cDNA Clone NEDD8

• Plasmid: pMalP NEDD8 1-76
• Parent Plasmid: pMAL6P
• Expressed: MBP NEDD8 1-76
• Genbank: Q15843
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGCTAATTAAAGTGAAGACGCTGACCGGAAAGGAGATTGAGAT
TGACATTGAACCTACAGACAAGGTGGAGCGAATCAAGGAGCGTGTGGAGG
AGAAAGAGGGAATCCCCCCACAACAGCAGAGGCTCATCTACAGTGGCAAG
CAGATGAATGATGAGAAGACAGCAGCTGATTACAAGATTTTAGGTGGTTC
AGTCCTTCACCTGGTGTTGGCTCTGAGAGGAGGATAAGGTCTTAGGCAGT
GAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMLIKVKTLTGKEIEIDIEPTDK
VERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLA
LRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32136
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression; ; Estimated MW of expressed protein : 55297 Daltons