cDNA Clone NEF

• Plasmid: pCMV-HA-1 NEF
• Parent Plasmid: pCMV5 HA1
• Expressed: HA NEF
• Genbank: AAD01451.1
• Tag: HA
• Species: HIV-1

Sequence of Insert

GGATCCATGGGTGGCAAGTGGTCAAAAAGTAGTGTGATTGGATGGCCTAC
TGTAAGGGAAAGAATGAGACGAGCTGAGCCAGCAGCAGATAGGGTGGGAG
CAGCATCTCGAGACCTGGAAAAACATGGAGCAATCACAAGTAGCAATACA
GCAGCTACCAATGCTGCTTGTGCCTGGCTAGAAGCACAAGAGGAGGAGGA
GGTGGGTTTTCCAGTCACACCTCAGGTACCTTTAAGACCAATGACTTACA
AGGCAGCTGTAGATCTTAGCCACTTTTTAAAAGAAAAGGGGGGACTGGAA
GGGCTAATTCACTCCCAAAGAAGACAAGATATCCTTGATCTGTGGATCTA
CCACACACAAGGCTACTTCCCTGATTAGCAGAACTACACACCAGGGCCAG
GGGTCAGATATCCACTGACCTTTGGATGGTGCTACAAGCTAGTACCAGTT
GAGCCAGATAAGATAGAAGAGGCCAATAAAGGAGAGAACACCAGCTTGTT
ACACCCTGTGAGCCTGCATGGGATGGATGACCCGGAGAGAGAAGTGTTAG
AGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACGTGGCCCGAGAGCTG
CATCCGGAGTACTTCAAGAACTGCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMGGKWSKSSVIGWPTVRERMRRAEPAADRVGAASRDLE
KHGAITSSNTAATNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLS
HFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPD*QNYTPGPGVRYPLT
FGWCYKLVPVEPDKIEEANKGENTSLLHPVSLHGMDDPEREVLEWRFDSR
LAFHHVARELHPEYFKNC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32969
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression; ; Estimated MW of expressed protein : 24641 Daltons