cDNA Clone GFAT1

• Plasmid: pET24a GFAT1 1-300 6his 301-681
• Parent Plasmid: pET24a original
• Expressed: GFAT1 1-300 6His 301-681
• Genbank: NP_002047.2
• Tag: 6His
• Species: Human

Sequence of Insert

ACTAGTAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGTGTGGT
ATATTTGCTTACTTAAACTACCATGTTCCTCGAACGAGACGAGAAATCCT
GGAGACCCTAATCAAAGGCCTTCAGAGACTGGAGTACAGAGGATATGATT
CTGCTGGTGTGGGATTTGATGGAGGCAATGATAAAGATTGGGAAGCCAAT
GCCTGCAAAATCCAGCTTATTAAGAAGAAAGGAAAAGTTAAGGCACTGGA
TGAAGAAGTTCACAAGCAACAAGATATGGATTTGGATATAGAATTTGATG
TACACCTTGGAATAGCTCATACCCGTTGGGCAACACATGGAGAACCCAGT
CCTGTCAATAGCCACCCCCAGCGCTCTGATAAAAATAATGAATTTATCGT
TATTCACAATGGAATCATCACCAACTACAAAGACTTGAAAAAGTTTTTGG
AAAGCAAAGGCTATGACTTCGAATCTGAAACAGACACAGAGACAATTGCC
AAGCTCGTTAAGTATATGTATGACAATCGGGAAAGTCAAGATACCAGCTT
TACTACCTTGGTGGAGAGAGTTATCCAACAATTGGAAGGTGCTTTTGCAC
TTGTGTTTAAAAGTGTTCATTTTCCCGGGCAAGCAGTTGGCACAAGGCGA
GGTAGCCCTCTGTTGATTGGTGTACGGAGTGAACATAAACTTTCTACTGA
TCACATTCCTATACTCTACAGAACAGGCAAAGACAAGAAAGGAAGCTGCA
ATCTCTCTCGTGTGGACAGCACAACCTGCCTTTTCCCGGTGGAAGAAAAA
GCAGTGGAGTATTACTTTGCTTCTGATGCAAGTGCTGTCATAGAACACAC
CAATCGCGTCATCTTTCTGGAAGATGATGATGTTGCAGCAGTAGTGGATG
GACGTCTTTCTATCCATCGAATTAAACGAACTGCAGGAGATCATCATCAC
CATCACCATCACCCCGGACGAGCTGTGCAAACACTCCAGATGGAACTCCA
GCAGATCATGAAGGGCAACTTCAGTTCATTTATGCAGAAGGAAATATTTG
AGCAGCCAGAGTCTGTCGTGAACACAATGAGAGGAAGAGTCAACTTTGAT
GACTATACTGTGAATTTGGGTGGTTTGAAGGATCACATAAAGGAGATCCA
GAGATGCCGGCGTTTGATTCTTATTGCTTGTGGAACAAGTTACCATGCTG
GTGTAGCAACACGTCAAGTTCTTGAGGAGCTGACTGAGTTGCCTGTGATG
GTGGAACTAGCAAGTGACTTCCTGGACAGAAACACACCAGTCTTTCGAGA
TGATGTTTGCTTTTTCCTTAGTCAATCAGGTGAGACAGCAGATACTTTGA
TGGGTCTTCGTTACTGTAAGGAGAGAGGAGCTTTAACTGTGGGGATCACA
AACACAGTTGGCAGTTCCATATCACGGGAGACAGATTGTGGAGTTCATAT
TAATGCTGGTCCTGAGATTGGTGTGGCCAGTACAAAGGCTTATACCAGCC
AGTTTGTATCCCTTGTGATGTTTGCCCTTATGATGTGTGATGATCGGATC
TCCATGCAAGAAAGACGCAAAGAGATCATGCTTGGATTGAAACGGCTGCC
TGATTTGATTAAGGAAGTACTGAGCATGGATGACGAAATTCAGAAACTAG
CAACAGAACTTTATCATCAGAAGTCAGTTCTGATAATGGGACGAGGCTAT
CATTATGCTACTTGTCTTGAAGGGGCACTGAAAATCAAAGAAATTACTTA
TATGCACTCTGAAGGCATCCTTGCTGGTGAATTGAAACATGGCCCTCTGG
CTTTGGTGGATAAATTGATGCCTGTGATCATGATCATCATGAGAGATCAC
ACTTATGCCAAGTGTCAGAATGCTCTTCAGCAAGTGGTTGCTCGGCAGGG
GCGGCCTGTGGTAATTTGTGATAAGGAGGATACTGAGACCATTAAGAACA
CAAAAAGAACGATCAAGGTGCCCCACTCTGTGGACTGCTTGCAGGGCATT
CTCAGCGTGATCCCTTTACAGTTGCTGGCTTTCCACCTTGCTGTGCTGAG
AGGCTATGATGTTGATTTCCCACGGAATCTTGCCAAATCTGTGACTGTAG
AGTGACATTTGCGGCCGC

Amino Acid Sequence

MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDW
EANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHG
EPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTE
TIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVG
TRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPV
EEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGD
HHHHHHHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRV
NFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTEL
PVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTV
GITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCD
DRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMG
RGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIM
RDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCL
QGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU32294
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Internal 6HIS for bacterial expression; ; Estimated MW of expressed protein : 77579 Daltons
• Low Temp: No