cDNA Clone c-NAP1

• Synonyms: CEP250
• Plasmid: pMBP c-NAP1 delta N-1850
• Parent Plasmid: pMALc2X
• Expressed: pMBP c-NAP1 delta N-1850
• Genbank: AF049105
• Tag: MBP
• Species: Human

Sequence of Insert

CATATGACACTGAAGGAGCGTCATGGACAGCTTCATGACCACAAGGAACA
GGCACGAAGGCTGGAGGAAGAGCTGGCAGTGGAGGGACGGCGGGTCCAGG
CCCTGGAGGAGGTGCTGGGAGACCTAAGGGCTGAGTCTCGGGAACAGGAG
AAAGCTCTGTTGGCCCTCCAGCAGCAGTGTGCTGAGCAGGCACAGGAGCA
TGAGGTGGAGACCAGGGCCCTGCAGGACAGCTGGCTGCAGGCCCAGGCAG
TGCTCAAGGAACGGGACCAGGAGCTGGAAGCTCTGCGGGCAGAAAGTCAG
TCCTCCCGGCATCAGGAGGAGGCTGCCCGGGCCCGGGCTGAGGCTCTGCA
GGAGGCCCTTGGCAAGGCTCATGCTGCCCTGCAGGGGAAAGAGCAGCATC
TCCTCGAGCAGGCAGAATTGAGCCGCAGTCTGGAGGCCACCACTGAAACC
CTGCAAGCCTCCCTGGATGCCTGCCAGGCACACAGTCGGCAGCTGGAGGA
GGCTCTGAGGATACAAGAAGGTGAGATCCAGGACCAGGATCTCCGATACC
AGGAGGATGTGCAGCAGCTGCAGCAGGCACTTGCCCAGAGGGATGAAGAG
CTGAGACATCAGCAGGAACGGGAGCAGCTGCTGGAGAAGTCTCTGGCCCA
GAGGGTCCAAGAGAATATGATCCAAGAGAAGCAGAATCTGGGGCAAGAGA
GAGAAGAGGAGGAGATAAGGGGCCTTCATCAGAGTGTAAGGGAGCTACAG
CTGACTCTAGCCCAAAAGGAACAGGAGATTCTGGAGCTGAGGGAGACCCA
GCAAAGGAACAACCTGGAAGCCTTACCCCACAGCCACAAAACCTCCCCAA
TGGAGGAACAATCTCTAAAACTTGATTCTTTAGAGCCCAGGCTGCAGCGG
GAGCTGGAGCGGCTACAGGCAGCCCTGAGACAGACAGAAGCCAGGGAGAT
TGAGTGGAGGGAGAAGGCCCAGGACTTGGCACTCTCCCTAGCGCAGACCA
AGGCCAGTGTCAGCAGTCTGCAGGAGGTAGCCATGTTCCTACAAGCCTCT
GTCCTGGAGCGGGACTCAGAACAGCAAAGGCTGCAGGATGAACTGGAGCT
CACCAGACGGGCTCTGGAGAAGGAGCGGCTACACAGCCCAGGTGCAACCA
GCACAGCAGAACTGGGGTCCAGAGGGGAGCAGGGTGTGCAGCTGGGAGAG
GTCTCAGGAGTGGAGGCTGAGCCTAGTCCTGATGGAATGGAGAAGCAGTC
ATGGAGACAAAGGCTTGAACACCTGCAGCAAGCAGTGGCCCGGCTGGAGA
TTGACAGGAGCAGGCTGCAGCGCCACAATGTCCAGCTGCGGAGTACCTTG
GAGCAGGTGGAGCGAGAACGGAGGAAGCTGAAGAGGGAGGCCATGCGTGC
GGCCCAGGCAGGGTCCCTAGAGATCAGCAAGGCCACGGCTTCTTCACCCA
CACAGCAGGATGGGAGAGGACAGAAGAACTCAGATGCCAAGTGTGTGGCT
GAACTGCAGAAAGAGGTGGTCCTGCTGCAAGCTCAGCTGACTTTGGAGCG
GAAGCAGAAGCAGGACTACATCACCCGCTCAGCACAGACCAGCCGTGAGC
TAGCAGGCCTGCACCACAGCCTCTCACACTCACTTCTTGCCGTGGCCCAG
GCCCCTGAGGCCACTGTCCTGGAGGCAGAGACCCGCAGGCTGGATGAGTC
CCTGACTCAAAGTCTGACATCCCCAGGGCCAGTCCTGCTACACCCCAGCC
CCAGCACTACCCAAGCCGCCTCCAGGTAG

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFMAYPYDVPDYASLGGHMTLKERHGQLHDHKEQAR
RLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEV
ETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEA
LGKAHAALQGKEQHLLEQAELSRSLEATTETLQASLDACQAHSRQLEEAL
RIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRV
QENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQR
NNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEW
REKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTR
RALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWR
QRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQ
AGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQ
KQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLT
QSLTSPGPVLLHPSPSTTQAASR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU788
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct with N terminal MBP and HA tags; parental is pMALc2X with HA tag at EcoRI site. C-NAP1 insert has additional 3' UTR sequence.