cDNA Clone ATXN3

• Plasmid: pGEX6P-1-ATXN3
• Parent Plasmid: pGEX6P1
• Expressed: GST ATXN3
• Genbank: AAH33711.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGTCCATCTTCCACGAGAAACAAGAAGGCTCACTTTGTGC
TCAACATTGCCTGAATAACTTATTGCAAGGAGAATATTTTAGCCCTGTGG
AATTATCCTCAATTGCACATCAGCTGGATGAGGAGGAGAGGATGAGAATG
GCAGAAGGAGGAGTTACTAGTGAAGATTATCGCACGTTTTTACAGCAGCC
TTCTGGAAATATGGATGACAGTGGTTTTTTCTCTATTCAGGTTATAAGCA
ATGCCTTGAAAGTTTGGGGTTTAGAACTAATCCTGTTCAACAGTCCAGAG
TATCAGAGGCTCAGGATCGATCCTATAAATGAAAGATCATTTATATGCAA
TTATAAGGAACACTGGTTTACAGTTAGAAAATTAGGAAAACAGTGGTTTA
ACTTGAATTCTCTCTTGACGGGTCCAGAATTAATATCAGATACATATCTT
GCACTTTTCTTGGCTCAATTACAACAGGAAGGTTATTCTATATTTGTCGT
TAAGGGTGATCTGCCAGATTGCGAAGCTGACCAACTCCTGCAGATGATTA
GGGTCCAACAGATGCATCGACCAAAACTTATTGGAGAAGAATTAGCACAA
CTAAAAGAGCAAAGAGTCCATAAAACAGACCTGGAACGAGTGTTAGAAGC
AAATGATGGCTCAGGAATGTTAGACGAAGATGAGGAGGATTTGCAGAGGG
CTCTGGCACTAAGTCGCCAAGAAATTGACATGGAAGATGAGGAAGCAGAT
CTCCGCAGGGCTATTCAGCTAAGTATGCAAGGTAGTTCCAGAAACATATC
TCAAGATATGACACAGACATCAGGTACAAATCTTACTTCAGAAGAGCTTC
GGAAGAGACGAGAAGCCTACTTTGAAAAACAGCAGCAAAAGCAGCAACAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGG
GGACCTATCAGGACAGAGTTCACATCCATGTGAAAGGCCAGCCACCAGTT
CAGGAGCACTTGGGAGTGATCTAGGTGATGCTATGAGTGAAGAAGACATG
CTTCAGGCAGCTGTGACCATGTCTTTAGAAACTGTCAGAAATGATTTGAA
AACAGAAGGAAAAAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESIFHEKQEGSLCAQHCL
NNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNM
DDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEH
WFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDL
PDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGS
GMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMT
QTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSG
QSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGK
K*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35448
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 69225 Daltons