cDNA Clone Nogo b

• Plasmid: pGEX-Nogo b-1
• Parent Plasmid: pGEX6P1
• Genbank: AJ251384
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAAGACCT
GGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGC
CCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAGGAGGAAGAA
GAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGT
GCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAGTGCCCACCG
CCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCG
CCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGCCCCGGAGCG
GCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCAT
CCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCTGAGGACGAC
GAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCA
GGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCGCGCCCCCCT
CCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGTTGTT
GACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGC
CAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAA
CAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGCTTTAGGATA
TACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATT
CAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGGTTCAGA
AGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTC
AGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGAAGTTTGCAGT
GTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACAC
TACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAA
CGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGT
TAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCA
AAGCTGAATGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMYPYDVPDYAEDLDQSP
LVSSSDSPPRPQPAFKYQFVREPEDEEEEEEEEEEDEDEDLEELEVLERK
PAAGLSAAPVPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVAPERQPSW
DPSPVSSTVPAPSPLSAAAVSPSKLPEDDEPPARPPPPPPASVSPQAEPV
WTPPAPAPAAPPSTPAAPKRRGSSGSVVVDLLYWRDIKKTGVVFGASLFL
LLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYL
ESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLKFAVLMWV
FTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAM
AKIQAKIPGLKRKAE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU56
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST/HA tag for bacterial expression