cDNA Clone JNK2

• Synonyms: MAPK9, SAPK1a
• Plasmid: pETBlue JNK2 6His
• Parent Plasmid: pETBlue2 original
• Expressed: JNK2 6His
• Genbank: L31951
• Tag: 6His

Sequence of Insert

GGATCCCCACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCCA
TGAGCGACAGTAAATGTGACAGTCAGTTTTATAGTGTGCAAGTGGCAGAC
TCAACCTTCACTGTCCTAAAACGTTACCAGCAGCTGAAACCAATTGGCTC
TGGGGCCCAAGGGATTGTTTGTGCTGCATTTGATACAGTTCTTGGGATAA
GTGTTGCAGTCAAGAAACTAAGCCGTCCTTTTCAGAACCAAACTCATGCA
AAGAGAGCTTATCGTGAACTTGTCCTCTTAAAATGTGTCAATCATAAAAA
TATAATTAGTTTGTTAAATGTGTTTACACCACAAAAAACTCTAGAAGAAT
TTCAAGATGTGTATTTGGTTATGGAATTAATGGATGCTAACTTATGTCAG
GTTATTCACATGGAGCTGGATCATGAAAGAATGTCCTACCTTCTTTACCA
GATGCTTTGTGGTATTAAACATCTGCATTCAGCTGGTATAATTCATAGAG
ATTTGAAGCCTAGCAACATTGTTGTGAAATCAGACTGCACCCTGAAGATC
CTTGACTTTGGCCTGGCCCGGACAGCGTGCACTAACTTCATGATGACCCC
TTACGTGGTGACACGGTACTACCGGGCGCCCGAAGTCATCCTGGGTATGG
GCTACAAAGAGAACGTTGATATCTGGTCAGTGGGTTGCATCATGGGAGAG
CTGGTGAAAGGTTGTGTGATATTCCAAGGCACTGACCATATTGATCAGTG
GAATAAAGTTATTGAGCAGCTGGGAACACCATCAGCAGAGTTCATGAAGA
AACTTCAGCCAACTGTGAGGAATTATGTCGAAAACAGACCAAAGTATCCT
GGAATCAAATTTGAAGAACTCTTTCCAGATTGGATATTCCCATCAGAATC
TGAGCGAGACAAAATAAAAACAAGTCAAGCCAGAGATCTGTTATCAAAAA
TGTTAGTGATTGATCCTGACAAGCGGATCTCTGTAGACGAAGCTCTGCGT
CACCCATACATCACTGTTTGGTATGACCCCGCCGAAGCAGAAGCCCCACC
ACCTCAAATTTATGATGCCCAGTTGGAAGAAAGAGAACATGCAATTGAAG
AATGGAAAGAGCTAATTTACAAAGAAGTCATGGATTGGGAAGAAAGAAGC
AAGAATGGTGTTGTAAAAGATCAGCCTTCAGATGCAGCAGTAAGTAGCAA
CGCCACTCCTTCTCAGTCTTCATCGATCAATGACATTTCATCCATGTCCA
CTGAGCAGACGCTGGCCTCAGACACAGACAGCAGTCTTGATGCCTCGACG
GGACCCCTTGAAGGCTGTCGATGAGCGGCCGC

Amino Acid Sequence

HHHHHHLEVLFQGPMSDSKCDSQFYSVQVADSTFTVLKRYQQLKPIGSGA
QGIVCAAFDTVLGISVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNII
SLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQML
CGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYV
VTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNK
VIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESER
DKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQ
IYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNAT
PSQSSSINDISSMSTEQTLASDTDSSLDASTGPLEGCR*

• Vector Type: Bacterial
• Antibiotic: Amp; Kan
• DU Number: DU434
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct with N terminal 6His and precission protease site