cDNA Clone Nogo b

• Plasmid: pCMV Nogo b
• Parent Plasmid: pCMV5
• Expressed: HA-nogob
• Genbank: AJ251384
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAAGACCT
GGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGC
CCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAGGAGGAAGAA
GAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGT
GCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAGTGCCCACCG
CCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCG
CCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGCCCCGGAGCG
GCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCAT
CCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCTGAGGACGAC
GAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCA
GGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCGCGCCCCCCT
CCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGTTGTT
GACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGC
CAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAA
CAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGCTTTAGGATA
TACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATT
CAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGGTTCAGA
AGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTC
AGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGAAGTTTGCAGT
GTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACAC
TACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAA
CGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGT
TAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCA
AAGCTGAATGAGGATCC

Amino Acid Sequence

MYPYDVPDYAEDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDEEEEEEEE
EEDEDEDLEELEVLERKPAAGLSAAPVPTAPAAGAPLMDFGNDFVPPAPR
GPLPAAPPVAPERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLPEDDEPPA
RPPPPPPASVSPQAEPVWTPPAPAPAAPPSTPAAPKRRGSSGSVVVDLLY
WRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGV
IQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLF
LVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQA
QIDHYLGLANKNVKDAMAKIQAKIPGLKRKAE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA tag for mammalian expression