cDNA Clone NEDD8

• Synonyms: NEDD-8
• Plasmid: pET156P NEDD8 M1-G76
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C NEDD8 M1-G76
• Genbank: Q15843
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCTAATTAAAGTGAAGACGCTGACCGGAAAGGAGATTGAGAT
TGACATTGAACCTACAGACAAGGTGGAGCGAATCAAGGAGCGTGTGGAGG
AGAAAGAGGGAATCCCCCCACAACAGCAGAGGCTCATCTACAGTGGCAAG
CAGATGAATGATGAGAAGACAGCAGCTGATTACAAGATTTTAGGTGGTTC
AGTCCTTCACCTGGTGTTGGCTCTGAGAGGAGGATAAGGTCTTAGGCAGT
GAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMLIKVKTLTGKEIEIDIEPTDKVERIK
ERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET156P NEDD8 M1-G76102.03 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU32475
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression; ; Estimated MW of expressed protein : 11004 Daltons
• Low Temp: No