cDNA Clone MAP4K4

• Synonyms: HGK
• Plasmid: pEBG6P-MAP4K4
• Parent Plasmid: pEBG6P
• Expressed: GST MAP4K4
• Genbank: EAX01810.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGA
CCTCTCCTCCCTGCGGGATCCTGCTGGGATTTTTGAGCTGGTGGAAGTGG
TTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACG
GGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGA
AGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAA
ACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACAT
GATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTAC
AGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCG
CTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCAT
CATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAA
TGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGA
CTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTGGATGGCTCCT
GAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAG
TGATCTTTGGTCTTGTGGCATTACAGCCATTGAGATGGCAGAAGGTGCTC
CCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGA
AACCCTCCTCCCCGGCTGAAGTCAAAAAAATGGTCGAAGAAGTTTTTTAG
TTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAG
AGCAGCTTTTGAAACATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAA
GTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGG
CGAGAAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGG
AGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCT
GGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAA
GGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGC
TCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAG
CGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAG
GAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAG
AACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAA
GAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGA
ACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAG
TCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCATGACCAT
AGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGA
AAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGC
CTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCAC
AGCTCCCCTGAAGCCCAGTCTAAGCAGACAGGCAGAGTATTGGAGCCACC
AGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGC
ATCCCGCCCTGCAGAGACCAGCGGAGCCACAGGTTCCTGTGAGAACAACA
TCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGG
GCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGC
CCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGT
GGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCC
TGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCA
AGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCT
GAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGC
ACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAG
TAACGGACTACTCCACATCCAGTGAGGAGTCGGGGACGACGGATGAGGAG
GACGACGATGTGGAGCAGGAACGGGCTGACGAGTCCACCTCAGGACCAGA
GGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAAT
CTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATG
ACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGAGTACAGTTGACCA
AAAGCGTGCCAGCCATCATGAGAGCAATGGCTTTGCCGGTCGCATTCACC
TCTTGCCAGATCTCTTACAGCAAAGCCATTCCTCCTCCACTTCCTCCACC
TCCTCCTCCCCATCCTCCAGCCAGCCGACACCCACCATGTCCCCACAGAC
ACCCCAGGACAAGCTCACTGCTAATGAGACTCAGTCCGCTAGTAGCACAC
TCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGA
TTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGG
ATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCC
GGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGT
GACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCT
GTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCC
TGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAAC
CGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGT
GACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGT
TAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGA
TGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAA
ATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAG
TCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCA
TTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGA
AGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTG
TTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATC
CAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGG
AATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACAT
ATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACA
TCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGC
CATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGC
ACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTG
TTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGAC
CTTAGGCAGGACTTCTCTTCTGAGCTGGTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAND
SPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLV
MEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDE
NPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKD
HIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKE
QRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQH
SQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQS
KQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLS
RRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSS
NSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVF
RPLKPADLTALAKELRAVEDVRPPHKVTDYSTSSEESGTTDEEDDDVEQE
RADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGT
LIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSS
QPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPS
SGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRK
YKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQM
DVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDL
EGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHK
PLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPH
AIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRS
NQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRS
GGSSQVYFMTLGRTSLLSW*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32652
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Ter GST for mammalian expression; ; Estimated MW of expressed protein : 173215 Daltons