cDNA Clone ARAF

• Synonyms: PKS2; A-RAF; ARAF1; RAFA1
• Plasmid: pFastbac Htb ARAF
• Parent Plasmid: pFastBac HTb
• Expressed: 6His TEV ARAF
• Genbank: BC002466
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGC
AGTGGGCACCGTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGA
CTGTCCGGGATGGCATGAGTGTCTACGACTCTCTAGACAAGGCCCTGAAG
GTGCGGGGTCTAAATCAGGACTGCTGTGTGGTCTACCGACTCATCAAGGG
ACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCCCTGGATGGCG
AGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAA
GTTTCTGTTCCATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACC
AGCATTGTTCCTCCAAGGTCCCCACAGTCTGTGTTGACATGAGTACCAAC
CGCCAACAGTTCTACCACAGTGTCCAGGATTTGTCCGGAGGCTCCAGACA
GCATGAGGCTCCCTCGAACCGCCCCCTGAATGAGTTGCTAACCCCCCAGG
GTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTCCCCTTCCCT
GCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAA
CGTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGC
TCACTGGCCAGAGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGT
GATGGAACCCCCCGGGGGAGCCCCAGCCCAGCCAGCGTGTCCTCGGGGAG
GAAGTCCCCACATTCCAAGTCACCAGCAGAGCAGCGCGAGCGGAAGTCCT
TGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGACTCAGGC
TATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGG
GACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGG
CCGTGAAGGTGCTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCT
TTCAAGAATGAGATGCAGGTGCTCAGGAAGACGCGACATGTCAACATCTT
GCTGTTTATGGGCTTCATGACCCGGCCGGGATTTGCCATCATCACACAGT
GGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCCGACACACGC
TTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCAT
GGACTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACA
ACATCTTCCTACATGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTG
GCCACAGTGAAGACTCGATGGAGCGGGGCCCAGCCCTTGGAGCAGCCCTC
AGGATCTGTGCTGTGGATGGCAGCTGAGGTGATCCGTATGCAGGACCCGA
ACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTTGTGCTCTAC
GAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCA
GATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAA
TCTCCAGCAACTGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTC
AAGTTCCAGCGGGAGGAGCGGCCCCTCTTCCCCCAGATCCTGGCCACAAT
TGAGCTGCTGCAACGGTCACTCCCCAAGATTGAGCGGAGTGCCTCGGAAC
CCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCTACTCAGC
GCAGCCCGCCTTGTGCCTTAG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFEPPRGPPANGAEPSRAVGTV
KVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTV
TAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFH
GFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAP
SNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMV
STTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPH
SKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSF
GTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMG
FMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLH
AKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVL
WMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFM
VGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQ
RSLPKIERSASEPSLHRTQADELPACLLSAARLVP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastbac Htb ARAF165.15 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU444
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression construct with N terminal 6His tag
• Low Temp: No