cDNA Clone Filamin gamma

• Plasmid: pCMV-FLAG Filamin frag S2213D
• Parent Plasmid: pCMV5
• Expressed: FLAG-Filamin frag S2213D
• Genbank: XP_045856
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCACCATGGACTACAAGGACGACGATGACAAGAAGCACATCCC
GGGGAGCCCCTTCACAGCCAAGATCACAGGTGATGACTCCATGAGGACCT
CACAGCTGAATGTGGGCACCTCCACGGACGTGTCACTGAAGATCACCGAG
AGTGATCTGAGCCAGCTGACCGCCAGCATCCGTGCCCCCTCGGGCAACGA
GGAGCCCTGCCTGCTGAAGCGCCTGCCCAACCGGCACATTGGGATCTCCT
TCACCCCCAAGGAGGTCGGGGAGCACGTGGTGAGCGTGCGCAAGAGTGGC
AAGCATGTCACCAACAGCCCCTTCAAGATCCTGGTGGGGCCATCTGAGAT
CGGGGACGCCAGCAAGGTGCGGGTCTGGGGCAAGGGGCTTTCCGAGGGAC
ACACATTCCAGGTGGCAGAGTTCATCGTGGACACTCGCAATGCAGGTTAT
GGGGGCTTGGGGCTGAGTATTGAAGGCCCAAGCAAGGTGGACATCAACTG
TGAGGACATGGAGGACGGGACATGCAAAGTCACCTACTGCCCCACCGAGC
CCGGCACCTACATCATCAACATCAAGTTTGCTGACAAGCACGTGCCTGGA
AGCCCCTTCACTGTGAAGGTGACCGGCGAGGGCCGCATGAAGGAGAGCAT
CACCCGGCGGAGACAGGCACCTTCCATCGCCACCATCGGCAGCACCTGTG
ACCTCAACCTCAAGATCCCAGGAAACTGGTTCCAGATGGTGTCTGCCCAG
GAGCGCCTGACACGCACCTTCACACGCAGCAGCCACACCTACACCCGCAC
GGAGCGCACGGAGATCAGCAAGACGCGGGGCGGGGAGACAAAGCGCGAGG
TGCGGGTGGAGGAGTCCACCCAGGTCGGCGGGGACCCCTTCCCTGCTGTG
TTTGGGGACTTCCTGGGCCGGGAGCGCCTGGGAGACTTCGGCAGCATCAC
CCGGCAGCAGGAGGGTGAGGCCAGCTCTCAGGACATGACTGCACAGGTGA
CCAGCCCATCGGGCAAGGTGGAAGCCGCAGAGATCGTCGAGGGCGAGGAC
AGCGCCTACAGCGTGCGCTTTGTGCCCCAGGAAATGGGGCCCCATACGGT
CGCTGTCAAGTACCGTGGCCAGCACGTGCCCGGCAGCCCCTTTCAGTTCA
CTGTGGGGCCGCTGGGTGAAGGTGGTGCCCACAAGGTGCGGGCCGGAGGC
ACAGGGCTGGAGCGAGGTGTGGCCGGCGTGCCAGCCGAGTTCAGCATCTG
GACCCGGGAGGCTGGCGCTGGGGGCCTGTCCATTGCTGTGGAGGGTCCTA
GCAAAGCGGAGATTGCATTTGAGGATCGCAAAGATGGCTCCTGCGGCGTC
TCCTATGTCGTCCAGGAACCAGGTGACTATGAGGTCTCCATCAAGTTCAA
TGATGAGCACATCCCAGACAGCCCCTTTGTGGTGCCTGTGGCCTCCCTCT
CGGATGACGCTCGCCGTCTCACTGTCACCAGCCTCCAGGAGACGGGGCTC
AAGGTGAACCAGCCAGCGTCCTTTGCCGTGCAGCTGAACGGTGCCCGGGG
CGTGATTGATGCCCGGGTGCACACACCCTCGGGGGCTGTGGAGGAGTGCT
ACGTCTCTGAGCTGGACAGTGACAAGCACACCATCCGTTGAGTCGAC

Amino Acid Sequence

MDYKDDDDKKHIPGSPFTAKITGDDSMRTSQLNVGTSTDVSLKITESDLS
QLTASIRAPSGNEEPCLLKRLPNRHIGISFTPKEVGEHVVSVRKSGKHVT
NSPFKILVGPSEIGDASKVRVWGKGLSEGHTFQVAEFIVDTRNAGYGGLG
LSIEGPSKVDINCEDMEDGTCKVTYCPTEPGTYIINIKFADKHVPGSPFT
VKVTGEGRMKESITRRRQAPSIATIGSTCDLNLKIPGNWFQMVSAQERLT
RTFTRSSHTYTRTERTEISKTRGGETKREVRVEESTQVGGDPFPAVFGDF
LGRERLGDFGSITRQQEGEASSQDMTAQVTSPSGKVEAAEIVEGEDSAYS
VRFVPQEMGPHTVAVKYRGQHVPGSPFQFTVGPLGEGGAHKVRAGGTGLE
RGVAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSCGVSYVV
QEPGDYEVSIKFNDEHIPDSPFVVPVASLSDDARRLTVTSLQETGLKVNQ
PASFAVQLNGARGVIDARVHTPSGAVEECYVSELDSDKHTIR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1602
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal FLAG tagged Filamin D20