGCGTGAAGGACC
GCATCAGCCTGCGGATGATTGAGGATGCTGAGCGCGACGGGACGCTGAAG
CCCGGGGACACGATTATCGAGCCGACATCCGGGAACACCGGGATCGGGCT
GGCCCTGGCTGCGGCAGTGAGGGGCTATCGCTGCATCATCGTGATGCCAG
AGAAGATGAGCTCCGAGAAGGTGGACGTGCTGCGGGCACTGGGGGCTGAG
ATTGTGAGGACGCCCACCAATGCCAGGTTCGACTCCCCGGAGTCACACGT
GGGGGTGGCCTGGCGGCTGAAGAACGAAATCCCCAATTCTCACATCCTAG
ACCAGTACCGCAACGCCAGCAACCCCCTGGCTCACTATGACACCACCGCT
GATGAGATCCTGCAGCAGTGTGATGGGAAGCTGGACATGCTGGTGGCTTC
AGTGGGCACGGGCGGCACCATCACGGGCATTGCCAGGAAGCTGAAGGAGA
AGTGTCCTGGATGCAGGATCATTGGGGTGGATCCCGAAGGGTCCATCCTC
GCAGAGCCGGAGGAGCTGAACCAGACGGAGCAGACAACCTACGAGGTGGA
AGGGATCGGCTACGACTTCATCCCCACGGTGCTGGACAGGACGGTGGTGG
ACAAGTGGTTCAAGAGCAACGATGAGGAGGCGTTCACCTTTGCCCGCATG
CTGATCGCGCAAGAGGGGCTGCTGTGCGGTGGCAGTGCTGGCAGCACGGT
GGCGGTGGCCGTGAAGGCCGCGCAGGAGCTGCAGGAGGGCCAGCGCTGCG
TGGTCATTCTGCCCGACTCAGTGCGGAACTACATGACCAAGTTCCTGAGC
GACAGGTGGATGCTGCAGAAGGGCTTTCTGAAGGAGGAGGACCTCACGGA
GAAGAAGCCCTGGTGGTGGCACCTCCGTGTTCAGGAGCTGGGCCTGTCAG
CCCCGCTGACCGTGCTCCCGACCATCACCTGTGGGCACACCATCGAGATC
CTCCGGGAGAAGGGCTTCGACCAGGCGCCCGTGGTGGATGAGGCGGGGGT
AATCCTGGGAATGGTGACGCTTGGGAACATGCTCTCGTCCCTGCTTGCCG
GGAAGGTGCAGCCGTCAGACCAAGTTGGCAAAGTCATCTACAAGCAGTTC
AAACAGATCCGCCTCACGGACACGCTGGGCAGGCTCTCGCACATCCTGGA
GATGGACCACTTCGCCCTGGTGGTGCACGAGCAGATCCAGTACCACAGCA
CCGGGAAGTCCAGTCAGCGGCAGATGGTGTTCGGGGTGGTCACCGCCATT
GACTTGCTGAACTTCGTGGCCGCCCAGGAGCGGGACCAGAAGTGAGAATT
C

Amino Acid Sequence

MYPYDVPDYAPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEP
LWIRPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVR
INKIGKKFGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDT
IIEPTSGNTGIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRT
PTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEIL
QQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPE
ELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQ
EGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWM
LQKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREK
GFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIR
LTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLN
FVAAQERDQK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1673
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression