cDNA Clone Acylglycerol Kinase

• Plasmid: pET28a AGK MBP
• Parent Plasmid: pET28 MBP
• Expressed: AGK MBP
• Genbank: AAH22777.1
• Tag: MBP
• Species: Human

Sequence of Insert

CCCTTGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATC
GGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGACGGTGTTCT
TTAAAACGCTTCGAAATCACTGGAAGAAAACTACAGCTGGGCTCTGCCTG
CTGACCTGGGGAGGCCATTGGCTCTATGGAAAACACTGTGATAACCTCCT
AAGGAGAGCAGCCTGTCAAGAAGCTCAGGTGTTTGGCAATCAACTCATTC
CTCCCAATGCACAAGTGAAGAAGGCCACTGTTTTTCTCAATCCTGCAGCT
TGCAAAGGAAAAGCTAGGACTCTATTTGAAAAAAATGCTGCCCCGATTTT
ACATTTATCTGGCATGGATGTGACTATTGTTAAGACAGATTATGAGGGAC
AAGCCAAGAAACTCCTGGAACTGATGGAAAACACGGATGTGATCATTGTT
GCAGGAGGAGATGGGACACTGCAGGAGGTTGTTACTGGTGTTCTTCGACG
AACAGATGAGGCTACCTTCAGTAAGATTCCCATTGGATTTATCCCACTGG
GAGAGACCAGTAGTTTGAGTCATACCCTCTTTGCCGAAAGTGGAAACAAA
GTCCAACATATTACTGATGCCACACTTGCCATTGTGAAAGGAGAGACAGT
TCCACTTGATGTCTTGCAGATCAAGGGTGAAAAGGAACAGCCTGTATTTG
CAATGACCGGCCTTCGATGGGGATCTTTCAGAGATGCTGGCGTCAAAGTT
AGCAAGTACTGGTATCTTGGGCCTCTAAAAATCAAAGCAGCCCACTTTTT
CAGCACTCTTAAGGAGTGGCCTCAGACTCATCAAGCCTCTATCTCATACA
CGGGACCTACAGAGAGACCTCCCAATGAACCAGAGGAGACCCCTGTACAA
AGGCCTTCTTTGTACAGGAGAATATTACGAAGGCTTGCGTCCTACTGGGC
ACAACCACAGGATGCCCTTTCCCAAGAGGTGAGCCCGGAGGTCTGGAAAG
ATGTGCAGCTGTCCACCATTGAACTGTCCATCACAACACGGAATAATCAG
CTTGACCCGACAAGCAAAGAAGATTTTCTGAATATCTGCATTGAACCTGA
CACCATCAGCAAAGGAGACTTTATAACTATAGGAAGTCGAAAGGTGAGAA
ACCCCAAGCTGCACGTGGAGGGCACGGAGTGTCTCCAAGCCAGCCAGTGC
ACTTTGCTTATCCCGGAGGGAGCAGGGGGCTCTTTTAGCATTGACAGTGA
GGAGTATGAAGCGATGCCTGTGGAGGTGAAACTGCTCCCCAGGAAGCTGC
AGTTCTTCTGTGATCCTAGGAAGAGAGAACAGATGCTCACAAGCCCCACC
CAGGCGGCCGC

Amino Acid Sequence

MTVFFKTLRNHWKKTTAGLCLLTWGGHWLYGKHCDNLLRRAACQEAQVFG
NQLIPPNAQVKKATVFLNPAACKGKARTLFEKNAAPILHLSGMDVTIVKT
DYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIG
FIPLGETSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKE
QPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQTHQA
SISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSP
EVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEPDTISKGDFITIGS
RKVRNPKLHVEGTECLQASQCTLLIPEGAGGSFSIDSEEYEAMPVEVKLL
PRKLQFFCDPRKREQMLTSPTQAAALEVLFQGPMKIKTGARILALSALTT
MMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPD
KLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYP
FTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKA
KGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAG
LTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVN
YGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLE
AVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWY
AVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU32618
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression; ; Estimated MW of expressed protein : 92787 Daltons