cDNA Clone HDAC5

• Plasmid: pGEX6P-1-HDAC5
• Parent Plasmid: pGEX6P1
• Expressed: GST HDAC5
• Genbank: NM_005474.4
• Tag: DAC
• Species: Human

Sequence of Insert

AGATCTATGAACTCTCCCAACGAGTCGGATGGGATGTCAGGTCGGGAACC
ATCCTTGGAAATCCTGCCGCGGACTTCTCTGCACAGCATCCCTGTGACAG
TGGAGGTGAAGCCGGTGCTGCCAAGAGCCATGCCCAGTTCCATGGGGGGT
GGGGGTGGAGGCAGCCCCAGCCCTGTGGAGCTACGGGGGGCTCTGGTGGG
CTCTGTGGACCCCACACTGCGGGAGCAGCAACTGCAGCAGGAGCTCCTGG
CGCTCAAGCAGCAGCAGCAGCTGCAGAAGCAGCTCCTGTTCGCTGAGTTC
CAGAAACAGCATGACCACCTGACAAGGCAGCATGAGGTCCAGCTGCAGAA
GCACCTCAAGCAGCAGCAGGAGATGCTGGCAGCCAAGCAGCAGCAGGAGA
TGCTGGCAGCCAAGCGGCAGCAGGAGCTGGAGCAGCAGCGGCAGCGGGAG
CAGCAGCGGCAGGAAGAGCTGGAGAAGCAGCGGCTGGAGCAGCAGCTGCT
CATCCTGCGGAACAAGGAGAAGAGCAAAGAGAGTGCCATTGCCAGCACTG
AGGTAAAGCTGAGGCTCCAGGAATTCCTCTTGTCGAAGTCAAAGGAGCCC
ACACCAGGCGGCCTCAACCATTCCCTCCCACAGCACCCCAAATGCTGGGG
AGCCCACCATGCTTCTTTGGACCAGAGTTCCCCTCCCCAGAGCGGCCCCC
CTGGGACGCCTCCCTCCTACAAACTGCCTTTGCCTGGGCCCTACGACAGT
CGAGACGACTTCCCCCTCCGCAAAACAGCCTCTGAACCCAACTTGAAAGT
GCGTTCAAGGCTAAAACAGAAGGTGGCTGAGCGGAGAAGCAGTCCCCTCC
TGCGTCGCAAGGATGGGACTGTTATTAGCACCTTTAAGAAGAGAGCTGTT
GAGATCACAGGTGCCGGGCCTGGGGCGTCGTCCGTGTGTAACAGCGCACC
CGGCTCCGGCCCCAGCTCTCCCAACAGCTCCCACAGCACCATCGCTGAGA
ATGGCTTTACTGGCTCAGTCCCCAACATCCCCACTGAGATGCTCCCTCAG
CACCGAGCCCTCCCTCTGGACAGCTCCCCCAACCAGTTCAGCCTCTACAC
GTCTCCTTCTCTGCCCAACATCTCCCTAGGGCTGCAGGCCACGGTCACTG
TCACCAACTCACACCTCACTGCCTCCCCGAAGCTGTCGACACAGCAGGAG
GCCGAGAGGCAGGCCCTCCAGTCCCTGCGGCAGGGTGGCACGCTGACCGG
CAAGTTCATGAGCACATCCTCTATTCCTGGCTGCCTGCTGGGCGTGGCAC
TGGAGGGCGACGGGAGCCCCCACGGGCATGCCTCCCTGCTGCAGCATGTG
CTGTTGCTGGAGCAGGCCCGGCAGCAGAGCACCCTCATTGCTGTGCCACT
CCACGGGCAGTCCCCACTAGTGACGGGTGAACGTGTGGCCACCAGCATGC
GGACGGTAGGCAAGCTCCCGCGGCATCGGCCCCTGAGCCGCACTCAGTCC
TCACCGCTGCCGCAGAGTCCCCAGGCCCTGCAGCAGCTGGTCATGCAACA
ACAGCACCAGCAGTTCCTGGAGAAGCAGAAGCAGCAGCAGCTACAGCTGG
GCAAGATCCTCACCAAGACAGGGGAGCTGCCCAGGCAGCCCACCACCCAC
CCTGAGGAGACAGAGGAGGAGCTGACGGAGCAGCAGGAGGTCTTGCTGGG
GGAGGGAGCCCTGACCATGCCCCGGGAGGGCTCCACAGAGAGTGAGAGCA
CACAGGAAGACCTGGAGGAGGAGGACGAGGAAGACGATGGGGAGGAGGAG
GAGGATTGCATCCAGGTTAAGGACGAGGAGGGCGAGAGTGGTGCTGAGGA
GGGGCCCGACTTGGAGGAGCCTGGTGCTGGATACAAAAAACTGTTCTCAG
ATGCCCAGCCGCTGCAGCCTTTGCAGGTGTACCAGGCGCCCCTCAGCCTG
GCCACTGTGCCCCACCAGGCCCTGGGCCGTACCCAGTCCTCCCCTGCTGC
CCCTGGGGGCATGAAGAGCCCCCCAGACCAGCCCGTCAAGCACCTCTTCA
CCACAGGTGTGGTCTACGACACGTTCATGCTAAAGCACCAGTGCATGTGC
GGGAACACACACGTGCACCCTGAGCATGCTGGCCGGATCCAGAGCATCTG
GTCCCGGCTGCAGGAGACAGGCCTGCTTAGCAAGTGCGAGCGGATCCGAG
GTCGCAAAGCCACGCTAGATGAGATCCAGACAGTGCACTCTGAATACCAC
ACCCTGCTCTATGGGACCAGTCCCCTCAACCGGCAGAAGCTAGACAGCAA
GAAGTTGCTCGGCCCCATCAGCCAGAAGATGTATGCTGTGCTGCCTTGTG
GGGGCATCGGGGTGGACAGTGACACCGTGTGGAATGAGATGCACTCCTCC
AGTGCTGTGCGCATGGCAGTGGGCTGCCTGCTGGAGCTGGCCTTCAAGGT
GGCTGCAGGAGAGCTCAAGAATGGATTTGCCATCATCCGGCCCCCAGGAC
ACCACGCCGAGGAATCCACAGCCATGGGATTCTGCTTCTTCAACTCTGTA
GCCATCACCGCAAAACTCCTACAGCAGAAGTTGAACGTGGGCAAGGTCCT
CATCGTGGACTGGGACATTCACCATGGCAATGGCACCCAGCAGGCGTTCT
ATAATGACCCCTCTGTGCTCTACATCTCTCTGCATCGCTATGACAACGGG
AACTTCTTTCCAGGCTCTGGGGCTCCTGAAGAGGTTGGTGGAGGACCAGG
CGTGGGGTACAATGTGAACGTGGCATGGACAGGAGGTGTGGACCCCCCCA
TTGGAGACGTGGAGTACCTTACAGCCTTCAGGACAGTGGTGATGCCCATT
GCCCACGAGTTCTCACCTGATGTGGTCCTAGTCTCCGCCGGGTTTGATGC
TGTTGAAGGACATCTGTCTCCTCTGGGTGGCTACTCTGTCACCGCCAGAT
GTTTTGGCCACTTGACCAGGCAGCTGATGACCCTGGCAGGGGGCCGGGTG
GTGCTGGCCCTGGAGGGAGGCCATGACTTGACCGCCATCTGTGATGCCTC
TGAGGCTTGTGTCTCGGCTCTGCTCAGTGTAGAGCTGCAGCCCTTGGATG
AGGCAGTCTTGCAGCAAAAGCCCAACATCAACGCAGTGGCCACGCTAGAG
AAAGTCATCGAGATCCAGAGCAAACACTGGAGCTGTGTGCAGAAGTTCGC
CGCTGGTCTGGGCCGGTCCCTGCGAGAGGCCCAAGCAGGTGAGACCGAGG
AGGCCGAGACTGTGAGCGCCATGGCCTTGCTGTCGGTGGGGGCCGAGCAG
GCCCAGGCTGCGGCAGCCCGGGAACACAGCCCCAGGCCGGCAGAGGAGCC
CATGGAGCAGGAGCCTGCCCTGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNSPNESDGMSGREPSLEI
LPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDP
TLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQ
QQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRN
KEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHA
SLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRL
KQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGP
SSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSL
PNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMS
TSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQS
PLVTGERVATSMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQ
FLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQEVLLGEGAL
TMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDL
EEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGM
KSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQ
ETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLG
PISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGE
LKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDW
DIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYN
VNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGH
LSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACV
SALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLG
RSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQE
PAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32746
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 148796 Daltons