cDNA Clone DGKZ

• Plasmid: pGEX6P-DGKz Q542/K
• Parent Plasmid: pGEX6P1
• Expressed: GST-DGKz Q542/K
• Genbank: AE004091.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCATGAACAGTACCAGGCTCCGCTTCCCGTGAATTCCCCAGCGCTTCCCGAACCCTTCTACTACCTGCACAACTTCCGCGCGGTGCTCGCCTGGATCGGCGAACGCTATGCCGATCTGCTCGACGATCAGGAACGTGCCTTCATCGCCGCGTTCGCCGAGCTGCCCGAGGCCAGCCAGGCCCTGCTGGTGCGCATGGTGATGCGCAAGGGCACGCTGTTCCGCGAAGGCAAGCTGGCCTATGCCGAGATCGGCGACACGCGCGCCGCCGTGCAACCGCTGTTGGCATTGGGCTGGGTGGACGCGCAGCCGACCCTGGAACTGGCGCAACTGTTCGGCTTGCTGAAGAAGGACGAATTGTCGCAGCTGTTCAGGGACCATCTCGGGCGCGCGAACCTGCGCAAGGATGCGCTGCTGGAACGGCTCCAGCCGTTGTTCCCCGAGGCGCGGCGGCTCGCCGAGTGGCAGGCCGACTTCGCCGAACCGGTCTACGAGTTGCGCTGCATGGCGCTCTGCGACCGCTTGCGGTTGATGTATTTCGGCAACCTGTGGCAGGACTGGTCGGAGTTCGTCCTTGCCGACCTGGGTATCTATCGTTACGAAAGCGTCGAGTTTTCCGCCGACTCTCGCGGCTTTCGCCTGCGCGCCGACGTCGACGCCTATCTCCACCTGTTCGACTGCCGCCAGCGCTTCGACCTCGGCGAGCCGCTGGAGGAACTGCTCGCCGGCCTGCCGGGCGAACCCTACGCCAATCCCTGGCTGGAAGGGCGGCGGGTCAAGCTGCTGTTCCAGTTCGCCCAGCACTGCGAAAAGCAGCGGGATTTCGACCTGGCCCAGCGGCTCTATCGCCAATCCAGCCACCCCGGCGCGCGCCTGCGGGCGATTCGCAGCCTGGAACGCGGCGAGCGCTTCGCCGAAGCCCATGCATTGGCGCGGGAGGCCAGCTGTGCGCCGGAAAGCGACGCCGAACGCCAGGGCCTGGCCCGATTGCTGCCGCGCCTGCAAGGCAAGCTCGGCCTGCCGCGACAGGCGCGGGCGGCGGCTCCGGAAATCGACCGCCTGGACCTGTGCCTGGCGTTCCCGAGCGAACCCTGTAGCGTCGAGTGGGCGGTCCGCGAGCACCTGGAAGAGCCCGGTTGCGCGGTGCACTACGTCGAGAACGGCCTGATCAATTCGCTGTTCGGGCTGCTCTGCTGGGAGGCGATCTTCGCCGCCATTCCCGGGGCGTTCTTCCATCCCTTCCACAGCGCGCCGGCGGACCTGCACAGCGCCGACTTCCGCCAGCGCCGCGCCGCCCTGTTCGAGGCCTGCCTGGGACGGCTGGAGGACGGCAGCTACCGCGACGCGATCCGCTGTCGCTACCGCGACAAGTTCGGCCTGCAGTCGCCCTTCGTCTACTGGGAACTGCTTGGCGAGGAACTGCTCGAACAGGCGCTCGATTGCCTGCCGGCGGCCCACCTGCGCGCCTGGTTCGAACGCCTGCTGGAGGATATCCCGGGCAACCGCGCCGGCCTGCCGGACCTGATCCAGTTCTGGCCGGCGCAGCGGCGCTACCGGATGGTCGAGGTCAAGGGACCGGGCGACCGCCTGCAGGACAACCAGTTGCGCTGGCTGCAATTCTGCCGCGAGCGGGAGATGCCGGTGGCGGTCTGCTACGTGCGCTGGCATGTCGATGACTGAGCGGCCGC

Amino Acid Sequence

MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGARGSMHEQYQAPLPVNSPALPEPFYYLHNFRAVLAWIGERYADLLDDQERAFIAAFAELPEASQALLVRMVMRKGTLFREGKLAYAEIGDTRAAVQPLLALGWVDAQPTLELAQLFGLLKKDELSQLFRDHLGRANLRKDALLERLQPLFPEARRLAEWQADFAEPVYELRCMALCDRLRLMYFGNLWQDWSEFVLADLGIYRYESVEFSADSRGFRLRADVDAYLHLFDCRQRFDLGEPLEELLAGLPGEPYANPWLEGRRVKLLFQFAQHCEKQRDFDLAQRLYRQSSHPGARLRAIRSLERGERFAEAHALAREASCAPESDAERQGLARLLPRLQGKLGLPRQARAAAPEIDRLDLCLAFPSEPCSVEWAVREHLEEPGCAVHYVENGLINSLFGLLCWEAIFAAIPGAFFHPFHSAPADLHSADFRQRRAALFEACLGRLEDGSYRDAIRCRYRDKFGLQSPFVYWELLGEELLEQALDCLPAAHLRAWFERLLEDIPGNRAGLPDLIQFWPAQRRYRMVEVKGPGDRLQDNQLRWLQFCREREMPVAVCYVRWHVDD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU33706
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N-ter NUS tag to aid solubility; ; Estimated MW of expressed protein : 125286.21 Daltons