cDNA Clone CDC53 (yeast)

• Plasmid: pFB Dual 6P-1 CDC53 yeast
• Parent Plasmid: pSC-B
• Expressed: GST CDC53
• Genbank: 851424
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGTCTGAGACTCTGCCTAGATCTGATGATTTAGAGGCCACTTGGAACTTTATAGAGCCAGGAATTAACCAAATACTGGGTAATGAGAAAAACCAAGCATCCACGTCTAAGCGAGTTTACAAAATTCTTTCTCCAACCATGTATATGGAAGTCTATACCGCAATTTATAACTACTGTGTGAACAAATCACGCTCGTCTGGACATTTTAGTACTGACAGTAGAACTGGCCAATCAACAATTTTGGTTGGCAGTGAGATTTACGAAAAGTTAAAGAATTATTTGAAGAATTACATTTTAAATTTTAAGCAGTCTAATTCAGAGACCTTTTTGCAGTTTTACGTCAAGCGCTGGAAAAGGTTTACAATAGGTGCCATTTTTTTAAACCATGCATTCGATTATATGAATAGATATTGGGTTCAAAAGGAAAGAAGTGACGGCAAAAGGCATATTTTTGATGTAAACACCTTGTGCTTGATGACATGGAAAGAAGTCATGTTTGATCCGAGTAAAGATGTTTTAATAAACGAATTATTAGACCAGGTAACCTTGGGAAGGGAGGGGCAAATAATTCAAAGAAGTAATATTAGCACTGCCATAAAGTCTTTAGTTGCACTAGGTATCGATCCACAGGATTTGAAAAAGTTGAACCTAAATGTCTACATCCAAGTTTTCGAAAAGCCATTTTTAAAGAAGACTCAGGAGTACTACACGCAATATACAAACGATTATTTAGAGAAACACTCGGTAACT
GAGTATATTTTTGAAGCACATGAAATCATCAAACGTGAGGAAAAAGCAATGACAATATATTGGGATGATCATACAAAAAAACCACTATCCATGGCATTAAACAAGGTCTTGATCACAGACCATATTGAAAAGTTGGAAAATGAGTTTGTTGTCCTTCTGGATGCCAGAGATATTGAAAAAATTACTTCTTTGTACGCACTAATACGCAGAGACTTTACATTAATCCCAAGAATGGCTTCAGTGTTTGAAAATTATGTTAAAAAGACAGGTGAGAATGAAATTTCGAGTCTACTGGCAATGCATAAACACAATATTATGAAAAACGAAAACGCAAACCCTAAAAAACTAGCACTAATGACAGCTCACTCCCTTTCTCCTAAAGACTATATCAAGAAATTACTGGAAGTACACGATATATTCTCTAAGATTTTTAATGAAAGTTTCCCCGACGACATACCTTTAGCTAAAGCTCTAGATAATGCGTGTGGTGCTTTCATCAACATCAACGAGTTCGCATTACCTGCTGGATCTCCAAAAAGTGCCACCTCGAAGACTTCCGAGATGCTAGCTAAGTACAGTGATATACTATTAAAGAAGGCCACCAAACCTGAAGTGGCAAGTGACATGTCAGATGAAGATATTATAACAATATTCAAATATTTGACCGACAAAGATGCGTTCGAAACTCATTATAGAAGACTTTTCGCCAAGCGTTTAATTCATGGCACTTCAACATCAGCAGAAGACGAAGAAAATATTATTCAAAGGCTGCAAGCGGCAAATAGTATGGAATATACAGGCAAGATAACTAAAATGTTTCAAGATATTAGACTTTCCAAGATCTTGGAAGACGATTTTGCTGTCGCCCTGAAGAATGAACCAGATTACTCTAAAGCAAAATATCCAGATCTACAACCATTTGTATTGGCAGAAAATATGTGGCCATTTTCATACCAAGAAGTTGAATTTAAGCTACCCAAGGAATTGGTACCATCTCACGAAAAATTGAAGGAGTCATACAGCCAAAAGCATAATGGTAGAATATTGAAGTGGCTATGGCCATTATGTCGTGGCGAATTGAAGGCAGATATTGGAAAACCCGGCAGGATGCCGTTCAACTTTACGGTAACACTGTTTCAAATGGCAATTTTGCTACTATATAACGATGCTGATGTATTAACCTTAGAAAATATTCAAGAGGGCACGAGCTTAACCATTCAGCACATCGCAGCGGCAATGGTACCATTCATCAAATTCAAACTGATTCAACAGGTCCCACCAGGGCTAGATGCTCTCGTGAAACCTGAAACACAATTCAAGCTATCACGTCCTTATAAGGCGCTTAAGACCAATATTAATTTCGCTAGCGGAGTCAAAAACGACATTCTGCAATCGCTATCCGGTGGAGGCCACGATAATCACGGTAATAAGTTAGGAAACAAACGCTTGACGGAAGACGAAAGAATAGAAAAAGAATTGAATACAGAAAGGCAGATTTTCTTGGAAGCTTGTATTGTGAGAATAATGAAAGCGAAAAGAAATCTGCCACATACAACATTGGTCAACGAATGCATTGCGCAATCACACCAAAGGTTCAATGCAAAGGTTTCTATGGTCAAGAGAGCCATCGATAGCTTAATACAAAAGGGATACCTACAGAGGGGAGACGATGGTGAATCGTATGCTTACCTGCTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSETLPRSDDLEATWNFIEPGINQILGNEKNQASTSKRVYKILSPTMYMEVYTAIYNYCVNKSRSSGHFSTDSRTGQSTILVGSEIYEKLKNYLKNYILNFKQSNSETFLQFYVKRWKRFTIGAIFLNHAFDYMNRYWVQKERSDGKRHIFDVNTLCLMTWKEVMFDPSKDVLINELLDQVTLGREGQIIQRSNISTAIKSLVALGIDPQDLKKLNLNVYIQVFEKPFLKKTQEYYTQYTNDYLEKHSVTEYIFEAHEIIKREEKAMTIYWDDHTKKPLSMALNKVLITDHIEKLENEFVVLLDARDIEKITSLYALIRRDFTLIPRMASVFENYVKKTGENEISSLLAMHKHNIMKNENANPKKLALMTAHSLSPKDYIKKLLEVHDIFSKIFNESFPDDIPLAKALDNACGAFININEFALPAGSPKSATSKTSEMLAKYSDILLKKATKPEVASDMSDEDIITIFKYLTDKDAFETHYRRLFAKRLIHGTSTSAEDEENIIQRLQAANSMEYTGKITKMFQDIRLSKILEDDFAVALKNEPDYSKAKYPDLQPFVLAENMWPFSYQEVEFKLPKELVPSHEKLKESYSQKHNGRILKWLWPLCRGELKADIGKPGRMPFNFTVTLFQMAILLLYNDADVLTLENIQEGTSLTIQHIAAAMVPFIKFKLIQQVPPGLDALVKPETQFKLSRPYKALKTNINFASGVKNDILQSLSGGGHDNHGNKLGNKRLTEDERIEKELNTERQIFLEACIVRIMKAKRNLPHTTLVNECIAQSHQRFNAKVSMVKRAIDSLIQKGYLQRGDDGESYAYLLKRP

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32477
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 121206.43 Daltons