cDNA Clone DMPK

• Synonyms: myotonin-protein kinase isoform 2, DM, DM1, DM1PK, DMK, MDPK, MT-PK
• Plasmid: pFBDual 6P-2 DMPK
• Parent Plasmid: pFastbacDual 6P2
• Expressed: GST DMPK
• Genbank: NP_004400
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTCAGCCGAGGTGCGGCTGAGGCGGCTCCAGCAGCTGGT
GTTGGACCCGGGCTTCCTGGGGCTGGAGCCCCTGCTCGACCTTCTCCTGG
GCGTCCACCAGGAGCTGGGCGCCTCCGAACTGGCCCAGGACAAGTACGTG
GCCGACTTCTTGCAGTGGGCGGAGCCCATCGTGGTGAGGCTTAAGGAGGT
CCGACTGCAGAGGGACGACTTCGAGATTCTGAAGGTGATCGGACGCGGGG
CGTTCAGCGAGGTAGCGGTAGTGAAGATGAAGCAGACGGGCCAGGTGTAT
GCCATGAAGATCATGAACAAGTGGGACATGCTGAAGAGGGGCGAGGTGTC
GTGCTTCCGTGAGGAGAGGGACGTGTTGGTGAATGGGGACCGGCGGTGGA
TCACGCAGCTGCACTTCGCCTTCCAGGATGAGAACTACCTGTACCTGGTC
ATGGAGTATTACGTGGGCGGGGACCTGCTGACACTGCTGAGCAAGTTTGG
GGAGCGGATTCCGGCCGAGATGGCGCGCTTCTACCTGGCGGAGATTGTCA
TGGCCATAGACTCGGTGCACCGGCTTGGCTACGTGCACAGGGACATCAAA
CCCGACAACATCCTGCTGGACCGCTGTGGCCACATCCGCCTGGCCGACTT
CGGCTCTTGCCTCAAGCTGCGGGCAGATGGAACGGTGCGGTCGCTGGTGG
CTGTGGGCACCCCAGACTACCTGTCCCCCGAGATCCTGCAGGCTGTGGGC
GGTGGGCCTGGGACAGGCAGCTACGGGCCCGAGTGTGACTGGTGGGCGCT
GGGTGTATTCGCCTATGAAATGTTCTATGGGCAGACGCCCTTCTACGCGG
ATTCCACGGCGGAGACCTATGGCAAGATCGTCCACTACAAGGAGCACCTC
TCTCTGCCGCTGGTGGACGAAGGGGTCCCTGAGGAGGCTCGAGACTTCAT
TCAGCGGTTGCTGTGTCCCCCGGAGACACGGCTGGGCCGGGGTGGAGCAG
GCGACTTCCGGACACATCCCTTCTTCTTTGGCCTCGACTGGGATGGTCTC
CGGGACAGCGTGCCCCCCTTTACACCGGATTTCGAAGGTGCCACCGACAC
ATGCAACTTCGACTTGGTGGAGGACGGGCTCACTGCCATGGTGAGCGGGG
GCGGGGAGACACTGTCGGACATTCGGGAAGGTGCGCCGCTAGGGGTCCAC
CTGCCTTTTGTGGGCTACTCCTACTCCTGCATGGCCCTCAGGGACAGTGA
GGTCCCAGGCCCCACACCCATGGAACTGGAGGCCGAGCAGCTGCTTGAGC
CACACGTGCAAGCGCCCAGCCTGGAGCCCTCGGTGTCCCCACAGGATGAA
ACAGCTGAAGTGGCAGTTCCAGCGGCTGTCCCTGCGGCAGAGGCTGAGGC
CGAGGTGACGCTGCGGGAGCTCCAGGAAGCCCTGGAGGAGGAGGTGCTCA
CCCGGCAGAGCCTGAGCCGGGAGATGGAGGCCATCCGCACGGACAACCAG
AACTTCGCCAGTCAACTACGCGAGGCAGAGGCTCGGAACCGGGACCTAGA
GGCACACGTCCGGCAGTTGCAGGAGCGGATGGAGTTGCTGCAGGCAGAGG
GAGCCACAGCTGTCACGGGGGTCCCCAGTCCCCGGGCCACGGATCCACCT
TCCCATCTAGATGGCCCCCCGGCCGTGGCTGTGGGCCAGTGCCCGCTGGT
GGGGCCAGGCCCCATGCACCGCCGCCACCTGCTGCTCCCTGCCAGGGTCC
CTAGGCCTGGCCTATCGGAGGCGCTTTCCCTGCTCCTGTTCGCCGTTGTT
CTGTCTCGTGCCGCCGCCCTGGGCTGCATTGGGTTGGTGGCCCACGCCGG
CCAACTCACCGCAGTCTGGCGCCGCCCAGGAGCCGCCCGCGCTCCCTGAG
CGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMSAEVRLR
RLQQLVLDPGFLGLEPLLDLLLGVHQELGASELAQDKYVADFLQWAEPIV
VRLKEVRLQRDDFEILKVIGRGAFSEVAVVKMKQTGQVYAMKIMNKWDML
KRGEVSCFREERDVLVNGDRRWITQLHFAFQDENYLYLVMEYYVGGDLLT
LLSKFGERIPAEMARFYLAEIVMAIDSVHRLGYVHRDIKPDNILLDRCGH
IRLADFGSCLKLRADGTVRSLVAVGTPDYLSPEILQAVGGGPGTGSYGPE
CDWWALGVFAYEMFYGQTPFYADSTAETYGKIVHYKEHLSLPLVDEGVPE
EARDFIQRLLCPPETRLGRGGAGDFRTHPFFFGLDWDGLRDSVPPFTPDF
EGATDTCNFDLVEDGLTAMVSGGGETLSDIREGAPLGVHLPFVGYSYSCM
ALRDSEVPGPTPMELEAEQLLEPHVQAPSLEPSVSPQDETAEVAVPAAVP
AAEAEAEVTLRELQEALEEEVLTRQSLSREMEAIRTDNQNFASQLREAEA
RNRDLEAHVRQLQERMELLQAEGATAVTGVPSPRATDPPSHLDGPPAVAV
GQCPLVGPGPMHRRHLLLPARVPRPGLSEALSLLLFAVVLSRAAALGCIG
LVAHAGQLTAVWRRPGAARAP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32255
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression; ; Estimated MW of expressed protein : 97184 Daltons