cDNA Clone MAP3K13

• Plasmid: pET156P MAP3K13
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His MAP3K13
• Genbank: NM_004721.3
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCAACTTTCAGGAGCACCTGAGCTGCTCCTCTTCTCC
ACACTTACCCTTCAGTGAAAGCAAAACCTTCAATGGACTACAAGATGAGC
TCACAGCTATGGGGAACCACCCTTCTCCCAAGCTGCTCGAGGACCAGCAG
GAAAAGGGGATGGTACGAACAGAGCTAATCGAGAGCGTGCACAGCCCCGT
CACCACAACAGTGTTGACGAGCGTAAGTGAGGACTCCAGGGACCAGTTTG
AGAACAGCGTTCTTCAGCTAAGGGAACACGATGAATCAGAGACGGCGGTG
TCTCAGGGGAACAGCAACACGGTGGACGGAGAGAGCACAAGCGGAACTGA
AGACATAAAGATTCAGTTCAGCAGGTCAGGCAGTGGCAGTGGTGGGTTTC
TTGAAGGACTATTTGGATGCTTAAGGCCTGTATGGAATATCATTGGGAAG
GCATATTCCACTGATTACAAATTGCAGCAGCAAGATACTTGGGAAGTGCC
ATTTGAGGAGATCTCAGAGCTGCAGTGGCTGGGTAGTGGAGCCCAAGGAG
CGGTCTTCTTGGGCAAGTTCCGGGCGGAAGAGGTGGCCATCAAGAAAGTG
AGAGAACAGAATGAGACGGATATCAAGCATTTGAGGAAGTTGAAGCACCC
TAACATCATCGCATTCAAGGGTGTTTGTACTCAGGCCCCATGTTATTGTA
TTATCATGGAATACTGTGCCCATGGACAACTCTACGAGGTCTTACGAGCT
GGCAGGAAGATCACACCTCGATTGCTAGTAGACTGGTCCACAGGAATTGC
AAGTGGAATGAATTATTTGCACCTCCATAAAATTATTCATCGTGATCTCA
AATCACCTAATGTTTTAGTGACCCACACAGATGCGGTAAAAATTTCAGAT
TTTGGTACATCTAAGGAACTCAGTGACAAAAGTACCAAGATGTCATTTGC
TGGCACGGTCGCATGGATGGCGCCAGAGGTGATACGGAATGAACCTGTCT
CTGAAAAAGTTGATATATGGTCTTTTGGAGTGGTGCTTTGGGAGCTGCTG
ACAGGAGAGATCCCTTACAAAGACGTAGATTCTTCAGCCATTATCTGGGG
TGTTGGAAGCAACAGCCTCCACCTTCCAGTTCCTTCCACTTGCCCTGATG
GATTCAAAATCCTTATGAAACAGACGTGGCAGAGTAAACCTCGAAACCGA
CCTTCTTTTCGGCAGACACTCATGCATTTAGACATTGCCTCTGCAGATGT
ACTTGCCACCCCACAAGAAACTTACTTCAAGTCTCAGGCTGAATGGAGAG
AAGAAGTGAAAAAACATTTTGAGAAGATCAAAAGTGAAGGAACTTGTATA
CACCGGTTAGATGAAGAACTGATTCGAAGGCGCAGAGAAGAGCTCAGGCA
TGCGCTGGATATTCGTGAACACTATGAGCGGAAGCTTGAGCGGGCGAATA
ATTTATACATGGAATTGAGTGCCATCATGCTGCAGCTAGAAATGCGGGAG
AAGGAGCTCATTAAGCGTGAGCAAGCAGTGGAAAAGAAGTATCCTGGGAC
CTACAAACGACACCCTGTTCGTCCTATCATCCATCCCAATGCCATGGAGA
AACTCATGAAAAGGAAAGGAGTGCCTCACAAATCTGGGATGCAGACCAAA
CGGCCAGACTTGTTGAGATCAGAAGGGATCCCCACCACAGAAGTGGCTCC
CACTGCATCCCCTTTGTCCGGAAGTCCCAAAATGTCCACTTCTAGCAGCA
AGAGCCGATATCGAAGCAAACCACGCCACCGCCGAGGGAATAGCAGAGGC
AGCCATAGTGACTTTGCCGCAATCTTGAAAAACCAGCCAGCCCAGGAAAA
TTCACCCCATCCCACTTACCTGCACCAAGCTCAATCCCAATACCCTTCTC
TTCATCACCATAATTCTCTGCAGCAGCAATACCAGCAGCCCCCTCCTGCC
ATGTCCCAGAGTCACCATCCCAGACTCAATATGCACGGACAGGACATAGC
AACCTGCGCCAACAACCTGAGGTATTTCGGCCCAGCAGCAGCCCTGCGGA
GCCCACTCAGCAACCATGCTCAGAGACAGCTGCCCGGCTCGAGCCCTGAC
CTCATCTCCACAGCCATGGCTGCAGACTGCTGGAGAAGTTCTGAGCCTGA
CAAGGGCCAAGCTGGTCCCTGGGGCTGTTGCCAGGCTGACGCTTATGACC
CCTGCCTTCAGTGCAGGCCAGAACAGTATGGGTCCTTAGACATACCCTCT
GCTGAGCCAGTGGGGAGGAGCCCTGACCTTTCCAAGTCACCAGCACATAA
TCCTCTCTTGGAAAACGCCCAGAGTTCTGAGAAAACGGAAGAAAATGAAT
TCAGCGGCTGTAGGTCTGAGTCATCCCTCGGCACCTCTCATCTCGGCACC
CCTCCAGCGCTACCTCGAAAAACAAGGCCTCTGCAGAAGAGTGGAGATGA
CTCCTCAGAAGAGGAAGAAGGGGAAGTAGATAGTGAAGTTGAATTTCCAC
GAAGACAGAGGCCCCATCGCTGTATCAGCAGCTGCCAGTCATATTCAACC
TTTAGCTCTGAGAATTTCTCTGTGTCTGATGGAGAAGAGGGAAATACCAG
TGACCACTCAAACAGTCCTGATGAGTTAGCTGATAAACTTGAAGACCGCT
TGGCAGAGAAGCTAGACGACCTGCTGTCCCAGACGCCAGAGATTCCCATT
GACATATCCTCACACTCGGATGGGCTCTCTGACAAGGAGTGTGCCGTGCG
CCGTGTGAAGACTCAGATGTCTCTGGGCAAGCTGTGTGTGGAGGAACGTG
GCTATGAGAACCCCATGCAGTTTGAAGAATCGGACTGTGACTCTTCAGAT
GGGGAGTGTTCTGATGCCACAGTTAGGACCAATAAACACTACAGCTCTGC
TACCTGGTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMANFQEHLSCSSSPH
LPFSESKTFNGLQDELTAMGNHPSPKLLEDQQEKGMVRTELIESVHSPVT
TTVLTSVSEDSRDQFENSVLQLREHDESETAVSQGNSNTVDGESTSGTED
IKIQFSRSGSGSGGFLEGLFGCLRPVWNIIGKAYSTDYKLQQQDTWEVPF
EEISELQWLGSGAQGAVFLGKFRAEEVAIKKVREQNETDIKHLRKLKHPN
IIAFKGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIAS
GMNYLHLHKIIHRDLKSPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAG
TVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGV
GSNSLHLPVPSTCPDGFKILMKQTWQSKPRNRPSFRQTLMHLDIASADVL
ATPQETYFKSQAEWREEVKKHFEKIKSEGTCIHRLDEELIRRRREELRHA
LDIREHYERKLERANNLYMELSAIMLQLEMREKELIKREQAVEKKYPGTY
KRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPT
ASPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENS
PHPTYLHQAQSQYPSLHHHNSLQQQYQQPPPAMSQSHHPRLNMHGQDIAT
CANNLRYFGPAAALRSPLSNHAQRQLPGSSPDLISTAMAADCWRSSEPDK
GQAGPWGCCQADAYDPCLQCRPEQYGSLDIPSAEPVGRSPDLSKSPAHNP
LLENAQSSEKTEENEFSGCRSESSLGTSHLGTPPALPRKTRPLQKSGDDS
SEEEEGEVDSEVEFPRRQRPHRCISSCQSYSTFSSENFSVSDGEEGNTSD
HSNSPDELADKLEDRLAEKLDDLLSQTPEIPIDISSHSDGLSDKECAVRR
VKTQMSLGKLCVEERGYENPMQFEESDCDSSDGECSDATVRTNKHYSSAT
W

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32355
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression expression; ; Estimated MW of expressed : 111907 Daltons