cDNA Clone ERK2

• Synonyms: MAPK1, MAPK2
• Plasmid: pMal ERK2
• Parent Plasmid: pMALP2X
• Expressed: MBP-ERK2
• Genbank: NM_002745
• Tag: MBP
• Species: Human

Sequence of Insert

gcgtcgacgcggcggcggcggcggcgggcgcgggcccggagatggtccgc
gggcaggtgttcgacgtggggccgcgctacaccaacctctcgtacatcgg
cgagggcgcctacggcatggtgtgctctgcttatgataatgtcaacaaag
ttcgagtagctatcaagaaaatcagcccctttgagcaccagacctactgc
cagagaaccctgagggagataaaaatcttactgcgcttcagacatgagaa
catcattggaatcaatgacattattcgagcaccaaccatcgagcaaatga
aagatgtatatatagtacaggacctcatggaaacagatctttacaagctc
ttgaagacacaacacctcagcaatgaccatatctgctattttctctacca
gatcctcagagggttaaaatatatccattcagctaacgttctgcaccgtg
acctcaagccttccaacctgctgctcaacaccacctgtgatctcaagatc
tgtgactttggcctggcccgtgttgcagatccagaccatgatcacacagg
gttcctgacagaatatgtggccacacgttggtacagggctccagaaatta
tgttgaattccaagggctacaccaagtccattgatatttggtctgtaggc
tgcattctggcagaaatgctttctaacaggcccatctttccagggaagca
ttatcttgaccagctgaaacacattttgggtattcttggatccccatcac
aagaagacctgaattgtataataaatttaaaagctaggaactatttgctt
tctcttccacacaaaaataaggtgccatggaacaggctgttcccaaatgc
tgactccaaagctctggacttattggacaaaatgttgacattcaacccac
acaagaggattgaagtagaacaggctctggcccacccatatctggagcag
tattacgacccgagtgacgagcccatcgccgaagcaccattcaagttcga
catggaattggatgacttgcctaaggaaaagctcaaagaactaatttttg
aagagactgctagattccagccaggatacagatcttaagcggccgcgc

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFGSSRVDAAAAAAGAGPEMVRGQVFDVGPRYTNLS
YIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFR
HENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYF
LYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHD
HTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFP
GKHYLDQLKHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLF
PNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPF
KFDMELDDLPKEKLKELIFEETARFQPGYRS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU964
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal MBP tag for bacterial expression