cDNA Clone GFP-TNF alpha mRNA construct

• Plasmid: pCMV TNF alpha GFP
• Parent Plasmid: pCMV5
• Genbank: X02611
• Tag: GFP
• Species: Mouse

Sequence of Insert

AAGCTTCCTCAGCGAGGACAGCAAGGGACTAGCCAGGAGGGAGAACAGAA
ACTCCAGAACATCCTGGAAATAGCTCCCAGAAAAGCAAGCAGCCAACCAG
GCAGGTTCTGTCCCTTTCACTCACTGGCCCAAGGCGCCACATCTCCCTCC
AGAAAAGACACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGT
GCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCG
TGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAG
TTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGAC
CACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGA
AGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG
CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGT
GAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCG
ACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTAC
AACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAA
GGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCG
CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG
CCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA
TCACTCTCGGCATGGACGAGCTGTACAAGATGAGCACAGAAAGCATGATC
CGCGACGTGGAACTGGCAGAAGAGGCACTCCCCCAAAAGATGGGGGGCTT
CCAGAACTCCAGGCGGTGCCTATGTCTCAGCCTCTTCTCATTCCTGCTTG
TGGCAGGGGCCACCACGCTCTTCTGTCTACTGAACTTCGGGGTGATCGGT
CCCCAAAGGGATGAGAAGTTCCCAAATGGCCTCCCTCTCATCAGTTCTAT
GGCCCAGACCCTCACACTCAGATCATCTTCTCAAAATTCGAGTGACAAGC
CTGTAGCCCACGTCGTAGCAAACCACCAAGTGGAGGAGCAGCTGGAGTGG
CTGAGCCAGCGCGCCAACGCCCTCCTGGCCAACGGCATGGATCTCAAAGA
CAACCAACTAGTGGTGCCAGCCGATGGGTTGTACCTTGTCTACTCCCAGG
TTCTCTTCAAGGGACAAGGCTGCCCCGACTACGTGCTCCTCACCCACACC
GTCAGCCGATTTGCTATCTCATACCAGGAGAAAGTCAACCTCCTCTCTGC
CGTCAAGAGCCCCTGCCCCAAGGACACCCCTGAGGGGGCTGAGCTCAAAC
CCTGGTATGAGCCCATATACCTGGGAGGAGTCTTCCAGCTGGAGAAGGGG
GACCAACTCAGCGCTGAGGTCAATCTGCCCAAGTACTTAGACTTTGCGGA
GTCCGGGCAGGTCTACTTTGGAGTCATTGCTCTGTGAAGGGAATGGGTGT
TCATCCATTCTCTACCCAGCCCCCACTCTGACCCCTTTACTCTGACCCCT
TTATTGTCTACTCCTCAGAGCCCCCAGTCTGTGTCCTTCTAACTTAGAAA
GGGGATTATGGCTCAGAGTCCAACTCTGTGCTCAGAGCTTTCAACAACTA
CTCAGAAACACAAGATGCTGGGACAGTGACCTGGACTGTGGGCCTCTCAT
GCACCACCATCAAGGACTCAAATGGGCTTTCCGAATTCACTGGAGCCTCG
AATGTCCATTCCTGAGTTCTGCAAAGGGAGAGTGGTCAGGTTGCCTCTGT
CTCAGAATGAGGCTGGATAAGATCTCAGGCCTTCCTACCTTCAGACCTTT
CCAGACTCTTCCCTGAGGTGCAATGCACAGCCTTCCTCACAGAGCCAGCC
CCCCTCTATTTATATTTGCACTTATTATTTATTATTTATTTATTATTTAT
TTATTTGCTTATGAATGTATTTATTTGGAAGGCCGGGGTGTCCTGGAGGA
CCCAGTGTGGGAAGCTGTCTTCAGACAGACATGTTTTCTGTGAAAACGGA
GCTGAGCTGTCCCCACCTGGCCTCTCTACCTTGTTGCCTCCTCTTTTGCT
TATGTTTAAAACAAAATATTTATCTAACCCAATTGTCTTAATAACGCTGA
TTTGGTGACCAGGCTGTCGCTACATCACTGAACCTCTGCTCCCCACGGGA
GCCGTGACTGTAATTGCCCTACAGTCAATTGAGAGAAATAAAGATCGCTT
AAAATAAAAAACCCCCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKMSTESMIRDVE
LAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRD
EKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQR
ANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRF
AISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLS
AEVNLPKYLDFAESGQVYFGVIAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU245
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian construct to express 5'UTR Tnfalpha-GFP-Tnfalpha-3'UTR Tnfalpha - to be used as a reporter