cDNA Clone CERK

• Plasmid: pET28 CERK MBP
• Parent Plasmid: pET28 MBP
• Expressed: CERK MBP
• Genbank: AAH67255
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGTTCAGCGAGGTGCTGCACGGTCTGATTGGGAGGACGCAGAG
GAGCGCCGGGGTCGACCAGAACCACCCCCGGGCTGTGCTGGTCCCCAGTA
GCCTCCGGATTGGAATCATTCCCGCAGGGTCAACGGACTGCGTGTGTTAC
TCCACCGTGGGCACCAGCGACGCAGAAACCTCGGCGCTGCATATCGTTGT
TGGGGACTCGCTGGCCATGGATGTGTCCTCAGTCCACCACAACAGCACAC
TCCTTCGCTACTCCGTGTCCCTGCTGGGCTACGGCTTCTACGGGGACATC
ATCAAGGACAGTGAGAAGAAACGGTGGTTGGGTCTTGCCAGATACGACTT
TTCAGGTTTAAAGACCTTCCTCTCCCACCACTGCTATGAAGGGACAGTGT
CCTTCCTCCCTGCACAACACACGGTGGGATCTCCAAGGGATAGGAAGCCC
TGCCGGGCAGGATGCTTTGTTTGCAGGCAAAGCAAGCAGCAGCTGGAGGA
GGAGCAGAAGAAAGCACTGTATGGTTTGGAAGCTGCGGAGGACGTGGAGG
AGTGGCAAGTCGTCTGTGGGAAGTTTCTGGCCATCAATGCCACAAACATG
TCCTGTGCTTGTCGCCGGAGCCCCAGGGGCCTCTCCCCGGCTGCCCACTT
GGGAGACGGGTCTTCTGACCTCATCCTCATCCGGAAATGCTCCAGGTTCA
ATTTTCTGAGATTTCTCATCAGGCACACCAACCAGCAGGACCAGTTTGAC
TTCACTTTTGTTGAAGTTTATCGCGTCAAGAAATTCCAGTTTACGTCGAA
GCACATGGAGGATGAGGACAGCGACCTCAAGGAGGGGGGGAAGAAGCGCT
TTGGGCACATTTGCAGCAGCCACCCCTCCTGCTGCTGCACCGTCTCCAAC
AGCTCCTGGAACTGCGACGGGGAGGTCCTGCACAGCCCTGCCATCGAGGT
CAGAGTCCACTGCCAGCTGGTTCGACTCTTTGCACGAGGAATTGAAGAGA
ATCCGAAGCCAGACTCACACAGCGCGGCCGC

Amino Acid Sequence

MFSEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPAGSTDCVCYST
VGTSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIK
DSEKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCR
AGCFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSC
ACRRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFT
FVEVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSS
WNCDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHSLEVLFQGPMKI
KTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKF
EKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLA
EITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPK
TWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYD
IKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTIN
GPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAK
EFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKG
EIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNN
NNNLG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU32617
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C ter MBP for bacterial expression; ; Estimated MW of expressed protein : 83218 Daltons