cDNA Clone Cofilin2

• Plasmid: pGEX-Cofilin2
• Parent Plasmid: pGEX6P1
• Expressed: GST-Cofilin2
• Genbank: XM_045819
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTTCTGGAGTTACAGTGAATGATGAAGTCATCAAAGTTTT
TAATGATATGAAAGTAAGGAAATCTTCTACACAAGAGGAGATCAAAAAGA
GAAAGAAAGCAGTTCTCTTCTGTTTAAGCGATGACAAAAGACAAATAATT
GTAGAGGAAGCAAAGCAGATCTTGGTGGGTGACATTGGTGATACTGTAGA
GGACCCCTACACATCTTTTGTGAAGTTGCTACCTCTGAATGATTGCCGAT
ATGCTTTGTACGATGCCACATACGAAACAAAAGAGTCTAAGAAAGAAGAC
CTAGTATTTATATTCTGGGCTCCTGAAAGTGCACCTTTAAAAAGCAAGAT
GATTTATGCTAGCTCTAAAGATGCCATTAAAAAGAAATTTACAGGTATTA
AACATGAGTGGCAAGTAAATGGCTTGGATGATATTAAGGACCGTTCGACA
CTTGGAGAGAAATTGGGAGGCAATGTAGTAGTTTCACTTGAAGGAAAACC
ATTATAAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASGVTVNDEVIKVFNDMK
VRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYT
SFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYAS
SKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU427
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression