cDNA Clone NEDD4.2b1

• Plasmid: pcDNA5-FRT/TO-HA-2 NEDD4.2B1 C821A
• Parent Plasmid: pcDNA5 FRT/TO HA2
• Expressed: HA nedd4.2b1 C867A
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAG
AAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCCATA
TGCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGAC
ATGGAGCATGGATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCA
CCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAG
TGAACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACT
CAGTGGCACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAA
CATCAGACAGATCAACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCA
GGCACATCAGCGAAGACTTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTC
CCCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAATATCGCTGGAGACTC
TCTCGGTCTGGCTCTGCCCCCACCGCCGGCCTCCCCAGGATCTCGGACCA
GCCCTCAGGAGCTGTCAGAGGAACTATGCAGAAGGCTTCAGATCACTCCA
GACTCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTC
AAGGTTGAGGTCATGCAGTGTCACCGACGCAGTTGCAGAACAGGGCCATC
TACCACCGCCCAGTGCCCCAGCTGGGAGAGCGCGTTCATCAACTGTCACG
GGTGGTGAGGAACCAACGCCATCAGTGGCCTATGTACATACCACGCCGGG
TCTGCCTTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACATACT
ATGTCAATCATAACAATCGAACCACAACTTGGACTCGACCTATCATGCAG
CTTGCAGAAGATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCT
AATCGAGCCTCAGATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAA
CTTTATCTGCCCCGCTGGAGGGTGCCAAGGACTCACCCGTACGTCGGGCT
GTGAAAGACACCCTTTCCAACCCACAGTCCCCACAGCCATCACCTTACAA
CTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCTTGCCGCCCGGCT
GGGAAATGAGGATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAAC
ACAAAGACTACAACCTGGGAAGATCCACGTTTGAAGTTTCCAGTACATAT
GCGGTCAAAGACATCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTG
GCTGGGAAGAAAGAATTCACTTGGATGGCCGAACGTTTTATATTGATCAT
AATAGCAAAATTACTCAGTGGGAAGACCCAAGACTGCAGAACCCAGCTAT
TACTGGTCCGGCTGTCCCTTACTCCAGAGAATTTAAGCAGAAATATGACT
ACTTCAGGAAGAAATTAAAGAAACCTGCTGATATCCCTAATAGGTTTGAA
ATGAAACTTCACAGAGATAACATATTTGAAGAGTCCTATCGGAGAATTAT
GTCCGTGAAAAGACCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTG
AATCAGAGAAAGGTCTTGACTATGGGGGTGTGGCCAGAGAATGGTTCTTC
TTACTGTCCAAAGAGATGTTCAACCCCTACTACGGCCTCTTTGAGTACTC
TGCCACGGACAACTACACCCTTCAGATCAACCCTAATTCAGGCCTCTGTA
ATGAGGATCATTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTG
GCCGTATTTCATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTA
CAAGATGATGTTGGGAAAGCAGATAACCCTGAATGACATGGAATCTGTGG
ATAGTGAATATTACAACTCTTTGAAATGGATCCTGGAGAATGACCCTACT
GAGCTGGACCTCATGTTCTGCATAGACGAAGAAAACTTTGGACAGACATA
TCAAGTGGATTTGAAGCCCAATGGGTCAGAAATAATGGTCACAAATGAAA
ACAAAAGGGAATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGG
GTCCAGAAGCAGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCC
TATTGATTTGATTAAAATTTTTGACGAAAATGAGCTGGAGTTGCTCATGT
GCGGCCTCGGTGATGTGGATGTGAATGACTGGAGACAGCATTCTATTTAC
AAGAACGGCTACTGCCCAAACCACCCCGTCATTCAGTGGTTCTGGAAGGC
TGTGCTACTCATGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTTGTCA
CAGGGACATCGCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCC
AATGGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACT
GCCCAGAGCTCACACAGCCTTTAATCGCCTTGACTTACCTCCATATGAAA
CCTTTGAAGATTTACGAGAGAAACTTCTCATGGCCGTGGAAAATGCTCAA
GGATTTGAAGGGGTGGATTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAERPYTFKDFLLRPRSHKSRVKGFLRLK
MAHMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGW
EEKVNNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRF
RSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPG
SRTSPQELSEELCRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAE
QGHLPPPSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKG
RTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSS
PTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFL
PPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGP
LPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQ
KYDYFRKKLKKPADIPNRFEMKLHRDNIFEESYRRIMSVKRPDVLKARLW
IEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNS
GLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDM
ESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMV
TNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELE
LLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLL
QFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTAFNRLDLP
PYETFEDLREKLLMAVENAQGFEGVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32563
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal HA tag. ; ; Estimated MW of expressed protein : 100370 Daltons