cDNA Clone GYS1

• Plasmid: pACYCDuet-1 Glycogen Synthase
• Parent Plasmid: pACYC Duet1
• Expressed: 6His-Glycogen Synthase
• Genbank: NM_002103
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACCCTTTAAACCGCACTTTGTCCATGTCCTCACTGCCAGGACTGGA
GGACTGGGAGGATGAATTCGACCTGGAGAACGCAGTGCTCTTCGAAGTGG
CCTGGGAGGTGGCTAACAAGGTGGGTGGCATCTACACGGTGCTGCAGACG
AAGGCGAAGGTGACAGGGGACGAATGGGGCGACAACTACTTCCTGGTGGG
GCCGTACACGGAGCAGGGCGTGAGGACCCAGGTGGAACTGCTGGAGGCCC
CCACCCCGGCCCTGAAGAGGACACTGGATTCCATGAACAGCAAGGGCTGC
AAGGTGTATTTCGGGCGCTGGCTGATCGAGGGAGGCCCTCTGGTGGTGCT
CCTGGACGTGGGTGCCTCAGCTTGGGCCCTGGAGCGCTGGAAGGGAGAGC
TCTGGGATACCTGCAACATCGGAGTGCCGTGGTACGACCGCGAGGCCAAC
GACGCTGTCCTCTTTGGCTTTCTGACCACCTGGTTCCTGGGTGAGTTCCT
GGCACAGAGTGAGGAGAAGCCACATGTGGTTGCTCACTTCCATGAGTGGT
TGGCAGGCGTTGGACTCTGCCTGTGTCGTGCCCGGCGACTGCCTGTAGCA
ACCATCTTCACCACCCATGCCACGCTGCTGGGGCGCTACCTGTGTGCCGG
TGCCGTGGACTTCTACAACAACCTGGAGAACTTCAACGTGGACAAGGAAG
CAGGGGAGAGGCAGATCTACCACCGATACTGCATGGAAAGGGCGGCAGCC
CACTGCGCTCACGTCTTCACTACTGTGTCCCAGATCACCGCCATCGAGGC
ACAGCACTTGCTCAAGAGGAAACCAGATATTGTGACCCCCAATGGGCTGA
ATGTGAACAAGTTTTCTGCCATGCATGAGTTCCAGAACCTCCATGCTCAG
AGCAAGGCTCGAATCCAGGAGTTTGTGCGGGGCCATTTTTATGGGCATCT
GGACTTCAACTTGGACAAGACCTTATACTTCTTTATCGCCGGCCGCTATG
AGTTCTCCAACAAGGGTGCTGACGTCTTCCTGGAGGCATTGGCTCGGCTC
AACTATCTGCTCAGAGTGAACGGCAGCGAGCAGACAGTGGTTGCCTTCTT
CATCATGCCAGCGCGGACCAACAATTTCAACGTGGAAACCCTCAAAGGCC
AAGCTGTGCGCAAACAGCTTTGGGACACGGCCAACACGGTGAAGGAAAAG
TTCGGGAGGAAGCTTTATGAATCCTTACTGGTTGGGAGCCTTCCCGACAT
GAACAAGATGCTGGATAAGGAAGACTTCACTATGATGAAGAGAGCCATCT
TTGCAACGCAGCGGCAGTCTTTCCCCCCTGTGTGCACCCACAATATGCTG
GATGACTCCTCAGACCCCATCCTGACCACCATCCGCCGAATCGGCCTCTT
CAATAGCAGTGCCGACAGGGTGAAGGTGATTTTCCACCCGGAGTTCCTCT
CCTCCACAAGCCCCCTGCTCCCTGTGGACTATGAGGAGTTTGTCCGTGGC
TGTCACCTTGGAGTCTTCCCCTCCTACTATGAGCCTTGGGGCTACACACC
GGCTGAGTGCACGGTTATGGGAATCCCCAGTATCTCCACCAATCTCTCCG
GCTTCGGCTGCTTCATGGAGGAACACATCGCAGACCCCTCAGCTTACGGT
ATCTACATTCTTGACCGGCGGTTCCGCAGCCTGGATGATTCCTGCTCGCA
GCTCACCTCCTTCCTCTACAGTTTCTGTCAGCAGAGCCGGCGGCAGCGTA
TCATCCAGCGGAACCGCACGGAGCGCCTCTCCGACCTTCTGGACTGGAAA
TACCTAGGCCGGTACTATATGTCTGCGCGCCACATGGCGCTGTCCAAGGC
CTTTCCAGAGCACTTCACCTACGAGCCCAACGAGGCGGATGCGGCCCAGG
GGTACCGCTACCCACGGCCAGCCTCGGTGCCACCGTCGCCCTCGCTGTCA
CGACACTCCAGCCCGCACCAGAGTGAGGATGAGGAGGATCCCCGGAACGG
GCCGCTGGAGGAAGACGGCGAGCGCTACGATGAGGACGAGGAGGCCGCCA
AGGACCGGCGCAACATCCGTGCACCA*AGTGGCCGCGCCGAGCGTCCTGC
ACCTCCTCCACCAGCGGCAGCAAGCGCAACTCTGTGGACACGGCCACCTC
CAGCTCACTCAGCACCCCGAGCGAGCCCCTCAGCCCCACCAGCTCCCTGG
GCGAGGAGCGTAACTAAGTCGAC

Amino Acid Sequence

MGSSHHHHHHSQDPNSSSARLQVDPLNRTLSMSSLPGLEDWEDEFDLENA
VLFEVAWEVANKVGGIYTVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQV
ELLEAPTPALKRTLDSMNSKGCKVYFGRWLIEGGPLVVLLDVGASAWALE
RWKGELWDICNIGVPWYDREANDAVLFGFLTTWFLGEFLAQSEEKPHVVA
HFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAVDFYNNLENF
NVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDIV
TPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNLDKTLYFF
IAGRYEFSNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNV
ETLKGQAVRKQLWDTANTVKEKFGRKLYESLLVGSLPDMNKMLDKEDFTM
MKRAIFATQRQSFPPVCTHNMLDDSSDPILTTIRRIGLFNSSADRVKVIF
HPEFLSSTSPLLPVDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSI
STNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDDSCSQLTSFLYSFCQQ
SRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYEPNE
ADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDE
DEEAAKDRRNIRAPEWPRRASCTSSTSGRKRNSVDTATSSSLSTPSEPLS
PTSSLGEERN*

• Vector Type: Bacterial
• Antibiotic: Chloramphenicol
• DU Number: DU1539
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee