cDNA Clone IKK alpha

• Plasmid: pEBG6P-IKK alpha S176A S180A
• Parent Plasmid: pEBG6P
• Expressed: GST IKK alpha S176A S180A
• Genbank: O15111
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCC
CTGGGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTCGGGAACGTCTGTC
TGTACCAGCATCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCGC
CTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCATGAAATCCAGAT
TATGAAGAAGTTGAACCATGCCAATGTTGTAAAGGCCTGTGATGTTCCTG
AAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAGCAATGGAATAC
TGTTCTGGAGGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTG
TGGACTTAAAGAAAGCCAGATACTTTCTTTACTAAGTGATATAGGGTCTG
GGATTCGATATTTGCATGAAAACAAAATTATACATCGAGATCTAAAACCT
GAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATACATAAAATAAT
TGATCTGGGATATGCCAAAGATGTTGATCAAGGAGCTCTGTGTACAGCTT
TTGTGGGAACACTGCAGTATCTGGCCCCAGAGCTCTTTGAGAATAAGCCT
TACACAGCCACTGTTGATTATTGGAGCTTTGGGACCATGGTATTTGAATG
TATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGC
ATGAGAAGATTAAGAAGAAGGATCCAAAGTGTATATTTGCATGTGAAGAG
ATGTCAGGAGAAGTTCGGTTTAGTAGCCATTTACCTCAACCAAATAGCCT
TTGTAGTTTAATAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTGA
ATTGCGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAG
CCAAGATGTTTTGTATTAATGGATCACATTTTGAATTTGAAGATAGTACA
CATCCTAAATATGACTTCTGCAAAGATAATTTCTTTTCTGTTACCACCTG
ATGAAAGTCTTCATTCACTACAGTCTCGTATTGAGCGTGAAACTGGAATA
AATACTGGTTCTCAAGAACTTCTTTCAGAGACAGGAATTTCTCTGGATCC
TCGGAAACCAGCCTCTCAATGTGTTCTAGATGGAGTTAGAGGCTGTGATA
GCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCCA
TTTGCTTCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAG
CAAAATACAGCTTCCAATTATACAGCTGCGTAAAGTGTGGGCTGAAGCAG
TGCACTATGTGTCTGGACTAAAAGAAGACTATAGCAGGCTCTTTCAGGGA
CAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTAACAAA
AATGAAGAACACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTGG
AGTTTTTTCACAAAAGCATTCAGCTTGACTTGGAGAGATACAGCGAGCAG
ATGACGTATGGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAAT
GGAAGAAAAGGCCATCCACTATGCTGAGGTTGGTGTCATTGGATACCTGG
AGGATCAGATTATGTCTTTGCATGCTGAAATCATGGAGCTACAGAAGAGC
CCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTGGAACAGCGTGC
CATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTACA
GTGACAGCACAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAG
GACCGTGTGCTCAAGGAGCTGTTTGGTCATTTGAGCAAGTTGTTGGGCTG
TAAGCAGAAGATTATTGATCTACTCCCTAAGGTGGAAGTGGCCCTCAGTA
ATATCAAAGAAGCTGACAATACTGTCATGTTCATGCAGGGGAAAAGGCAG
AAAGAAATATGGCATCTCCTTAAAATTGCATGTACACAGAGTTCTGCCCG
GTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCCTCAGACATCAG
CATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTG
GTAACTCCTCAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTT
GAACTGCCTTGGCCATTTAAGCACTATTATTCATGAGGCAAATGAGGAAC
AGGGCAATAGTATGATGAATCTTGATTGGAGTTGGTTAACAGAATGAGCG
GCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMERP
PGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKN
RERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGDLR
KLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQD
VGGKIIHKIIDLGYAKDVDQGALCTAFVGTLQYLAPELFENKPYTATVDY
WSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRF
SSHLPQPNSLCSLIVEPMENWLQLMLNCDPQQRGGPVDLTLKQPRCFVLM
DHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQEL
LSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLS
DCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLS
LLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISS
EKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQG
DLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKEL
FGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLL
KIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGE
TSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32701
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 112741 Daltons