cDNA Clone NEDD4

• Plasmid: pGEX6P-1-Nedd4
• Parent Plasmid: pGEX6P1
• Expressed: GST NEDD4
• Genbank: NM_006154.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGA
GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG
CCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTA
TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA
AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC
CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA
AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC
AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC
ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG
ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC
TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT
GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA
GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG
AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG
AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG
CAGATATCCGAGGAAACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAA
CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAGGCCT
TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA
GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA
GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT
ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT
GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA
TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC
AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC
CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC
ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG
CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC
TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC
ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA
GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA
ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC
AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA
AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC
CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG
AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG
GATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG
GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA
TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC
TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT
GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT
TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT
ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC
AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT
GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT
ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA
ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT
CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG
ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC
AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT
GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC
GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG
TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA
TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT
TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA
GTTGATTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATCAVEVFGLLEDEENSR
IVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLN
PKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPR
LERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELE
PGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQW
KRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNQESSENWEII
REDEATMYSSQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASS
SNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDH
NSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIE
QGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTS
NDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYS
RDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFL
KARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQ
INPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPI
TLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGG
SEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFD
ENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEK
RIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFN
RLDLPPYESFEELWDKLQMAIENTQGFDGVD

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32244
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: NCBI since updated (NM_006154.3). This clone has R260Q and N279S mutations compared to updated NCBI. Also has silent mutation at Thr857 (ACT>ACG); ; Estimated MW of expressed protein : 130983 Daltons