cDNA Clone NEK2

• Plasmid: pFB Nek2A
• Parent Plasmid: pFastbac1 mod
• Expressed: 6His Nek2A
• Genbank: Z29066
• Tag: 6His
• Species: Human

Sequence of Insert

CATATGCCTTCCCGGGCTGAGGACTATGAAGTGTTGTACACCATTGGCAC
AGGCTCCTACGGCCGCTGCCAGAAGATCCGGAGGAAGAGTGATGGCAAGA
TATTAGTTTGGAAAGAACTTGACTATGGCTCCATGACAGAAGCTGAGAAA
CAGATGCTTGTTTCTGAAGTGAATTTGCTTCGTGAACTGAAACATCCAAA
CATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACAACACTGT
ACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACA
AAGGGAACCAAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGT
GATGACTCAGTTGACTCTGGCCCTGAAGGAATGCCACAGACGAAGTGATG
GTGGTCATACCGTATTGCATCGGGATCTGAAACCAGCCAATGTTTTCCTG
GATGGCAAGCAAAACGTCAAGCTTGGAGACTTTGGGCTAGCTAGAATATT
AAACCACGACACGAGTTTTGCAAAAACATTTGTTGGCACACCTTATTACA
TGTCTCCTGAACAAATGAATCGCATGTCCTACAATGAGAAATCAGATATC
TGGTCATTGGGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATT
TACAGCTTTTAGCCAGAAAGAACTCGCTGGGAAAATCAGAGAAGGCAAAT
TCAGGCGAATTCCATACCGTTACTCTGATGAATTGAATGAAATTATTACG
AGGATGTTAAACTTAAAGGATTACCATCGACCTTCTGTTGAAGAAATTCT
TGAGAACCCTTTAATAGCAGATTTGGTTGCAGACGAGCAAAGAAGAAATC
TTGAGAGAAGAGGGCGACAATTAGGAGAGCCAGAAAAATCGCAGGATTCC
AGCCCTGTATTGAGTGAGCTGAAACTGAAGGAAATTCAGTTACAGGAGCG
AGAGCGAGCTCTCAAAGCAAGAGAAGAAAGATTGGAGCAGAAAGAACAGG
AGCTTTGTGTTCGTGAGAGACTAGCAGAGGACAAACTGGCTAGAGCAGAA
AATCTGTTGAAGAACTACAGCTTGCTAAAGGAACGGAAGTTCCTGTCTCT
GGCAAGTAATCCAGAACTTCTTAATCTTCCATCCTCAGTAATTAAGAAGA
AAGTTCATTTCAGTGGGGAAAGTAAAGAGAACATCATGAGGAGTGAGAAT
TCTGAGAGTCAGCTCACATCTAAGTCCAAGTGCAAGGACCTGAAGAAAAG
GCTTCACGCTGCCCAGCTGCGGGCTCAAGCCCTGTCAGATATTGAGAAAA
ATTACCAACTGAAAAGCAGACAGATCCTGGGCATGCGCTAGCCAGGTAGA
GAGACACAGAGCTGTGTACAGGATGTAATATTACCAACCTTTAAAGACTG
ATATTCAAATGCTGTAGTGTTGAATACTTGGTTCCATGAGCCATGCCTTT
CTGTATAGTACACATGATATTTCGGAATTGGTTTTACTGTTCTTCAGCAA
CTATTGTACAAAATGTTCACATTTAATTTTTCTTTCTTCTTTTAAGAACA
TATTATAAAAAGAATACTTTCTTGGTTGGGCTTTTAATCCTGTGTGTGAT
TACTAGTAGGAACATGAGATGTGACATTCTAAATCTTGGGAGAAAAAATA
ATGTTAGGAAAAAAATATTTATGCAGGAAGAGTAGCACTCACTGAATAGT
TTTAAATGACTGAGTGGTATGCTTACAATTGTCATGTCTAGATTTAAATT
TTAAGTCTGAGATTTTAAATGTTTTTGAGCTTAGAAAACCCAGTTAGATG
CAATTTGGTCATTAATACCATGACATCTTGCTTATAAATATTCCATTGCT
CTGTAGTTCAAATCTGTTAGCTTTGTGAAAATTCATCACTGTGATGTTTG
TATTCTTTTTTTTTTTTCTGTTTAACAGAATATGAGCTGTCTGTCATTTA
CTACTTCTTTCCCACTAAATAAAGGCCTTCGTGGCCTCGAG

Amino Acid Sequence

MHHHHHHMPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGS
MTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGD
LASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLK
PANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSY
NEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDE
LNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEP
EKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAED
KLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKEN
IMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILG
MR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU406
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for baculovirus expression. Insert contains 3’UTR region.