cDNA Clone FAN1 (Pseudomonas)

• Plasmid: pGEX6P-1-FAN1p
• Parent Plasmid: pGEX6P1
• Expressed: GST-FAN1p
• Genbank: AE004091.2
• Tag: GST
• Species: Pseudomonas

Sequence of Insert

GGATCCATGCATGAACAGTACCAGGCTCCGCTTCCCGTGAATTCCCCAGCGCTTCCCGAACCCTTCTACTACCTGCACAACTTCCGCGCGGTGCTCGCCTGGATCGGCGAACGCTATGCCGATCTGCTCGACGATCAGGAACGTGCCTTCATCGCCGCGTTCGCCGAGCTGCCCGAGGCCAGCCAGGCCCTGCTGGTGCGCATGGTGATGCGCAAGGGCACGCTGTTCCGCGAAGGCAAGCTGGCCTATGCCGAGATCGGCGACACGCGCGCCGCCGTGCAACCGCTGTTGGCATTGGGCTGGGTGGACGCGCAGCCGACCCTGGAACTGGCGCAACTGTTCGGCTTGCTGAAGAAGGACGAATTGTCGCAGCTGTTCAGGGACCATCTCGGGCGCGCGAACCTGCGCAAGGATGCGCTGCTGGAACGGCTCCAGCCGTTGTTCCCCGAGGCGCGGCGGCTCGCCGAGTGGCAGGCCGACTTCGCCGAACCGGTCTACGAGTTGCGCTGCATGGCGCTCTGCGACCGCTTGCGGTTGATGTATTTCGGCAACCTGTGGCAGGACTGGTCGGAGTTCGTCCTTGCCGACCTGGGTATCTATCGTTACGAAAGCGTCGAGTTTTCCGCCGACTCTCGCGGCTTTCGCCTGCGCGCCGACGTCGACGCCTATCTCCACCTGTTCGACTGCCGCCAGCGCTTCGACCTCGGCGAGCCGCTGGAGGAACTGCTCGCCGGCCTGCCGGGCGAACCCTACGCCAATCCCTGGCTGGAAGGGCGGCGGGTCAAGCTGCTGTTCCAGTTCGCCCAGCACTGCGAAAAGCAGCGGGATTTCGACCTGGCCCAGCGGCTCTATCGCCAATCCAGCCACCCCGGCGCGCGCCTGCGGGCGATTCGCAGCCTGGAACGCGGCGAGCGCTTCGCCGAAGCCCATGCATTGGCGCGGGAGGCCAGCTGTGCGCCGGAAAGCGACGCCGAACGCCAGGGCCTGGCCCGATTGCTGCCGCGCCTGCAAGGCAAGCTCGGCCTGCCGCGACAGGCGCGGGCGGCGGCTCCGGAAATCGACCGCCTGGACCTGTGCCTGGCGTTCCCGAGCGAACCCTGTAGCGTCGAGTGGGCGGTCCGCGAGCACCTGGAAGAGCCCGGTTGCGCGGTGCACTACGTCGAGAACGGCCTGATCAATTCGCTGTTCGGGCTGCTCTGCTGGGAGGCGATCTTCGCCGCCATTCCCGGGGCGTTCTTCCATCCCTTCCACAGCGCGCCGGCGGACCTGCACAGCGCCGACTTCCGCCAGCGCCGCGCCGCCCTGTTCGAGGCCTGCCTGGGACGGCTGGAGGACGGCAGCTACCGCGACGCGATCCGCTGTCGCTACCGCGACAAGTTCGGCCTGCAGTCGCCCTTCGTCTACTGGGAACTGCTTGGCGAGGAACTGCTCGAACAGGCGCTCGATTGCCTGCCGGCGGCCCACCTGCGCGCCTGGTTCGAACGCCTGCTGGAGGATATCCCGGGCAACCGCGCCGGCCTGCCGGACCTGATCCAGTTCTGGCCGGCGCAGCGGCGCTACCGGATGGTCGAGGTCAAGGGACCGGGCGACCGCCTGCAGGACAACCAGTTGCGCTGGCTGCAATTCTGCCGCGAGCGGGAGATGCCGGTGGCGGTCTGCTACGTGCGCTGGCATGTCGATGACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMHEQYQAPLPVNSPALPEPFYYLHNFRAVLAWIGERYADLLDDQERAFIAAFAELPEASQALLVRMVMRKGTLFREGKLAYAEIGDTRAAVQPLLALGWVDAQPTLELAQLFGLLKKDELSQLFRDHLGRANLRKDALLERLQPLFPEARRLAEWQADFAEPVYELRCMALCDRLRLMYFGNLWQDWSEFVLADLGIYRYESVEFSADSRGFRLRADVDAYLHLFDCRQRFDLGEPLEELLAGLPGEPYANPWLEGRRVKLLFQFAQHCEKQRDFDLAQRLYRQSSHPGARLRAIRSLERGERFAEAHALAREASCAPESDAERQGLARLLPRLQGKLGLPRQARAAAPEIDRLDLCLAFPSEPCSVEWAVREHLEEPGCAVHYVENGLINSLFGLLCWEAIFAAIPGAFFHPFHSAPADLHSADFRQRRAALFEACLGRLEDGSYRDAIRCRYRDKFGLQSPFVYWELLGEELLEQALDCLPAAHLRAWFERLLEDIPGNRAGLPDLIQFWPAQRRYRMVEVKGPGDRLQDNQLRWLQFCREREMPVAVCYVRWHVDD

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU33174
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 91352.19 Daltons; ; All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.