cDNA Clone MYSM1

• Synonyms: 2A-DUB, 2ADUB
• Plasmid: pGEX6P-1-MYSM1 S546L
• Parent Plasmid: pGEX6P1
• Expressed: GST-3C-MYSM1 S546L
• Genbank: NM_001085487.2
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGGCGGCTGAAGAGGCGGATGTGGATATCGAAGGGGACGTGGTAGCGGCGGCGGGGGCACAGCCAGGAAGTGGTGAAAATACAGCATCAGTTTTACAAAAAGATCACTATCTTGATTCATCTTGGAGAACAGAGAATGGCCTTATTCCTTGGACCTTGGATAACACCATCAGTGAAGAGAACAGAGCTGTTATTGAGAAAATGTTGTTGGAAGAAGAATATTATTTATCTAAAAAATCACAACCGGAAAAAGTCTGGCTTGATCAAAAGGAAGATGATAAAAAATACATGAAGAGTCTGCAGAAAACAGCAAAAATCATGGTACACTCTCCTACAAAACCAGCCAGTTACTCAGTAAAGTGGACGATAGAAGAAAAAGAGCTGTTTGAACAAGGGCTGGCTAAATTTGGCCGAAGATGGACCAAAATTTCAAAGCTAATTGGAAGCCGCACTGTTTTACAAGTGAAGAGTTATGCAAGACAGTATTTTAAAAATAAGGTCAAATGCGGTCTGGATAAAGAAACACCAAATCAGAAGACCGGCCATAATCTTCAAGTTAAAAATGAAGATAAAGGGACAAAGGCATGGACACCGTCATGTTTAAGGGGACGTGCTGATCCCAACTTGAATGCTGTAAAAATTGAAAAGTTATCTGATGATGAAGAAGTAGACATCACAGATGAGGTGGACGAGTTGTCTTCTCAAACACCCCAGAAGAATTCTAGCAGTGATCTTTTGTTAGACTTTCCTAATAGTAAAATGCATGAAACCAATCAAGGAGAATTCATTGCTTCTGACAGCCAGGAAGCTCTCTTTTCTAAGTCTTCCAGGGGCTGTCTTCAAAATGAAAAGCAAGATGAAACACTTTCAAGCTCAGAAATTACACTGTGGACTGAGAAACAGAGCAATGGTGACAAAAAATCAATTGAATTAAATGACCAGAAATTTAATGAATTGATTAAAAACTGCAACAAGCATGATGGAAGGGGAATAATAGTTGATGCCAGGCAGTTGCCTTCTCCAGAGCCTTGTGAAATTCAGAAAAATTTGAATGATAATGAAATGCTTTTTCATTCTTGCCAAATGGTAGAGGAAAGCCATGAGGAAGAAGAGCTTAAGCCACCAGAACAGGAAATAGAAATAGATAGAAATATCATTCAAGAAGAAGAAAAACAAGCAATTCCTGAGTTTTTTGAGGGGCGCCAAGCTAAAACACCAGAACGCTATTTGAAAATTAGAAATTATATTTTGGATCAATGGGAGATATGCAAACCGAAATACTTAAATAAGACCTCAGTACGTCCTGGCCTGAAGAACTGTGGAGATGTTAATTGTATTGGACGGATTCATACATACCTCGAATTGATAGGAGCAATCAATTTTGGATGTGAACAGGCTGTGTATAATAGGCCACAAACAGTTGACAAAGTACGAATCAGAGACAGAAAAGATGCAGTAGAAGCATACCAACTTGCCCAGCGTCTGCAGTCTATGCGTACAAGGAGACGTAGGGTCCGAGACCCATGGGGAAACTGGTGTGATGCAAAGGACTTAGAAGGACAAACGTTTGAGCATCTCTCTGCTGAGGAGTTGGCAAAAAGAAGAGAAGAGGAAAAAGGCAGACCTGTTAAATCTTCAAAAGTGCCAAGACCAACAAAAAGCTCGTTTGATCCCTTCCAACTGATACCTTGTAATTTTTTTAGTGAAGAAAAGCAGGAGCCATTTCAGGTGAAAGTGGCTTCAGAAGCACTTTTAATAATGGATTTGCATGCTCATGTTTCTATGGCAGAAGTGATTGGTCTGTTAGGAGGAAGATACTCAGAAGTTGATAAAGTAGTTGAAGTCTGTGCAGCAGAACCATGTAACAGTCTGAGTACAGGACTACAGTGTGAGATGGATCCTGTATCACAAACACAGGCCTCAGAAACCTTGGCTGTTAGAGGCTTCAGTGTTATTGGATGGTATCATTCTCATCCTGCTTTTGATCCTAATCCTTCCTTACGAGATATTGACACACAAGCTAAATACCAGAGTTACTTCTCCAGAGGAGGTGCAAAGTTCATTGGGATGATTGTTAGTCCCTATAATCGAAATAATCCCTTACCATATTCTCAGATTACCTGCCTGGTTATAAGTGAGGAAATTAGCCCAGATGGCTCTTATCGCTTACCTTACAAATTTGAAGTACAGCAGATGTTAGAAGAACCTCAGTGGGGATTAGTATTTGAAAAGACAAGATGGATAATAGAAAAATACAGGCTCTCCCATAGCAGCGTCCCCATGGATAAAATCTTTCGCCGGGATTCTGACCTGACTTGTTTGCAGAAACTTTTGGAGTGTATGAGGAAGACTCTGAGCAAAGTGACCAATTGCTTTATGGCTGAAGAATTCTTGACTGAAATAGAAAATTTGTTCCTTTCCAATTATAAAAGCAACCAAGAGAATGGAGTAACCGAAGAGAACTGTACAAAGGAATTGTTAATGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAEEADVDIEGDVVAAAGAQPGSGENTASVLQKDHYLDSSWRTENGLIPWTLDNTISEENRAVIEKML
LEEEYYLSKKSQPEKVWLDQKEDDKKYMKSLQKTAKIMVHSPTKPASYSVKWTIEEKELFEQGLAKFGRRWTKISKLIGSRTVLQVKSYARQYFKNKVKC
GLDKETPNQKTGHNLQVKNEDKGTKAWTPSCLRGRADPNLNAVKIEKLSDDEEVDITDEVDELSSQTPQKNSSSDLLLDFPNSKMHETNQGEFIASDSQE
ALFSKSSRGCLQNEKQDETLSSSEITLWTEKQSNGDKKSIELNDQKFNELIKNCNKHDGRGIIVDARQLPSPEPCEIQKNLNDNEMLFHSCQMVEESHEE
EELKPPEQEIEIDRNIIQEEEKQAIPEFFEGRQAKTPERYLKIRNYILDQWEICKPKYLNKTSVRPGLKNCGDVNCIGRIHTYLELIGAINFGCEQAVYN
RPQTVDKVRIRDRKDAVEAYQLAQRLQSMRTRRRRVRDPWGNWCDAKDLEGQTFEHLSAEELAKRREEEKGRPVKSSKVPRPTKSSFDPFQLIPCNFFSE
EKQEPFQVKVASEALLIMDLHAHVSMAEVIGLLGGRYSEVDKVVEVCAAEPCNSLSTGLQCEMDPVSQTQASETLAVRGFSVIGWYHSHPAFDPNPSLRD
IDTQAKYQSYFSRGGAKFIGMIVSPYNRNNPLPYSQITCLVISEEISPDGSYRLPYKFEVQQMLEEPQWGLVFEKTRWIIEKYRLSHSSVPMDKIFRRDS
DLTCLQKLLECMRKTLSKVTNCFMAEEFLTEIENLFLSNYKSNQENGVTEENCTKELLM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU33915
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: L546S. Also contains the benign variation T264A and 3 silent nucleotide changes; A>C 594, C>T 735 and A>G 1275 bp.