cDNA Clone LCK (mouse)

• Plasmid: pFB LCK
• Parent Plasmid: pSC-A
• Expressed: 6His-LCK
• Genbank: I48845
• Tag: 6His
• Species: Mouse

Sequence of Insert

GAATTCGGCTGTGGCTGCAGCTCACACCCTGAAGATGACTGGATGGAGAA
CATTGACGTGTGTGAAAACTGCCACTATCCCATAGTCCCACTGGACAGCA
AGATCTCGCTGCCCATCCGGAATGGCTCTGAAGTGCGGGACCCACTGGTC
ACCTATGAGGGATCTCTCCCACCAGCATCCCCGCTGCAAGACAACCTGGT
TATCGCCCTGCACAGTTATGAGCCCTCCCATGATGGAGACTTGGGCTTTG
AGAAGGGTGAACAGCTCCGAATCCTGGAGCAGAGCGGTGAGTGGTGGAAG
GCTCAGTCCCTGACGACTGGCCAAGAAGGCTTCATTCCCTTCAACTTCGT
GGCGAAAGCAAACAGCCTGGAGCCTGAACCTTGGTTCTTCAAGAATCTGA
GCCGTAAGGACGCCGAGCGGCAGCTTTTGGCGCCCGGGAACACGCATGGA
TCCTTCCTGATCCGGGAAAGCGAAAGCACTGCGGGGTCCTTTTCCCTGTC
GGTCAGAGACTTCGACCAGAACCAGGGAGAAGTGGTGAAACATTACAAGA
TCCGTAACCTAGACAACGGTGGCTTCTACATCTCCCCTCGTATCACTTTT
CCCGGATTGCACGATCTAGTCCGCCATTACACCAACGCCTCTGATGGGCT
GTGCACAAAGTTGAGCCGTCCTTGCCAGACCCAGAAGCCCCAGAAACCAT
GGTGGGAGGACGAATGGGAAGTTCCCAGGGAAACACTGAAGTTGGTGGAG
CGGCTGGGAGCTGGCCAGTTCGGGGAAGTGTGGATGGGGTACTACAACGG
ACACACGAAGGTGGCGGTGAAGAGTCTGAAACAAGGGAGCATGTCCCCCG
ACGCCTTCCTGGCTGAGGCTAACCTCATGAAGCAGCTGCAGCACCCGCGG
CTAGTCCGGCTTTATGCAGTGGTCACCCAGGAACCCATCTACATCATCAC
GGAATACATGGAGAACGGGAGCCTAGTAGATTTTCTCAAGACTCCCTCGG
GCATCAAGTTGAATGTCAACAAACTTTTGGACATGGCAGCCCAGATTGCA
GAGGGCATGGCGTTCATCGAAGAACAGAATTACATCCATCGGGACCTGCG
CGCCGCCAACATCCTGGTGTCTGACACGCTGAGCTGCAAGATTGCAGACT
TTGGCCTGGCGCGCCTCATTGAGGACAATGAGTACACGGCCCGGGAGGGG
GCCAAATTTCCCATTAAGTGGACAGCACCAGAAGCCATTAACTATGGGAC
CTTCACCATCAAGTCAGACGTGTGGTCCTTCGGGATCTTGCTTACAGAGA
TCGTCACCCACGGTCGAATCCCTTACCCAGGAATGACCAACCCTGAAGTC
ATTCAGAACCTGGAGAGAGGCTACCGCATGGTGAGACCTGACAACTGTCC
GGAAGAGCTGTACCACCTCATGATGCTGTGCTGGAAGGAGCGCCCAGAGG
ACCGGCCCACGTTTGACTACCTTCGGAGTGTTCTGGATGACTTCTTCACA
GCCACAGAGGGCCAGTACCAGCCCCAGCCTGTCGACCTCTAGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFGCGCSSHPEDDWMENIDVCE
NCHYPIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPASPLQDNLVIALHS
YEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANS
LEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFD
QNQGEVVKHYKIRNLDNGGFYISPRITFPGLHDLVRHYTNASDGLCTKLS
RPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVA
VKSLKQGSMSPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMEN
GSLVDFLKTPSGIKLNVNKLLDMAAQIAEGMAFIEEQNYIHRDLRAANIL
VSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKS
DVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYH
LMMLCWKERPEDRPTFDYLRSVLDDFFTATEGQYQPQPVDL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU567
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express 6His N terminal fusion