cDNA Clone LKB1 (mouse)

• Plasmid: pGEX-LKB1 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-LKB1 6His
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGACGTGGCGGACCCCGAGCCGTTGGGCCTTTTCTCCGAGGGCGA
GCTGATGTCGGTGGGCATGGACACCTTCATCCACCGCATCGACTCCACCG
AGGTAATCTACCAGCCGCGCCGCAAACGCGCCAAGCTCATCGGCAAGTAC
CTGATGGGGGACCTGCTCGGGGAGGGCTCGTACGGCAAGGTGAAGGAGGT
GCTGGACTCCGAGACCTTATGCCGCAGGGCGGTCAAGATCCTCAAGAAGA
AAAAGCTGCGCAGGATCCCCAATGGAGAGGCCAACGTCAAGAAGGAGATC
CAGCTGCTGCGGCGGCTGCGGCATCGGAATGTGATCCAGCTTGTGGACGT
GCTGTACAATGAGGAGAAGCAGAAGATGTATATGGTGATGGAGTACTGCG
TATGTGGCATGCAGGAGATGCTGGACAGTGTGCCGGAGAAGCGCTTCCCT
GTGTGCCAAGCTCATGGGTACTTCCGCCAGCTGATTGACGGCCTGGAATA
CCTACACAGCCAGGGCATTGTTCACAAGGACATCAAGCCGGGCAACCTGC
TACTCACCACCAATGGCACACTCAAGATCTCCGACCTCGGTGTTGCCGAG
GCCCTGCACCCTTTCGCTGTGGATGACACCTGCCGGACAAGCCAGGGCTC
CCCGGCCTTCCAGCCTCCTGAGATTGCCAATGGACTGGACACCTTTTCAG
GTTTCAAGGTGGACATCTGGTCAGCTGGGGTCACACTTTACAACATCACC
ACGGGCCTGTACCCATTTGAGGGGGACAATATCTACAAGCTCTTTGAGAA
CATTGGGAGAGGAGACTTCACCATCCCTTGTGACTGCGGCCCACCACTCT
CTGACCTACTCCGAGGGATGTTGGAGTATGAGCCGGCCAAGAGGTTCTCC
ATCCGACAGATTAGGCAGCACAGCTGGTTCCGGAAGAAACACCCTCTGGC
TGAGGCGCTCGTACCTATCCCACCAAGCCCAGACACTAAGGACCGCTGGC
GCAGTATGACTGTAGTGCCCTACCTGGAGGACCTGCATGGCCGTGCGGAG
GAGGAGGAGGAGGAAGACTTGTTTGACATTGAGGACGGCATTATCTACAC
CCAGGACTTCACAGTGCCTGGACAGGTCCTGGAAGAGGAAGTGGGTCAGA
ATGGACAGAGCCACAGTTTGCCCAAGGCTGTTTGTGTGAATGGCACAGAG
CCCCAGCTCAGCAGCAAGGTGAAGCCAGAAGGCCGACCTGGCACCGCCAA
CCCTGCGCGCAAGGTGTGCTCCAGCAACAAGATCCGCCGGCTCTCGGCCT
GCAAGCAGCAGCACCATCACCATCACCATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFDVADPEPLGLFSEGEL
MSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVL
DSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHRNVIQLVDVL
YNEEKQKMYMVMEYCVCGMQEMLDSVPEKRFPVCQAHGYFRQLIDGLEYL
HSQGIVHKDIKPGNLLLTTNGTLKISDLGVAEALHPFAVDDTCRTSQGSP
AFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENI
GRGDFTIPCDCGPPLSDLLRGMLEYEPAKRFSIRQIRQHSWFRKKHPLAE
ALVPIPPSPDTKDRWRSMTVVPYLEDLHGRAEEEEEEDLFDIEDGIIYTQ
DFTVPGQVLEEEVGQNGQSHSLPKAVCVNGTEPQLSSKVKPEGRPGTANP
ARKVCSSNKIRRLSACKQQHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU557
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion, 6His C terminal fusion