cDNA Clone CLK2

• Plasmid: pFBHTb CLK2
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-CLK2
• Genbank: NM_003993
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccccgcatcctcgaaggtaccactcctcagagcgaggcagccgggg
gagttaccgtgaacactatcggagccgaaagcataagcgacgaagaagtc
gctcctggtcaagtagtagtgaccggacacgacggcgtcggcgagaggac
agctaccatgtccgttctcgaagcagttatgatgatcgttcgtccgaccg
gagggtgtatgaccggcgatactgtggcagctacagacgcaacgattata
gccgggatcggggagatgcctactatgacacagactatcggcattcctat
gaatatcagcgggagaacagcagttaccgcagccagcgcagcagccggag
gaagcacagacggcggaggaggcgcagccggacatttagccgctcatctt
cgcacagcagccggagagccaagagtgtagaggacgacgctgagggccac
ctcatctaccacgtcggggactggctacaagagcgatatgaaatcgttag
caccttaggagaggggaccttcggccgagttgtacaatgtgttgaccatc
gcaggggtggggctcgagttgccctgaagatcattaagaatgtggagaag
tacaaggaagcagctcgacttgagatcaacgtgctagagaaaatcaatga
gaaagaccctgacaacaagaacctctgtgtccagatgtttgactggtttg
actaccatggccacatgtgtatctcctttgagcttctgggccttagcacc
ttcgatttcctcaaagacaacaactacctgccctaccccatccaccaagt
gcgccacatggccttccagctgtgccaggctgtcaagttcctccatgata
acaagctgacacatacagacctcaagcctgaaaatattctgtttgtgaat
tcagactatgagctcacctacaacctagagaagaagcgagatgagcgcag
tgtgaagagcacagctgtgcgggtggtagactttggcagtgccacctttg
accatgagcaccatagcaccattgtctccactcgccattaccgagcacca
gaagtcatccttgagttgggctggtcacagccttgtgatgtgtggagtat
aggctgcatcatctttgaatactatgtgggattcaccctcttccagaccc
atgacaacagagagcatctagccatgatggaaaggatcttgggtcctatc
ccttcccggatgatccgaaagacaagaaagcagaaatatttttaccgggg
tcgcctggattgggatgagaacacatcagctgggcgctatgttcgtgaga
actgcaaaccgctgcggcggtatctgacctcagaggcagaggaacaccac
cagctcttcgatctgattgaaagcatgctagagtatgaaccagctaagcg
gctgaccttgggtgaagcccttcagcatcctttcttcgcccgccttcggg
ctgagccgcccaacaagttgtgggactccagtcgggatatcagtcggtga
gtcgac

Amino Acid Sequence

HHHHHHDYDIPTTENLYFQGAMGSPHPRRYHSSERGSRGSYREHYRSRKH
KRRRSRSWSSSSDRTRRRRREDSYHVRSRSSYDDRSSDRRVYDRRYCGSY
RRNDYSRDRGDAYYDTDYRHSYEYQRENSSYRSQRSSRRKHRRRRRRSRT
FSRSSSHSSRRAKSVEDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVV
QCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQ
MFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAV
KFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDF
GSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGF
TLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKYFYRGRLDWDENTSAG
RYVRENCKPLRRYLTSEAEEHHQLFDLIESMLEYEPAKRLTLGEALQHPF
FARLRAEPPNKLWDSSRDISR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU716
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag