cDNA Clone MSK1

• Synonyms: RPS6KA5
• Plasmid: pET156P MSK1
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His MSK1
• Genbank: AF074393
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTATGGAGGAGGAGGGTGGCAGCAGCGGCGGCGCCGCGGGGACCAG
CGCGGACGGCGGCGACGGAGGAGAGCAGCTCCTCACTGTCAAGCACGAGC
TGCGGACTGCTAATTTGACAGGACATGCTGAGAAGGTGGGAATAGAAAAT
TTTGAGCTCCTGAAGGTCCTAGGAACTGGAGCTTATGGAAAAGTATTTCT
AGTTCGTAAAATAAGTGGCCATGATACTGGAAAGCTGTATGCCATGAAAG
TTTTGAAAAAGGCAACAATCGTTCAAAAGGCCAAAACCACAGAGCATACA
AGGACAGAACGACAAGTCCTGGAACACATTAGGCAGTCGCCATTTTTGGT
AACATTACATTATGCTTTCCAGACAGAAACCAAACTTCATCTCATTTTAG
ATTATATAAATGGTGGTGAACTTTTTACTCATCTTTCTCAAAGAGAGCGT
TTCACAGAGCATGAGGTGCAGATTTATGTTGGAGAGATTGTGCTTGCCCT
CGAACATCTCCACAAGTTGGGGATTATATATCGTGATATTAAGCTTGAGA
ATATTCTACTTGATTCTAATGGCCATGTGGTGCTGACAGATTTTGGTCTG
AGTAAGGAGTTTGTGGCTGATGAAACTGAAAGAGCATATTCCTTTTGTGG
AACTATTGAATACATGGCACCAGATATTGTCAGAGGGGGAGATTCAGGAC
ATGACAAGGCAGTTGACTGGTGGAGTTTGGGTGTTCTAATGTATGAATTA
CTAACTGGAGCATCTCCTTTCACTGTTGATGGAGAAAAAAATTCCCAAGC
TGAGATATCTAGGAGAATATTAAAAAGTGAGCCTCCATATCCCCAAGAAA
TGAGTGCTTTAGCGAAAGACCTAATTCAGCGTCTTTTGATGAAAGATCCC
AAGAAGAGATTGGGATGTGGTCCACGTGATGCAGATGAAATCAAAGAACA
TCTCTTCTTTCAGAAAATAAATTGGGATGATTTAGCCGCCAAAAAAGTGC
CTGCACCATTTAAGCCAGTCATTCGAGATGAATTAGATGTGAGTAACTTT
GCAGAAGAGTTCACAGAAATGGATCCCACTTATTCTCCCGCAGCCCTGCC
CCAGAGTTCTGAGAAGCTGTTTCAGGGCTATTCCTTTGTTGCTCCTTCCA
TCCTATTCAAGCGTAATGCAGCTGTCATAGACCCTCTTCAGTTTCACATG
GGAGTTGAACGTCCTGGAGTGACAAATGTTGCCAGGAGTGCAATGATGAA
GGACTCTCCATTCTATCAACACTATGACCTAGATTTGAAGGACAAACCCC
TGGGAGAAGGTAGTTTTTCAATTTGTCGAAAGTGTGTGCATAAAAAAAGT
AACCAAGCTTTTGCAGTCAAAATAATCAGCAAAAGGATGGAAGCCAATAC
TCAAAAGGAAATAACAGCTCTGAAACTCTGTGAAGGACACCCCAATATTG
TGAAGTTGCATGAAGTTTTTCATGATCAGCTTCACACGTTTCTAGTGATG
GAACTTCTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAAAGAAGCA
CTTCAGTGAGACGGAAGCCAGCTACATCATGAGGAAGCTTGTTTCAGCTG
TAAGCCACATGCATGATGTTGGAGTGGTGCACAGGGATCTGAAACCTGAG
AATTTATTGTTCACCGATGAAAATGACAATTTGGAAATTAAAATAATTGA
TTTTGGATTTGCACGGCTAAAGCCACCGGATAATCAGCCCCTGAAGACTC
CATGCTTCACCCTTCATTATGCCGCCCCAGAGCTCTTGAATCAGAACGGC
TACGATGAGTCCTGTGACCTGTGGAGCTTGGGCGTCATTTTGTACACAAT
GTTGTCAGGACAGGTTCCCTTCCAATCTCATGACCGAAGTTTGACGTGTA
CCAGCGCGGTGGAAATCATGAAGAAAATTAAAAAGGGAGATTTCTCCTTT
GAAGGAGAAGCCTGGAAGAATGTATCCCAAGAGGCTAAAGATTTGATCCA
AGGACTTCTCACAGTAGATCCAAACAAAAGGCTTAAAATGTCTGGCTTGA
GGTACAATGAATGGCTACAAGATGGAAGTCAGCTGTCCTCCAATCCTCTG
ATGACTCCGGATATTCTAGGATCTTCCGGAGCTGCCGTGCATACCTGTGT
GAAAGCAACCTTCCACGCCTTTAACAAATACAAGAGAGAGGGGTTTTGCC
TTCAGAATGTTGATAAGGCCCCTTTGGCTAAGAGAAGAAAAATGAAAAAG
ACTAGCACCAGTACCGAGACGCGCAGCAGTTCCAGTGAGAGTTCCCATTC
TTCTTCCTCTCATTCTCACGGTAAAACTACACCCACCAAGACACTGCAGC
CCAGCAATCCTGCCGACAGCAATAACCCGGAGACCCTCTTCCAGTTCTCG
GACTCAGTAGCTTAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMEEEGGSSGGAAGTSADGGDGGEQLLT
VKHELRTANLTGHAEKVGIENFELLKVLGTGAYGKVFLVRKISGHDTGKL
YAMKVLKKATIVQKAKTTEHTRTERQVLEHIRQSPFLVTLHYAFQTETKL
HLILDYINGGELFTHLSQRERFTEHEVQIYVGEIVLALEHLHKLGIIYRD
IKLENILLDSNGHVVLTDFGLSKEFVADETERAYSFCGTIEYMAPDIVRG
GDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAEISRRILKSEPP
YPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFFQKINWDDLA
AKKVPAPFKPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSEKLFQGYSF
VAPSILFKRNAAVIDPLQFHMGVERPGVTNVARSAMMKDSPFYQHYDLDL
KDKPLGEGSFSICRKCVHKKSNQAFAVKIISKRMEANTQKEITALKLCEG
HPNIVKLHEVFHDQLHTFLVMELLNGGELFERIKKKKHFSETEASYIMRK
LVSAVSHMHDVGVVHRDLKPENLLFTDENDNLEIKIIDFGFARLKPPDNQ
PLKTPCFTLHYAAPELLNQNGYDESCDLWSLGVILYTMLSGQVPFQSHDR
SLTCTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDLIQGLLTVDPNKRLK
MSGLRYNEWLQDGSQLSSNPLMTPDILGSSGAAVHTCVKATFHAFNKYKR
EGFCLQNVDKAPLAKRRKMKKTSTSTETRSSSSESSHSSSSHSHGKTTPT
KTLQPSNPADSNNPETLFQFSDSVA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35143
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression; ; Estimated MW of expressed protein : 92307 Daltons